Analysis of Reparative Activity of Platelet Lysate: Effect on Cell Monolayer Recovery In Vitro and Skin Wound Healing In Vivo

Сергеева, Н. С.; Shanskii, Ya. D.; Свиридова, И. К.; Каралкин, П. А.; Кирсанова, В. А.; Ахмедова, С. А.; Каприн, А. Д.

doi:10.1007/s10517-016-3563-3

Cited by 13 publications

(15 citation statements)

References 14 publications

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“…24 Platelet lysate has been shown to have effects on cell monolayer recovery in vitro and in the setting of wound healing in vivo. 25 We speculated that functional factors that promote wound healing may be decreased during pregnancy due to internal structural changes of platelets because CSD prevalence is negatively correlated with post-CS platelet concentration. 26 Although a higher fibrinogen concentration is beneficial in vaginal delivery, it is a predictor for CSD in women undergoing CS.…”

Section: Discussionmentioning

confidence: 99%

The prevalence and risk predictors of cesarean scar defect at 6 weeks postpartum in Shanghai, China: A prospective cohort study

Pan

Zeng

et al. 2018

Acta Obstet Gynecol Scand

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Introduction:The aim of this study was to explore the prevalence and predictors of cesarean scar defect (CSD) at 6 weeks postpartum in Shanghai, China. Material and methods:Women scheduled to receive a cesarean section (CS) were recruited from a university hospital. Surgery-related factors, pregnancy complications, routine examinations, perioperative medications, and physical signs were collected and transvaginal ultrasonography was performed to assess the presence of a CSD at 6 weeks postpartum. Multivariate logistic regression was applied to identify the predictors for CSD. Results:A CSD was detected in 223 of 514 women (43.4%, 95% CI 39.1%-47.7%) by transvaginal ultrasonography. Of women with normal temperature (T < 37.5°C, CSD prevalence 33.9%, 95% CI 28.4%-39.5%), women with postpartum fever (T ≥ 38°C, CSD 44.1%, 95% CI 31.0%-57.1%), and women who were subfebrile in the postpartum (37.5 ≤ T ≤ 38.0°C, CSD 58.3%, 95% CI 50.9%-65.7%), the latter two had significantly increased risk for CSD (adjusted odds ratio [aOR] 2.7, 95% CI 1.3-5.2 and aOR 3.3, 95% CI 2.1-5.3, respectively). In comparison to single-dose antibiotic administration (CSD 49.0%, 95% CI 43.8%-54.3%), multi-dose antibiotic administration (CSD 31.1%, 95% CI 23.8%-38.3%) had a protective effect (aOR 0.4, 95% CI 0.3-0.7).Postpartum fever, intrapartum infection, emergency CS, and cervical dilation ≥ 3 cm were found to be the main predictors of multi-dose antibiotics management. Low platelet count postpartum (defined as ≤ 150 × 10 9 /L) and high fibrinogen pre-CS (defined as ≥ 4.5 g/L) increased CSD risk (aOR 2.0, 95% CI 1.1-3.6 and 1.7, 95% CI 1.1-2.5, respectively). Conclusions:The prevalence of CSD in the Chinese population is high enough to be a concern. Perioperative infection and hypercoagulability should be considered CSD predictors, and multi-dose antibiotics have a protective effect.

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Section: Discussionmentioning

confidence: 99%

The prevalence and risk predictors of cesarean scar defect at 6 weeks postpartum in Shanghai, China: A prospective cohort study

Pan

Zeng

et al. 2018

Acta Obstet Gynecol Scand

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“…If a microscopic tumor is suspended in dormancy by insufficient blood supply, this release of VEGF may constitute an "angiogenic switch" allowing escape from dormancy by stimulated angiogenesis [17,30] and VEGF levels have been correlated with disease progression [31]. In vitro and in vivo studies have demonstrated both improved wound healing and enhanced breast cancer cell colony formation by addition of platelet lysate [32,33,34]. The main effect of platelet activation on cancer seems to be to promote invasion and metastasis.…”

Section: The Immediate Responsementioning

confidence: 99%

The link between wound healing and escape from tumor dormancy

Dillekås

Straume

2019

Surgical Oncology

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Tumor dormancy is considered one of the major unsolved questions in cancer biology. Understanding the mechanisms responsible for maintaining and interrupting dormancy would be a major step towards preventing overt metastatic disease. Increasing evidence points to tissue trauma and subsequent wound healing as contributing events in escape from dormancy. In this review, we outline relevant aspects of the wound healing process, and relate this to mechanisms of tumor dormancy and metastatic progression. In addition to important findings in epidemiological and experimental studies, more direct evidence of such a link has recently been presented. These results can have major implications for treatment and prevention of cancer.

