Analysis of salivary phenotypes of generalized aggressive and chronic periodontitis through nuclear magnetic resonance‐based metabolomics

Romano, Federica; Meoni, Gaia; Manavella, Valeria; Baima, Giacomo; Tenori, Leonardo; Cacciatore, Stefano; Aimetti, Mario

doi:10.1002/jper.18-0097

Cited by 49 publications

(42 citation statements)

References 46 publications

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“…These include the small amount of sample needed compared with other volume average techniques, as well as the swiftness of the experimental data acquisition that can be performed in as fast as a few minutes [21]. The amount of saliva supernatant used for NMR analysis ranged from 0.17 to 0.60 mL (Table 1A) [3][4][5][6][7][8][9][10][11][12][13][14][15]. A saliva sample does not need any complex pre-treatment or preparation for the solution state NMR analysis to be performed [17].…”

Section: The Role Of Nuclear Magnetic Resonance In Saliva Testing Andmentioning

confidence: 99%

“…A saliva sample does not need any complex pre-treatment or preparation for the solution state NMR analysis to be performed [17]. Specifically, the NMR saliva sample preparation consists of centrifugation, and subsequent addition of a NMR solvent, standard, and buffer to the supernatant inside an NMR tube [3][4][5][6][7][8][9][10][11][12][13][14]. On the contrary, the more popular mass spectroscopic analysis requires multiple and more complex sample preparation steps (e.g., extraction, purification, digestion, separation), as well as costly labelling reference compounds for data acquisition and analysis.…”

Section: The Role Of Nuclear Magnetic Resonance In Saliva Testing Andmentioning

confidence: 99%

“…NMR on saliva was found to be able to distinguish with satisfactory predictability between healthy and active disease samples. Hence, the establishment of a metabolomic profile for periodontal disease is being attempted (Table 1B) [9,10]. However, the metabolic analysis of the patients did not distinguish between the two different types of periodontal disease [9].…”

Section: Periodontal Diseasementioning

confidence: 99%

“…Regarding the saliva collection methodology, certain recommendations have been proposed in the literature in order to minimize the influence of other factors and external variants, as well as to increase reproducibility. Saliva collection has been proposed to be performed 24 h after dental treatment to avoid blood contamination, and 12 h after the use of alcohol, nicotine, caffeine, or medications (if medically feasible) [9,17]. Morning collection is widely preferred in order to minimize the effect of the circadian rhythm.…”

Section: Proposed Saliva Collection Timing Protocol In the Dental CLImentioning

confidence: 99%

“…Magnetic resonance analysis of saliva has identified significant biomarkers correlated with oral, dental, and periodontal pathology, as well as salivary biomarkers linked to the presence of head and neck squamous cell carcinoma and sarcoidosis. Interestingly, salivary biomarkers have been also used in order to verify and monitor established health after therapy of the pathology [3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: Introduction-saliva Testingmentioning

confidence: 99%

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Current Status of Magnetic Resonance on Saliva for Oral and Dental Health Early Diagnosis

Katsavochristou

Koumoulis

2020

Magnetochemistry

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Magnetic resonance spectroscopy of biomolecules has recently gained attention for clinical diagnosis. Its combination with saliva collection and analysis can promote early disease detection and monitoring, by identifying biomarkers of specific underlying pathology or disease as detected in saliva. With this novel, non-invasive technique, certain salivary biomarkers have been linked to dental and periodontal tissues pathology, as well as to specific head and neck cancer malignancies. At present, diagnostic biomarkers are still in need for further identification (e.g., diagnosis and monitoring of Sjögren's syndrome), and nuclear magnetic resonance spectroscopy has been found to be a promising technique to compliment the current analytic methodology. Moreover, this article reports on the various data collection and analysis parameters used in the literature. Protocol standardization is yet to be established not only for the laboratory procedures, but also for the clinical sample collection. Herein, we review the current status of utilizing nuclear magnetic resonance in order to further support data on health associated biomarkers, and we also propose a saliva sampling scheduling protocol with the potential to be used in the clinical and experimental setting for standardization of the testing methodology.

