Analysis of SARS-CoV-2 spike RBD binding to ACE2 and its inhibition by fungal cohaerin C using surface enhanced Raman spectroscopy

Abstract

The structure of the SARS-CoV-2 spike RBD and human ACE2 as well as changes in the structure due to binding activities were analysed using surface enhanced Raman spectroscopy. The inhibitor cohaerin C was applied to inhibit the binding between spike RBD and ACE2. Differences and changes in the Raman spectra were determined using deconvolution of the amide bands and principal component analysis. We thus demonstrate a fast and label-free analysis of the protein structures and the differentiation between bound and unbound states. The approach is suitable for sensing and screening and might be relevant to investigate other protein systems as well.

Molybdenum Disulfide-Assisted Spontaneous Formation of Multistacked Gold Nanoparticles for Deep Learning-Integrated Surface-Enhanced Raman Scattering

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Analysis and differentiation of toxic and non-toxic cyanobacteria using Raman spectroscopy

“…The Raman measurements were done using an in-house Raman setup which is similar to previous works. 12,18 Samples were prepared on glass slides and covered with cover glass. Afterwards, the cells were bleached using the Raman excitation laser and a 430 nm LED.…”

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Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection

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