Analysis of raw meats and fats of pigs using polymerase chain reaction for Halal authentication

Aida, A.A.; Man, Y. B. Che; Wong, Clemente Michael Vui Ling; Raha, Abdul Rahim; Radu, Son

doi:10.1016/j.meatsci.2004.06.020

Cited by 169 publications

(103 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…While the possibility of cross contamination with pork cannot be excluded in these cases, there appears to be an increasing tendency for food manufacturers to mix readily available pork flesh or derivatives into meat products to accrue greater profits Aida, Che Man, Wong, Raha, & Son, 2005). Animal fats from one species are also reported to be frequently substituted with those from other species (Ballin, 2010).…”

Section: Discussionmentioning

confidence: 99%

A high incidence of species substitution and mislabelling detected in meat products sold in South Africa

Steinman²,

2013

View full text Add to dashboard Cite

Due to their high market value, meat products are often targets for species substitution and adulteration. DNA-based methods are recognized as the most appropriate means to detect such fraudulent practices, however, these have not been extensively employed for the authentication of meat products available in South Africa. The aim of this study was to utilize a variety of molecular techniques to evaluate the extent of meat product mislabelling prevailing on the local market. A total of 139 processed meat products (minced meats, burger patties, deli meats, sausages and dried meats) were collected from retail outlets and butcheries in South Africa. The enzyme-linked immunosorbent assay (ELISA) was employed for the detection of undeclared plant proteins (soya and gluten) in the samples. A commercial DNA-based LCD array was used to screen the samples for the presence of 14 animal species, the results of which were confirmed by species-specific polymerase chain reaction (PCR) and in some cases also DNA sequencing. The results revealed that 95 of 139 (68%) samples contained species which were not declared on the product labelling, with the incidence being highest in sausages, burger patties and deli meats. Soya and gluten were identified as undeclared plant proteins in a large number of samples (>28%), while pork (37%) and chicken (23%) were the most commonly detected animal species. Unconventional species such as donkey, goat and water buffalo were also discovered in a number of products. Overall, this study confirmed that the mislabelling of processed meats is commonplace in South Africa and not only violates food labelling regulations, but also poses economic, religious, ethical and health impacts.

show abstract

Section: Discussionmentioning

confidence: 99%

A high incidence of species substitution and mislabelling detected in meat products sold in South Africa

Steinman²,

2013

View full text Add to dashboard Cite

show abstract

“…Another approach is to use the PCR technique. [3,7,8] The PCR technique can be used to analyze a biological food such as meat. This technique has a good sensitivity in detecting the existence of very small and small samples.…”

Section: Introductionmentioning

confidence: 99%

“…This technique has a good sensitivity in detecting the existence of very small and small samples. [6,8] In addition, the cost required to perform the technique is relatively cheaper than the protein. The process is relatively more practical than protein-based methods.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Species in Meat Products in Bandung City using Multiplex PCR

Yelliantty

Rohima²

2018

PFTJ

View full text Add to dashboard Cite

Cases concerning the substitution of meat raw material also occur in Indonesia and are quite common. Therefore, careful monitoring and control that needs to be done on the meat products. Screening or sampling products on the market should be conducted periodically to ensure the safety of consumers and society in general. Such screening should be done accurately. This study aimed to analyze the composition of meat in processed products in traditional markets in Bandung using PCR method. This study was using four specific primers to detect four different species. Screening is done on samples of meatballs from several markets. The results showed the presence of several samples that contain meat of some species. based it can be concluded that the substitution of raw materials processed meat products also occurs in the traditional market in the city of Bandung, and the PCR method referred to can be used as the basis for the development of detection methods of food security in Indonesia.

show abstract

“…In Islam, food adulteration is when the food was added with prohibited (Haram) substances for Muslim to consume and also alter the food quality [1]. Consumption of porcine derivatives is prohibited according to the Islamic law [2].…”

Section: Introductionmentioning

confidence: 99%

“…For Muslim consumers, the major authenticity concerns in meat and meat products include pork substitution, undeclared blood plasma, and use of prohibited ingredients, pork intestine casings and non-halal methods of slaughter. But in most countries, food manufactures choose to use porcine derivatives because they are cheap and readily available [1]. Porcine derivatives used in the meat processing industry include; pork fat (lard), mechanically recovered meats (MRM), porcine gelatine and porcine blood plasma.…”

Section: Introductionmentioning

confidence: 99%

Comparison between Mt-DNA D-Loop and Cyt B primers for porcine DNA detection in meat products

Hamzah

Mutalib²,

Babji³

2013

AIP Conference Proceedings

View full text Add to dashboard Cite

Analysis of raw meats and fats of pigs using polymerase chain reaction for Halal authentication

Cited by 169 publications

References 25 publications

A high incidence of species substitution and mislabelling detected in meat products sold in South Africa

A high incidence of species substitution and mislabelling detected in meat products sold in South Africa

Analysis of Species in Meat Products in Bandung City using Multiplex PCR

Comparison between Mt-DNA D-Loop and Cyt B primers for porcine DNA detection in meat products

Contact Info

Product

Resources

About