Analysis of relationship between cell cycle stage and apoptosis induction in K562 cells by sedimentation field-flow fractionation

Bertrand, J.; Liagre, Bertrand; Bégaud-Grimaud, G.; Jauberteau, Marie‐Odile; Beneytout, Jean-Louis; Cardot, Philippe; Battu, Serge

doi:10.1016/j.jchromb.2009.02.064

Cited by 17 publications

(23 citation statements)

References 69 publications

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“…G 0 is a phase of resting cells outside the cell cycle. Active cell cycle phases include G 1 (gap 1), S (synthesis of DNA), G 2 (gap 2), and M (mitosis) [11]. We showed, in the present study, that molybdenum is a cytotoxic agent and can cause S-phase cell cycle arrest which similar to the study of fluoride on primary cultured rat hippocampal neurons [12].…”

Section: Discussionsupporting

confidence: 86%

Effect of Dietary High Molybdenum on the Cell Cycle and Apoptosis of Kidney in Broilers

Xiao

Cui

Yang

et al. 2010

Biol Trace Elem Res

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The experiment was conducted with the objective of examining the effects of high molybdenum on the cell cycle and apoptosis of kidney in broilers by the methods of flow cytometry. Three hundred 1-day-old Avian broilers were randomly divided into four groups, and fed on diets as follows: control diet (Mo 13 mg/kg) and high molybdenum diets (Mo 500 mg/kg, high molybdenum group I; Mo 1,000 mg/kg, high molybdenum group II; Mo 1,500 mg/kg, high molybdenum group III) for 6 weeks. The results showed that the relative weight of kidney were higher (P < 0.05 or P < 0.01), and the cellular percentages of G(0)/G(1) phase were lower, and cellular percentages of S phase and the proliferating index were higher in high molybdenum groups II and III than in control group (P < 0.01). The percentage of renal cell apoptosis was increased in high molybdenum groups II and III when compared with that of control group (P < 0.01). Immunohistochemical test showed that there were increased frequencies of positive cells containing Bax protein and decreased frequencies of positive cells containing Bcl-2 protein in high molybdenum groups II and III. It was concluded that dietary high molybdenum (1,000 mg/kg and 1,500 mg/kg) impaired the progression of renal cells from S phase to G(2)M phase obviously and induced renal cell apoptosis.

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Section: Discussionsupporting

confidence: 86%

Effect of Dietary High Molybdenum on the Cell Cycle and Apoptosis of Kidney in Broilers

Xiao

Cui

Yang

et al. 2010

Biol Trace Elem Res

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“…Here, HC-F3 cells had a lower rate of proliferation than HC-F2 and were mainly in G0/G1. Gen- erally, cells in G0/G1 were smaller than cells in S/G2-M, and were eluted later by SdFFF [48]. Interestingly, HC-F1 cells, which predominantly expressed differentiation markers (K1, K10, involucrin) in correlation with the lowest proliferative activity, were mainly in S/G2-M (Fig.…”

Section: Analysis Of Dna Content By Flow Cytometrymentioning

confidence: 91%

“…As it had been shown that SdFFF could separate cells according to cell cycle distribution [48], we determined whether or not fractionation was correlated with cell cycle distribution (Fig. 5).…”

Section: Analysis Of Dna Content By Flow Cytometrymentioning

confidence: 99%

“…Different aspects have been evaluated including (1) monitoring of biological events [40][41][42][43]; (2) cell sorting of specific subpopulations such as pre-apoptotic [43], or differentiated cells [41]; (3) kinetics of biological events using both the monitoring and cell separation capacities of SdFFF [44,45]; and (4) cell sorting of specific phenotypes from complex cancer cell populations such as neuroblastomas [46,47]. Recently, we also used SdFFF to better understand the possible link between the kinetics and the extent of a biological event, such as apoptosis, and cell status (cell cycle position) at the start of induction [48].…”

Section: Introductionmentioning

confidence: 99%

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Sedimentation field-flow fractionation separation of proliferative and differentiated subpopulations during Ca2+-induced differentiation in HaCaT cells

Micallef

Battu

Pinon

et al. 2010

Journal of Chromatography B

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“…Microfluidic separation devices may rely on different physical principles from the fieldflow fractionation amongst which one finds sedimentation by gravity, 11 magnetophoresis, 12,13 or the Dean-effect in curved channels. [1][2][3][4][14][15][16][17] The latter is the reason for particles of certain sizes to focus in an equilibrium position in the cross-section of a microfluidic spiral.…”

Section: Introductionmentioning

confidence: 99%

Simulation and experimental determination of the online separation of blood components with the help of microfluidic cascading spirals

et al. 2015

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Microfluidic spirals were used to successfully separate rare solid components from unpretreated human whole blood samples. The measured separation ratio of the spirals is the factor by which the concentration of the rare component is increased due to the Dean effect present in a flow profile in a curved duct. Different rates of dilution of the blood samples with a phosphate-buffered solution were investigated. The diameters of the spherical particles to separate ranged from 2 μm to 18 μm. It was found that diluting the blood to 20% is optimal leading to a separation ratio up to 1.97. Using two spirals continuously placed in a row led to an increase in separation efficacy in samples consisting of phosphate-buffered solution only from 1.86 to 3.79. Numerical investigations were carried out to display the flow profiles of Newtonian water samples and the shear-thinning blood samples in the cross-section of the experimentally handled channels. A macroscopic difference in velocity between the two rheologically different fluids could not be found. The macroscopic Dean flow is equally present and useful to help particles migrate to certain equilibrium positions in blood as well as lower viscous Newtonian fluids. The investigations highlight the potential for using highly concentrated, very heterogeneous, and non-Newtonian fluidic systems in known microsystems for screening applications.

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Analysis of relationship between cell cycle stage and apoptosis induction in K562 cells by sedimentation field-flow fractionation

Cited by 17 publications

References 69 publications

Effect of Dietary High Molybdenum on the Cell Cycle and Apoptosis of Kidney in Broilers

Effect of Dietary High Molybdenum on the Cell Cycle and Apoptosis of Kidney in Broilers

Sedimentation field-flow fractionation separation of proliferative and differentiated subpopulations during Ca2+-induced differentiation in HaCaT cells

Simulation and experimental determination of the online separation of blood components with the help of microfluidic cascading spirals

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