Analysis of secreted peptidome from omental adipose tissue in polycystic ovarian syndrome patients

Jia, Genmei; Tao, Hong-Jiang; Xue, Yunping; Xu, Sujuan; Xue, Kai; Zhu, Qi; Chen, Xiaoyan; Liu, Xiaoguang; Xu, Siliang; Li, Qian; Xu, Pengfei

doi:10.1002/jcp.26393

Cited by 8 publications

(11 citation statements)

References 65 publications

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“…The visible blood vessels and connective tissue were removed from the tissue. After rinsed with PBS, the skeletal muscle tissues were cut into small pieces (3–4 mm 3 ) with scissors as described by an established protocol (36, 37). Tissue cutting will lead to release of damaged cells slowly into the medium.…”

Section: Methodsmentioning

confidence: 99%

“…The protein concentrations of supernatant from cultured skeletal muscle samples were detected by Bradford method (43) and integrity of these samples were evaluated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) combined with silver staining. Afterwards the peptides were separated from samples by treatment with Amicon ® Ultra Centrifugal Filters in 10-kDa (Merck Millipore, Billerica, MA, USA) according to the manufacturer's instruction as previously described (37). The “peptidome” present in the filtrates was desalted using a Strata X C18 column (Phenomenex, Torrance, CA, USA) and the desalted peptide solution was vacuum-dried with centrifuges for speed vacuum (SCAN SPEED 40, LaboGene) as previously described (44) and immediately frozen at −80°C until the following processing.…”

Section: Methodsmentioning

confidence: 99%

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A Comparative Peptidomic Characterization of Cultured Skeletal Muscle Tissues Derived From db/db Mice

Han

Wang

et al. 2019

Front. Endocrinol.

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As an important secretory organ, skeletal muscle has drawn attention as a potential target tissue for type 2 diabetic mellitus (T2DM). Recent peptidomics approaches have been applied to identify secreted peptides with potential bioactive. However, comprehensive analysis of the secreted peptides from skeletal muscle tissues of db/db mice and elucidation of their possible roles in insulin resistance remains poorly characterized. Here, we adopted a label-free discovery using liquid chromatography tandem mass spectrometry (LC-MS/MS) technology and identified 63 peptides (42 up-regulated peptides and 21 down-regulated peptides) differentially secreted from cultured skeletal muscle tissues of db/db mice. Analysis of relative molecular mass (Mr), isoelectric point (pI) and distribution of Mr vs pI of differentially secreted peptides presented the general feature. Furthermore, Gene ontology (GO) and pathway analyses for the parent proteins made a comprehensive functional assessment of these differential peptides, indicating the enrichment in glycolysis/gluconeogenesis and striated muscle contraction processes. Intercellular location analysis pointed out most precursor proteins of peptides were cytoplasmic or cytoskeletal. Additionally, cleavage site analysis revealed that Lysine (N-terminal)-Alanine (C-terminal) and Lysine (N-terminal)-Leucine (C-terminal) represents the preferred cleavage sites for identified peptides and proceeding peptides respectively. Mapped to the precursors' sequences, most identified peptides were observed cleaved from creatine kinase m-type (KCRM) and fructose-bisphosphate aldolase A (Aldo A). Based on UniProt and Pfam database for specific domain structure or motif, 44 peptides out of total were positioned in the functional motif or domain from their parent proteins. Using C2C12 myotubes as cell model in vitro, we found several candidate peptides displayed promotive or inhibitory effects on insulin and mitochondrial-related pathways by an autocrine manner. Taken together, this study will encourage us to investigate the biologic functions and the potential regulatory mechanism of these secreted peptides from skeletal muscle tissues, thus representing a promising strategy to treat insulin resistance as well as the associated metabolic disorders.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Comparative Peptidomic Characterization of Cultured Skeletal Muscle Tissues Derived From db/db Mice

Han

Wang

et al. 2019

Front. Endocrinol.

