Analysis of Selected Lymphocyte (CD45+) Subset Distribution in Capillary Blood of Young Soccer Players

Nowak, Robert; Kostrzewa-Nowak, Dorota; Buryta, Rafał

doi:10.1519/jsc.0000000000003105

Cited by 3 publications

(4 citation statements)

References 33 publications

(64 reference statements)

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“…The CD4 + FoxP3 + (Treg) cells are also involved in post-effort immune modulation because of their role as central anti-inflammatory regulators of the immune response [ 16 , 18 ]. The participation of T cells in the modulation of immunity during maximal effort has been described in athletes [ 19 , 20 , 21 , 22 ]. On the other hand, numerous physically active young men, even though they are not professional athletes, use professional training programs to improve their physical fitness.…”

Section: Introductionmentioning

confidence: 99%

Differential Th Cell-Related Immune Responses in Young Physically Active Men after an Endurance Effort

Kostrzewa-Nowak

Nowak

2020

JCM

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The participation of T cell subsets in the modulation of immunity in athletes triggered by maximal effort was investigated. In total, 80 physically active young men (range 16–20 years) were divided into 5 age groups: 16, 17, 18, 19, and 20 years old. They performed efficiency tests on mechanical treadmills until exhaustion. White blood cell (WBC) and lymphocyte (LYM) counts were determined, and the type 1 (Th1), type 2 (Th2) helper T cells, T helper 17 (Th17), and T regulatory (Treg) cell distribution and plasma levels of selected cytokines were analyzed. An increase in WBC and LYM counts after the test and in Th1 and Treg cells after the test and in recovery was observed. There were no changes in Th2 cells. An increase in interleukins (IL): IL-2 and IL-8 was observed. The IL-6 level was altered in all studied groups. IL-17A and interferon gamma (IFN-γ) levels were increased in all studied groups. The mechanism of differential T cell subset activation may be related to athletes’ age. The novel findings of this study are the involvement of Th17 cells in post-effort immune responses and the participation of IL-6 in post-effort and the long-term biological effect of endurance effort.

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Section: Introductionmentioning

confidence: 99%

Differential Th Cell-Related Immune Responses in Young Physically Active Men after an Endurance Effort

Kostrzewa-Nowak

Nowak

2020

JCM

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“…Although previous studies suggest that neutrophils are mobilized in response to exercise-induced muscle damage 5 , 24 , we did not observe significant changes in total WBC counts or in granulocyte distribution in subjects after post-match recovery. While changes in the post-match distributions of different WBC subsets (namely lymphocytes and monocytes) were observed among junior soccer players 45 , it was not confirmed during long-term observation among senior soccer players. This suggests that the adaptive mechanism and adequate recovery are important factors in immunobalance as represented by the WBC distribution in capillary blood.…”

Section: Discussionmentioning

confidence: 76%

“…From this point of view, a lack of significant changes after the recovery time might be related to the biological adaptation of the immune system to inflammation pathways involved in the post-effort immune response. It seems that the cell balance in capillary blood is disordered only in non-professional athletes and after inadequate recovery time 45 .…”

Section: Discussionmentioning

confidence: 99%

Post-match recovery profile of leukocyte cell subsets among professional soccer players

Kostrzewa-Nowak

Wityk

Ciechanowicz

et al. 2021

Sci Rep

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This study assessed the impact of cumulative match time on the distribution of CD45+ cell subtests in the capillary blood of professional soccer players. Twenty-two males (aged 18–30 years) took part in the 36-week study. Participants playing up to 540 in cumulative match time and less than 30 min in each single match during the observation period formed the control group. White blood cell (WBC) phenotyping and creatine kinase (CK) plasma activity analyses were performed. Also, counts for WBC subsets were determined. No significant differences in the hematological parameters or lymphocyte and NK cell percentages were observed between the control and study groups. Changes in the T cell percentage were significant during weeks 11 and 30 and in Th and Tc cell percentages during weeks 2 and 26. Significant correlations were found between the cumulative match time and Th, NK, and B cell percentages; monocyte counts; and CK activity in the control group. However, for the study group, correlations were found between cumulative match time and Th, Tc, and B cell percentages; CK activity; and the CK ratio. Our study suggests that the distribution of CD45+ cells might be a useful tool for monitoring the immune status of professional soccer players.

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“…The lower expression of WBC, LYM, and LYM% in the HL group compared with that of the HH group suggests that soldiers with higher altitude running speeds have lower levels of inflammatory stress and better antioxidant capacity (Xiong et al, 2020). Kostrzewa et al confirmed that the increase in WBC count after exercise is related to the immune response and is not solely caused by dehydration (Nowak et al, 2020). WBC counts often decline after prolonged training, and high altitudes are associated with an inflammatory response (Galun et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Identification of novel urine proteomic biomarkers for high stamina in high-altitude adaptation

Guo

Shen

et al. 2023

Front. Physiol.

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Introduction: We aimed to identify urine biomarkers for screening individuals with adaptability to high-altitude hypoxia with high stamina levels. Although most non-high-altitude natives experience rapid decline in physical ability when ascending to high altitudes, some individuals with high-altitude adaptability continue to maintain high endurance levels.Methods: We divided the study population into two groups: the LC group (low change in endurance from low to high altitude) and HC group (high change in endurance from low to high altitude). We performed blood biochemistry testing for individuals at high altitudes and sea level. We used urine peptidome profiling to compare the HH (high-altitude with high stamina) and HL (high-altitude with low stamina) groups and the LC and HC groups to identify urine biomarkers.Results: Routine blood tests revealed that the concentration of white blood cells, lymphocytes and platelets were significantly higher in the HH group than in the HL group. Urine peptidome profiling showed that the proteins ITIH1, PDCD1LG2, NME1-NME2, and CSPG4 were significantly differentially expressed between the HH and HL groups, which was tested using ELISA. Urine proteomic analysis showed that LRG1, NID1, VASN, GPX3, ACP2, and PRSS8 were urine proteomic biomarkers of high stamina during high-altitude adaptation.Conclusion: This study provides a novel approach for identifying potential biomarkers for screening individuals who can adapt to high altitudes with high stamina.

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Analysis of Selected Lymphocyte (CD45+) Subset Distribution in Capillary Blood of Young Soccer Players

Cited by 3 publications

References 33 publications

Differential Th Cell-Related Immune Responses in Young Physically Active Men after an Endurance Effort

Differential Th Cell-Related Immune Responses in Young Physically Active Men after an Endurance Effort

Post-match recovery profile of leukocyte cell subsets among professional soccer players

Identification of novel urine proteomic biomarkers for high stamina in high-altitude adaptation

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