2022
DOI: 10.32604/phyton.2022.020065
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Analysis of Seed Phenotypic and Metabolic Characteristics of Diploid and Tetraploid Tartary Buckwheat

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“…Plants were grown in an experimental field and subjected to standard field management practices. Flow cytometry was used to analyze the DNA content in the leaf cells of diploid Tartary buckwheat and colchicine-induced materials to confirm that a stable tetraploid Tartary buckwheat material was obtained [27]. After about 20 days, the fifth leaf from the shoot apex of diploid and tetraploid Tartary buckwheat seedlings was sampled, flash frozen in liquid nitrogen, and stored at −80°C until use.…”
Section: Experimental Materialsmentioning
confidence: 99%
“…Plants were grown in an experimental field and subjected to standard field management practices. Flow cytometry was used to analyze the DNA content in the leaf cells of diploid Tartary buckwheat and colchicine-induced materials to confirm that a stable tetraploid Tartary buckwheat material was obtained [27]. After about 20 days, the fifth leaf from the shoot apex of diploid and tetraploid Tartary buckwheat seedlings was sampled, flash frozen in liquid nitrogen, and stored at −80°C until use.…”
Section: Experimental Materialsmentioning
confidence: 99%