Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma

Watson, Jennifer; Rice, Photini F. S.; Marion, Samuel L.; Brewer, Molly; Davis, John R.; Rodríguez, Jeffrey J.; Utzinger, Urs; Hoyer, Patricia B.; Barton, Jennifer K.

doi:10.1117/1.jbo.17.7.076002

Cited by 28 publications

(39 citation statements)

References 56 publications

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“…This is the first time these changes have been tracked in vivo and even with limited sample size the changes seen in ovarian microstructure are supported by results from prior ex vivo work. 24,25 For diagnosis seen in both studies (normal, atrophic, and tubular hyperplasia/tubular adenoma) the OCT and MPM images were qualitatively similar and quantitative analysis shows similar trends. Further, multiple time point imaging allowed us to determine the time scale for microscopic changes associated with disease development.…”

Section: Discussionmentioning

confidence: 60%

In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis

Watson

Marion

Rice

et al. 2013

Cancer Biology & Therapy

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Section: Discussionmentioning

confidence: 60%

In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis

Watson

Marion

Rice

et al. 2013

Cancer Biology & Therapy

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“…We have previously shown that a variety of quantitative image analysis techniques can be useful for identifying diseased tissues. 16,43 In particular, analysis of the spatial frequency spectrum and gray-level co-occurrence matrix parameters of OCT and SHG images has shown promise for automated, quantitative discrimination between normal and abnormal tissues. These techniques will be applied to VHIS images to assess their utility as quantitative methods discerning normal tissue from ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, SHG of collagen and THG of cell nuclei can provide tissue and cell morphological information in vivo that would otherwise only be accessible ex vivo by traditional hematoxylin and eosin (H&E) staining of fixed histological tissue sections. [15][16][17]23,24 Although promising for visualizing early changes indicative of cancer, multiphoton imaging methods rely on raster scanning and require a complex laser source. Alternatively, multiplexed volume holographic imaging, which can be realized in a mechanically simple, robust, and inexpensive system, allows for imaging of multiple depths simultaneously.…”

Section: Introductionmentioning

confidence: 99%

“…a developing neoplasm that would otherwise be missed by a surgeon making the critical decision to either remove or leave the ovaries. To optically visualize changes in surface epithelium and the subsurface stroma in carcinomas, several optical imaging modalities have been investigated, including optical coherence tomography (OCT), [9][10][11] confocal microscopy, 12 multiphoton microscopy, [12][13][14][15][16][17] and reflectance spectroscopy and microscopy. [18][19][20][21][22] OCT provides relatively large imaging depth and high temporal resolution, however it requires scanning and image reconstruction, adding to the complexity and cost of the instrument.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

et al. 2014

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Abstract. Ovarian cancer is the most deadly gynecologic cancer, a fact which is attributable to poor early detection and survival once the disease has reached advanced stages. Intraoperative laparoscopic volume holographic imaging has the potential to provide simultaneous visualization of surface and subsurface structures in ovarian tissues for improved assessment of developing ovarian cancer. In this ex vivo ovarian tissue study, we assembled a benchtop volume holographic imaging system (VHIS) to characterize the microarchitecture of 78 normal and 40 abnormal tissue specimens derived from ovarian, fallopian tube, uterine, and peritoneal tissues, collected from 26 patients aged 22 to 73 undergoing bilateral salpingo-oophorectomy, hysterectomy with bilateral salpingo-oophorectomy, or abdominal cytoreductive surgery. All tissues were successfully imaged with the VHIS in both reflectance-and fluorescence-modes revealing morphological features which can be used to distinguish between normal, benign abnormalities, and cancerous tissues. We present the development and successful application of VHIS for imaging human ovarian tissue. Comparison of VHIS images with corresponding histopathology allowed for qualitatively distinguishing microstructural features unique to the studied tissue type and disease state. These results motivate the development of a laparoscopic VHIS for evaluating the surface and subsurface morphological alterations in ovarian cancer pathogenesis. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…21 Second harmonic generation facilitates label-free imaging and has the potential to augment current excisional biopsy protocols for melanoma diagnosis and treatment. 28 Strupler et al 29 33 The cancerous and noncancerous tissues could be differentiated with 81.2% sensitivity and 77.8% sensitivity respectively. Hence, they recommended the usage of SHG to visualize collagen structure for early cancer detection.…”

Section: Preference Of Shg Over Compound Microscopementioning

confidence: 99%

Tumor-associated Collagen Signatures: An Insight

Pavithra¹

2017

World Journal of Dentistry

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Tumor microenvironment has a diverse capability to induce both beneficial and adverse consequences for tumorigenesis. It is a multifactorial process induced by the imbalance in the tumor cells and extracellular matrix (ECM). Collagen, the main component of ECM, is traditionally regarded as a passive barrier to resist tumor cell invasion. In recent years, collagen is marked to have its pivotal role to initiate and promote tumor progression. Remodeling of collagen has been appreciated in various benign and malignant tumors. These alterations can be identified and demonstrated as tumor-associated collagen signatures that can be demonstrated using second harmonic generation imaging. Recognition of these characteristic changes in the organization of collagen fiber may potentially serve as an early diagnostic marker in various pathological processes, such as hyperplastic, dysplastic, and cancerous tissues. This review focuses on the physiological and pathological orientation of collagen fibers in relation to epithelium that acts as an image-based biomarker.

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Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma

Cited by 28 publications

References 56 publications

In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis

In vivo time-serial multi-modality optical imaging in a mouse model of ovarian tumorigenesis

Simultaneous multiplane imaging of human ovarian cancer by volume holographic imaging

Tumor-associated Collagen Signatures: An Insight

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