Analysis of Saccharomyces cerevisiae proteins induced by peroxide and superoxide stress

Abstract

Exponentially growing

Bcl-2 family members inhibit oxidative stress-induced programmed cell death in Saccharomyces cerevisiae

“…S. cerevisiae is sensitive both to H 2 O 2 and to superoxide-generating agents [9][10][11]. Yeast deletion mutants of antioxidant genes such as catalase, superoxide dismutase, and cytochrome c peroxidase result in strains hypersensitive to lethal effects of oxidative stresses relative to isogenic wild-type cells [1,5,12].…”

Accumulation of small heat shock proteins, including mitochondrial HSP22, induced by oxidative stress and adaptive response in tomato cells

“…In bacteria, partial overlapping of OS and heat shock (HS) responses has been observed (Storz et al, 1990). Oxidative agents also induce the synthesis of HSP in cyanobacteria (Mittler and Tel-Or, 1991), yeast (Jamieson et al, 1994), Drosophila cells (Courgeon et al, 1990) and mammalian cells (Storz and Polla, 1996). Reports on plants are generally not conclusive and concern the induction by UV-B radiation of 'heat shock-like proteins' in Vigna sinensis seedlings (Nedunchezhian et al, 1992), the accumulation by gamma irradiation of putative small HSP (smHSP) in tomato fruits (Ferullo et al, 1994), and the very recent characterization of a parsley smHSP gene induced by ozone (Eckey-Kaltenbach et al, 1997).…”

Amino acid‐dependent regulation of the Saccharomyces cerevisiaeGSH1 gene by hydrogen peroxide

“…Despite the H 2 O 2 induction of all three genes being dependent on the presence of the Yap1 protein, it is clear that the mechanism of regulation of GSH1 by H 2 O 2 must differ from that used for both TRX2 and SSA1. In these cases, the Yap1 protein directly regulates TRX2 expression by H 2 O 2 , while the induction of SSA1 by H 2 O 2 is mediated via a STRE (Jamieson et al, 1994;Stephen et al, 1995;Mager and De Kruijff, 1995). The factor(s) that bind to the STRE and bring about expression in response to H 2 O 2 have not yet been identified, although it is evident that multiple factors must be involved in regulating gene expression in response to different stresses through the same element (Mager and De Kruijff, 1995;Martínez-Pastor et al, 1996;Schmitt and McEntee, 1996).…”

“…The results shown represent the mean Ϯ standard deviation of assays from three independent cultures (n = 3). SSA1 (Kuge and Jones, 1994;Jamieson et al, 1994;Stephen et al, 1995). Given the effect of casamino acids and the amino acids glutamate, glutamine and lysine on the induction of GSH1 by H 2 O 2 , we were interested in determining whether amino acids had any effect on the H 2 O 2 -mediated induction of these other genes.…”

“…The molecular details of oxidant adaptive stress responses have been characterized most extensively in Escherichia coli (Demple, 1991;Farr and Kogoma, 1991;Storz and Toledano, 1994). In yeast, at least part of the regulation of the oxidative stress responses is mediated at the level of transcription (Collinson and Dawes, 1992;Jamieson et al, 1994;Stephen et al, 1995). We are interested in understanding the mechanisms responsible for sensing oxidative stress and for ultimately regulating gene expression in response to stress.…”