Analysis of S-Allele Genotypes and Genetic Diversity in the Apple

Komori, Sadao; Soejima, J.; Abe, Katsuaki; Kotoda, Nobuhiro; Kato, Hazuki

doi:10.17660/actahortic.2000.538.9

Cited by 12 publications

(4 citation statements)

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“…Molecular S-genotyping of apple cultivars was initiated by Sassa et al (1994) using two-dimensional polyacrylamide gel electrophoresis analyses for the identification of 6 S-alleles labelled by alphabetical codes (S a -S f ). This was later extended by three additional alleles (S g -S i ) (Komori et al 2000). Then, S-genotypes of the 14 diploid cultivars proposed in the classic work of Kobel et al (1939) were confirmed by Bošković and Tobutt (1999) using non-equilibrium pH gel electrofocusing and isoelectric focusing.…”

Section: Communicated By E Dirlewangermentioning

confidence: 87%

S-genotyping of old apple cultivars from the Carpathian basin: methodological, breeding and evolutionary aspects

Halász

Hegedűs

György

et al. 2011

Tree Genetics & Genomes

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Apple exhibits self-incompatibility controlled by the multiallelic S-locus. Twenty-three old apple cultivars were S-genotyped using three different approaches (allele-specific polymerase chain reaction (PCR) + cleaved amplified polymorphic sequences (CAPS), consensus PCR + sequencing and consensus PCR+CAPS) to compare the robustness and reliability of these techniques and characterise genotypes from the Carpathian basin that might be useful in resistance breeding. Best results were obtained using the ASPF3 and ASPR3S consensus primer pair that detected 96% of all alleles carried by the 23 cultivars tested. Flow cytometry analysis was also needed to control the completeness of the genotypes as was seen in case of a tetraploid cultivar with only three assigned S-alleles. The genetic disparity between the old Carpathian basin and modern apple cultivars was indicated by differences in allele frequency data (S 9 , S 24 and S 26 ) as well as single nucleotide polymorphisms in S 1 , S 2 , S 7 S 24 and S 26 and indels in S 20 and S 26 alleles. An alignment of partial genomic sequences indicated trans-specific and transgeneric evolution of S-ribonuclease alleles in the Maloideae subfamily (S 26 and S 28 ) and a possibly recent introgression event (S 1 ) between Malus×domestica and Malus sylvestris. These data suggest that the genome of old cultivars from the Carpathian basin was enriched by several Malus taxa and are free from the consequences of modern breeding. These cultivars may contribute to the widening of the genetic basis of cultivated apple and prevent genetic erosion in future commercial cultivars.

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Section: Communicated By E Dirlewangermentioning

confidence: 87%

S-genotyping of old apple cultivars from the Carpathian basin: methodological, breeding and evolutionary aspects

Halász

Hegedűs

György

et al. 2011

Tree Genetics & Genomes

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“…The S-RNases (S1 to S29) and S-genotypes of 300 apple cultivars (Malus × domestica) were determined by pollination test (Kobel et al, 1939;Komori et al 1999Komori et al , 2000, protein analyses (Sassa et al 1994(Sassa et al , 1996 and DNA analysis (sequencing, S-allele specific PCR and PCR-CAPS; Broothaerts et al 1995;Janssens et al 1995;Sassa et al 1996;Verdoodt et al 1998;Matsumoto et al 1999aMatsumoto et al , b, 2000Matsumoto et al , 2001aSchneider et al 2001;Van Nerum et al 2001;Kitahara and Matsumoto 2002a, b;Matityahu et al 2005;Kim et al 2006;Gu et al 2015). However, the S30-RNase was designed from St-RNase of Malus transitoria .…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization ofS-RNasegenes in apple rootstock and the diversity ofS-RNasesinMalusspecies

et al. 2016

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We isolated and confirmed two S-RNases, denoted as mpS1 and mpS2, from apple rootstock 'Marubakaido' (Malus prunifolia Borkh. Var. ringo Asami). These S-RNases contained and conserved five cysteine residues and two histidine residues, which are essential for RNase activity. The mpS1 showed high similarity to S5 (99.1%) of Malus spectabilis, whereas the mpS2 showed 99.5% nucleotide sequence similarity to S26 of (Malus × domestica) and 99.6% to S35 of (Malus sieversii) when compared with reported S-RNases. In amino acid sequences, the mpS1-RNase was almost similar to the S5-RNase of Malus spectabilis, and the mpS2-RNase was similar to the S35 of Malus sieversii, with only one bp being different from the S26-RNase of Malus × domestica. The 57 S-RNases of Malus species were renamed and rearranged containing the new S-RNases, as mprpS35 (mpS2) and mprpS57 (mpS1), for determining S-genotypes and identifying new alleles from apple species (Malus spp.).

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“…As an alternative method, microscopic evaluation of pollen tube growth through the pistil has revealed 11 different S-alleles (S1 to S11) and assignment of alleles for 14 diploid and 12 triploid varieties (Kobel et al, 1939). Likewise, 10 S-alleles were identified among Japanese apple cultivars by Komori et al (1999Komori et al ( , 2000, who assigned a letter symbol S a to S i and S z and reported their correspondence to four of Kobel's S-alleles. As biochemical and molecular methods were developed, Sassa et al (1996) discriminated S-alleles (S a to S f ) through characteristic migration patterns of gene products in the alkaline regions of isoelectric focusing (IEF) or 2 dimensional polyacrylamid gel electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

Distribution of S-alleles among Korean Apples by PCR and Cross-pollination

Heo¹,

Kwon²,

Hwang³

et al. 2012

Korean Journal of Horticultural Science and Technology

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To acquaint correct information about the fertilizability and analyze S-allele based genetic diversity among Korean apples, we investigated self-incompatibility genotypes by PCR and cross-pollination tests in field. As a consequence, S-genotypes of Korean apples were distributed within narrow genetic diversity as S1S3 for 'Hongro' and 'Saenara'; S1S9 for 'Gamhong' and 'Manbok'; S3S5 for 'Seokwang'; S3S9 for 'Sunhong', 'Seohong', 'Chukwang', and 'Hwahong'. Coupled with cross-pollination experiments in field, our results provide support for the view that apples are fully compatible when both of their S-loci differ and semi-compatible when they carry one different and one identical S-locus. Furthermore, the results of this study indicate that S-alleles have to be extended to various genotypes for Korean apple breeding.

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Analysis of S-Allele Genotypes and Genetic Diversity in the Apple

Cited by 12 publications

References 0 publications

S-genotyping of old apple cultivars from the Carpathian basin: methodological, breeding and evolutionary aspects

S-genotyping of old apple cultivars from the Carpathian basin: methodological, breeding and evolutionary aspects

Identification and characterization ofS-RNasegenes in apple rootstock and the diversity ofS-RNasesinMalusspecies

Distribution of S-alleles among Korean Apples by PCR and Cross-pollination

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