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“…В то же время аутологичная плазма, обогащенная лизатом тромбоцитов, не теряет стимулирующего влияния на процессы пролиферации и миграции клеток. В отдельных работах показано, что лизат тромбоцитов стимулирует миграцию мультипотентных мезенхимных стромальных клеток жировой ткани человека и обеспечивает репарацию дефекта монослоя фибробластов человека и мультипотентных мезенхимных стромальных клеток in vitro в сходных с эмбриональной телячьей сывороткой концентрациях (5−10%) [27,28]. Лизат тромбоцитов из богатой тромбоцитами плазмы крови крыс стимулирует заживление кожного дефекта у животных за счет ускорения эпителизации и формирования грануляционной ткани.…”

Section: обсуждение основного результата исследованияunclassified

“…Лизат тромбоцитов из богатой тромбоцитами плазмы крови крыс стимулирует заживление кожного дефекта у животных за счет ускорения эпителизации и формирования грануляционной ткани. Регенерирующие свойства лизата тромбоцитов in vivo усиливаются с увеличением его концентрации [27]. В работе G. Sandri и соавт.…”

Section: обсуждение основного результата исследованияunclassified

Autologous Plasma Enriched with Platelet Lysate for the Treatment of Idiopathic Age-Related Macular Degeneration: A Prospective Study

et al. 2018

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Background: Plasma enriched in growth factors is widely used in medical practice. However, the clinical efficacy of its application in the treatment of age-related retinal integrity violations is investigated insufficiently. Aims: The aim of the study was to evaluate the clinical efficacy of autologous plasma enriched with platelet lysate for treating age-related macular degeneration. Materials and methods: A three-port subtotal transconjunctival vitrectomy was performed and administration of the autologous plasma enriched with platelet lysate was indicated. Autologous plasma enriched with platelet lysate was received from the peripheral blood. We assessed visual acuity, intraocular pressure; conducted optical coherent tomography examination of the eye on the side of the pathological process. Results: The study demonstrated that the combination of a standard 3-port transconjunctival subtotal vitrectomy followed by tamponade of the gap using the autologous plasma enriched with platelet lysate with the injections of the autologous plasma enriched with platelet lysate in the area of pterygopalatine fossa on the side of the operated eyes statistically significantly promoted recovery of the visual acuity in the early postoperative period (15 days) and late period (90 days) if compared with patients who received only surgical treatment (p≤0.05). Use of the autologous plasma enriched with platelet lysate in the treatment increased the closing rate of the tearing of the retina in the macular region up to 62,5%, while only surgical treatment leads to the closure of the defect of the retina in 37.5% of cases. The study showed that autologous plasma enriched with platelet lysate contains cytokines, growth factors, and nitric oxide which are involved in the regeneration/reparation of the retina. Conclusions: Additional administration of the autologous plasma enriched with platelet lysate in the scheme of treatment patients with age-related macular degeneration is accelerating the closure of retinal tears of the eye and improves visual acuity.

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Analysis of Reparative Activity of Platelet Lysate: Effect on Cell Monolayer Recovery In Vitro and Skin Wound Healing In Vivo

Cited by 13 publications

References 14 publications

The prevalence and risk predictors of cesarean scar defect at 6 weeks postpartum in Shanghai, China: A prospective cohort study

The prevalence and risk predictors of cesarean scar defect at 6 weeks postpartum in Shanghai, China: A prospective cohort study

The link between wound healing and escape from tumor dormancy

Autologous Plasma Enriched with Platelet Lysate for the Treatment of Idiopathic Age-Related Macular Degeneration: A Prospective Study

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