Analysis of rhizosphere bacterial communities of tobacco resistant and non-resistant to bacterial wilt in different regions

Abstract

Tobacco bacterial wilt has seriously affected tobacco production. Ethyl methanesulfonate (EMS) induced tobacco bacterial wilt resistant mutants are important for the control of tobacco bacterial wilt. High-throughput sequencing technology was used to study the rhizosphere bacterial community assemblages of bacterial wilt resistant mutant tobacco rhizosphere soil (namely KS), bacterial wilt susceptible tobacco rhizosphere soil (namely GS) and bulk soil (namely BS) in Xuancheng, Huanxi, Yibin and Luzhou. Alpha analysis showed that the bacterial community diversity and richness of KS and GS in the four regions were not significantly different. However, analysis of intergroup variation in the top 15 bacterial communities in terms of abundance showed that the bacterial communities of KS and GS were significantly different from BS, respectively. In addition, pH, alkali-hydrolysable nitrogen (AN) and soil organic carbon (SOC) were positively correlated with the bacterial community of KS and negatively correlated with GS in the other three regions except Huanxi. Network analysis showed that the three soils in the four regions did not show a consistent pattern of network complexity. PICRUSt functional prediction analysis showed that the COG functions were similar in all samples. All colonies were involved in RNA processing and modification, chromatin structure and dynamics, etc. In conclusion, our experiments showed that rhizosphere bacterial communities of tobacco in different regions have different compositional patterns, which are strongly related to soil factors.

Deciphering Differences in Microbial Community Diversity between Clubroot-Diseased and Healthy Soils

“…The results indicated that the diseased soils in Youxian and Lichuan may have a significant effect on bacterial diversity. Taken together, the field site location had a large effect in determining microbial community diversity in the present study, which is similar to previous studies that illustrated the importance of spatial influences [47,54,55].…”

“…It is noteworthy that the soil pH values of Lichuan and Jianshi were lower than those of Licang and Youxian, while the corresponding bacterial Chao1 indices of healthy and diseased soils in the two regions were, overall, at low levels among the four regions. Thus, soil pH may be one of the factors affecting bacterial diversity, which is in line with previous studies [ 47 , 48 ]. A high calcium concentration can reduce the rate of maturation of P. brassicae in root hairs and clubroot severity in Brassica crops [ 49 , 50 ].…”

Microbial interactions and metabolisms in response to bacterial wilt and black shank pathogens in the tobacco rhizosphere

“…TBW is a bacterial disease, and TBS is a fungal disease, which usually results in severe losses of yield and quality of tobacco, so that TBW and TBS can be excellent model for biocontrol research. The first task is to find effectively broad-spectrum microorganisms ( Zheng et al., 2021 ; Shi et al., 2022 ). In this study, we studied the response of rhizosphere soil microbial communities to these two plant diseases.…”

Insight into the soil bacterial community succession of Nicotiana benthamiana in response to Tobacco mosaic virus

“…The commonly used methods mainly include chemical pesticides and transgenic approaches, which cannot eliminate TMV load in infected tobacco plants and whose efficacy is still limited in the field. Microbes (e.g., Streptomyces , Pseudomonas , and Bacillus ) have been considered potential alternatives for chemical pesticides to inhibit plant pathogens, including fungal, bacterial, and viral pathogens ( Kim et al, 2022 ; Ren et al, 2022 ; Shi et al, 2022 ). Pseudomonas fluorescens could inhibit the activity of Cucumber mosaic virus , Tomato mottle virus , and Tobacco necrosis virus ( Maurhofer et al, 1998 ; Murphy et al, 2000 ).…”