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“…In recent years, with the technical development of proteomics and related methodologies, endogenous peptides are going to become a promising research focus, attracting the attention of a large number of researchers. 21 A number of researches have been performed to identify the endogenous peptides which participated in adiposity, 32 preeclampsia, 33 bronchopulmonary dysplasia, 31 polycystic ovarian syndrome 9 and, especially in cancer. 34 These studies further revealed the pathogenesis of corresponding diseases and provided new ideas and choices for the diagnosis and treatment of these diseases.…”

Section: Discussionmentioning

confidence: 99%

“…Peptide is a type of organic compound produced by endocrine glandular tissues or organs, containing 3-50 amino acids. 9 As an important role, peptide participates in almost all physiological activities, including cell differentiation, immune regulation and, especially, tumor formation. 10 In recent years, the application of peptides in the diagnosis and treatment of BC has aroused increasing interest.…”

Section: Introductionmentioning

confidence: 99%

<p>Profiling Analysis Reveals the Crucial Role of the Endogenous Peptides in Bladder Cancer Progression</p>

Zhang

et al. 2020

OTT

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Background: Peptide drugs provide promising regimes in bladder cancer. In order to identify potential bioactive peptides involved in bladder cancer, we performed the present study. Methods: Liquid chromatography/mass spectrometry assay was used to compare the endogenous peptides between bladder cancer and normal control. The potential biological functions of these dysregulated peptides are assessed by GO analysis and KEGG pathway analysis of their precursors. The SMART and UniProt databases are used to identify the sequences of the dysregulated peptides located in the functional domains. The Open Targets Platform database was used to investigate the precursors related to metabolic diseases. Results: A total of 9 up-regulated peptides and 110 down-regulated peptides in bladder cancer compared with normal control were identified (fold change > 1.2, P < 0.05). The MW of these dysregulated peptides ranged from 500 Da to 2500 Da and the MW of all identified peptides was below 3500 Da. The GO and KEGG pathway analysis indicated that these dysregulated peptides could play an important role in bladder cancer. Our further analysis revealed that 45 HFNPRFNAHGDAN 57 derived from LGALS1 and those peptides derived from P4HB and SERPINA1 might be the promising diagnostic biomarkers and therapeutic targets of bladder cancer. Conclusion: In the present study, we have identified the profile of the peptides significantly dysregulated in bladder cancer. Moreover, using bioinformatic analysis, we found the peptides derived from LGALS1, P4HB and SERPINA1 could be the promising diagnostic biomarkers and therapeutic targets of bladder cancer.

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“…The peptides were extracted and labeled with tandem mass tag (TMT) according to the manufacturer's instructions. 19,20 The labeled samples were analyzed by the Analysis and Testing Center of Nanjing Medical University (http://fxcszx. njmu.edu.cn/).…”

Section: Lc-ms/ms Detectionmentioning

confidence: 99%

Peptidomics analysis of myometrium tissues in term labor compared with term nonlabor

Fan

Hou

Xing

et al. 2019

J of Cellular Biochemistry

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Preterm birth (PTB) is a major cause of neonatal mortality, with a poorly understood etiology. The regular contraction of the myometrium was considered as contributing to the etiology of the onset of labor, especially PTB. Thus, studying the mechanism of myometrium contraction is very important for understanding the initiation of labor and also for preventing PTB. Using liquid chromatography‐mass spectrometry, we found 322 significantly differential peptides in myometrium tissues between term nonlabor and term labor groups (absolute fold change ≥ 2 and P < .05). We next analyzed length, molecular weights, isoelectric point, and cleavage site of all the different peptides. We, next, analyzed the functions of different peptides through their precursor proteins by Gene Ontology, enrichment and canonical pathway analysis. The results indicated that the extracellular matrix (ECM) played a major role in biological process, the cellular component, and molecular function categories, and revealed that ECM remodeling played a vital role in myometrial contraction. In addition, some known signaling, such as corticotropin‐releasing hormone signaling and calcium signaling were proven to be involved in this process. Ingenuity Pathways Analysis upstream regulator analysis suggested that some of the known molecules, which reportedly were very important in labor onset, were included, for example, nuclear factor κB, tubulin, and phosphoinositide 3‐kinase. We also identified 23 peptides derived from the precursor protein TITIN, of which 21 peptides sequences from TITIN were located in functional domains. These results suggested that peptides play an important role in labor onset and provide further insight into PTB therapy.

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Analysis of secreted peptidome from omental adipose tissue in polycystic ovarian syndrome patients

Cited by 8 publications

References 65 publications

A Comparative Peptidomic Characterization of Cultured Skeletal Muscle Tissues Derived From db/db Mice

A Comparative Peptidomic Characterization of Cultured Skeletal Muscle Tissues Derived From db/db Mice

<p>Profiling Analysis Reveals the Crucial Role of the Endogenous Peptides in Bladder Cancer Progression</p>

Peptidomics analysis of myometrium tissues in term labor compared with term nonlabor

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