Analysis of Reciprocally Dysregulated miRNAs in Eutopic Endometrium Is a Promising Approach for Low Invasive Diagnostics of Adenomyosis

Abstract

Endometriosis is a chronic disease characterized by the growth of endometrial tissue outside of the uterine cavity. Endometriosis affects up to 10% of women of reproductive age and has great social impact. The diagnostics of endometriosis are based on clinical appearance, ultrasound, and magnetic resonance imaging (MRI); however, a diagnosis is frequently hampered by the absence of objective criteria. Adenomyosis (AM) is a particular type of endometriosis wherein the spread of the ectopic endometrial gland is limited by the uterine myometrium. Alteration of the microRNA expression profile in the eutopic endometrium can be associated with AM, and may be assayed for diagnostic purposes. In the presented study, we aimed to explore the diagnostic potency of this approach. Eutopic endometrium specimens were collected from patients (n = 33) and healthy women (n = 30). The microRNA expression was profiled to select individual microRNAs with AM-associated expression alterations. A new method of two-tailed RT-qPCR microRNA analysis was applied to assay potential markers. The expression ratios of reciprocally dysregulated microRNAs were calculated, and the diagnostic potency of these parameters was evaluated by receiver operation curve (ROC) analysis. Mir-10b, miR-200c and miR-191 were significantly dysregulated in the eutopic endometrium of AM patients. The expression ratio of reciprocally dysregulated microRNAs allowed us to diagnose AM with a range of sensitivity from 65% to 74%, and of specificity from 72% to 86%. The analysis of microRNAs from the eutopic endometrium might present a promising low-invasive method of AM diagnostics.

MIR503HG silencing promotes endometrial stromal cell progression and metastasis and suppresses apoptosis in adenomyosis by activating the Wnt/β‑catenin pathway via targeting miR‑191

“…Eutopic endometrium of patients with AD exhibits reciprocally dysregulated miRNAs such as upregulated miR-191, the analysis of which may be a promising low-invasive method for efficient diagnosis of AD ( 20 ). In addition, miRNAs participate in lncRNA-mediated AD progression by serving as the downstream targets of lncRNAs ( 13 ).…”

“…Meanwhile, at only ~22 nucleotides in length and also classified as ncRNAs, microRNAs (miRNAs or miRs) have been discovered to act as a downstream mechanism of lncRNA-mediated changes in processes of ESCs during AD progression ( 13 ). Among them, miR-191 is notably dysregulated in the endometrium tissues of patients with AD ( 20 ). Therefore, identifying downstream miRNAs of lncRNAs that regulate AD is key.…”

Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia

“…Анализ прогностической значимости реципрокных пар микроРНК. Феномен сочетанных и разнонаправленных (реципрокных) изменений экспрессии 2 молекул ми-кроРНК в процессе развития онкологических заболеваний был описан ранее [17,38,39]. В ряде исследований было показано, что анализ соотношения концентраций 2 молекул микроРНК, для которых характерны разнонаправленные опухольассоциированные изменения экспрессионной активности, может иметь высокий диагностический потенциал.…”

Circ_0061140 Facilitates Adenomyosis Progression by Upregulating LIN28B in vitro

“…Noncoding RNAs, containing microRNAs (miRNAs), long noncoding RNAs, and circular RNAs (circRNAs), are pivotal molecules in regulating the pathology of human diseases [11]. Accruing articles have indicated that miRNAs and long noncoding RNAs exerted pivotal functions in adenomyosis development [12][13][14]. Nevertheless, the roles of circRNAs in adenomyosis progression have been rarely studied.…”