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Section: The Role Of Nuclear Magnetic Resonance In Saliva Testing Andmentioning

confidence: 99%

Section: The Role Of Nuclear Magnetic Resonance In Saliva Testing Andmentioning

confidence: 99%

Section: Periodontal Diseasementioning

confidence: 99%

Section: Proposed Saliva Collection Timing Protocol In the Dental CLImentioning

confidence: 99%

Section: Introduction-saliva Testingmentioning

confidence: 99%

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Current Status of Magnetic Resonance on Saliva for Oral and Dental Health Early Diagnosis

Katsavochristou

Koumoulis

2020

Magnetochemistry

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Hochdurchsatz‐Metabolomik mit 1D‐NMR

et al. 2018

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Metabolomik befasst sich mit der Gesamtheit aller Metabolite, dem Metabolom. Als eine der “Omics”‐Wissenschaften hat sie Querbeziehungen zur Biologie, Physiologie, Pathologie und Medizin; andererseits sind Metabolite chemische Verbindungen, kleine organische Moleküle oder anorganische Ionen. Ihre korrekte Identifizierung und Quantifizierung in komplexen biologischen Matrizen erfordert daher eine solide chemische Grundlage. Im Vergleich beispielsweise zu DNA unterliegen Metabolite sehr viel stärker der Oxidation oder dem enzymatischen Abbau: Wir können große Teile eines Mammut‐Genoms aus einer kleinen Probe rekonstruieren, sind aber nicht in der Lage, dasselbe mit seinem Metabolom zu tun, das innerhalb weniger Stunden nach dem Tod des Tieres wahrscheinlich weitgehend abgebaut war. Erforderlich sind daher Standardverfahren, gute chemische Fertigkeiten der Probenvorbereitung für die Lagerung und anschließende Analyse, genaue analytische Protokolle, eine umfangreiche Kenntnis der Chemometrie, erweiterte statistische Verfahren und fundiertes Wissen über mindestens eine der beiden Metabolomik‐typischen Verfahren, MS oder NMR. All diese Fertigkeiten besitzen traditionell die Chemiker. Entsprechend betrachten wir die Metabolomik aus chemischer Perspektive und beschränken uns auf NMR. Der Analytiker findet Argumente für und gegen NMR, jedoch bietet das Verfahren eine besondere ganzheitliche Perspektive, die für künftige Anwendungen als eine bevölkerungsweite Screeningtechnik für Reihenuntersuchungen sprechen.

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Towards multiomic analysis of oral mucosal pathologies

et al. 2023

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Oral mucosal pathologies comprise an array of diseases with worldwide prevalence and medical relevance. Affecting a confined space with crucial physiological and social functions, oral pathologies can be mutilating and drastically reduce quality of life. Despite their relevance, treatment for these diseases is often far from curative and remains vastly understudied. While multiple factors are involved in the pathogenesis of oral mucosal pathologies, the host’s immune system plays a major role in the development, maintenance, and resolution of these diseases. Consequently, a precise understanding of immunological mechanisms implicated in oral mucosal pathologies is critical (1) to identify accurate, mechanistic biomarkers of clinical outcomes; (2) to develop targeted immunotherapeutic strategies; and (3) to individualize prevention and treatment approaches. Here, we review key elements of the immune system’s role in oral mucosal pathologies that hold promise to overcome limitations in current diagnostic and therapeutic approaches. We emphasize recent and ongoing multiomic and single-cell approaches that enable an integrative view of these pathophysiological processes and thereby provide unifying and clinically relevant biological signatures.

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Analysis of salivary phenotypes of generalized aggressive and chronic periodontitis through nuclear magnetic resonance‐based metabolomics

Abstract: Within the limitations of this study, CGP and GAgP metabolomic profiles were not unequivocally discriminated through a NMR-based spectroscopic analysis of saliva.

Cited by 49 publications

References 46 publications

Current Status of Magnetic Resonance on Saliva for Oral and Dental Health Early Diagnosis

Current Status of Magnetic Resonance on Saliva for Oral and Dental Health Early Diagnosis

Hochdurchsatz‐Metabolomik mit 1D‐NMR

Towards multiomic analysis of oral mucosal pathologies

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