Analysis of seminal plasma biochemical parameters and sperm cryostability in different age groups of stallions

Atroshchenko, Mikhail; Kudlaeva, Anna; Fomina, M A; Kalashnikov, V. V.; Zaitsev, A. B.; Denisova, O. V.; Navasardyants, D. G.; Belonovskaya, O. S.; Pasko, A. A.

doi:10.1088/1755-1315/341/1/012162

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Variations in semen quality between Poitou sires and within the same individual are expected (18). These differences might be influenced by individual variations or the animal age (44,45). Male donkeys reach puberty between 19 and 20 months of age where sperm in ejaculates can be observed, although at this age they have not completed their full sexual maturation (46).…”

Section: Discussionmentioning

confidence: 99%

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

et al. 2021

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The Baudet du Poitou is a vanishing donkey breed recognized for engendering robust working mules. In Chile, only two pure breed Poitou males exist, which belong to the Chilean army and are used for mule production. We performed an extensive sperm and seminal analysis of these two jackasses aged 3 and 6 years and investigated the use of a simple hypometabolic extender for sperm cryopreservation. Computer-assisted sperm analysis showed high motility, velocity, and linearity in sperm movement. The seminal plasma analysis revealed that sodium and chloride were the main electrolytes, and globulins were the main metabolites. Active and variable enzymatic activity was observed. New information is reported about gamma-glutamyltransferase, aspartate aminotransferase, zinc, and magnesium concentrations in seminal plasma of Poitou donkeys. Ejaculates among jackasses showed some variability due to individual variability and different stages in sexual maturation according to age. The freezability index analysis based in viability, total motility and progressive motility with Botucrio extender (57.1 ± 11.0%; 56.6 ± 20.0%; and 22.6 ± 10.3%, respectively) were significantly higher (p < 0.05, p < 0.0001, and p < 0.0001, respectively) than with HM-0 extender (42,6 ± 11.4%; 14.9 ± 5.1%; and 1.0 ± 2.5%, respectively). We report new information on Poitou donkey semen and cryopreservation in the Southern Hemisphere that could be useful in donkey breeding and conservation programs to develop strategies that improve the effectiveness of population management of this breed.

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Section: Discussionmentioning

confidence: 99%

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

et al. 2021

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“…The proportion of spermatozoa that survive after freezing-thawing is affected by their susceptibility to cold shock, cooling rate, diluent composition, and osmotic stress, and the functional state of spermatozoa after cryopreservation depend on the action of oxidative stress, membrane stability, membrane receptor integrity, and nuclear structure state (Watson 2000;Elkina et al 2011). Various biologically active compounds can also influence sperm quality and cryostability, among which steroid hormones play a special role (Atroshchenko et al 2019c;Martínez-Fresneda et al 2019;Coloma et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of Testosterone, Dihydrotestosterone, Estradiol and Cortisol on the Quality of Fresh and Cryopreserved Stallion Sperm

Shitikova

Atroshchenko

Krokhotina³

et al. 2022

J Exp Bio & Ag Sci

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The effect of steroid hormones on the quality of fresh and cryopreserve sperm has not been fully understood. This study aimed to evaluate the effect of testosterone, dihydrotestosterone, estradiol, and cortisol on the quality of fresh and cryopreserved stallion sperm. The study was conducted on 40 Equus caballus stallions, including Arab (n=20), Oryol trotting (n=4), Standardbred (n=4), and Soviet Heavy Draft (n=12) breeds. The average age of the experimental animals was 9.9 ± 0.7 years. We determined standard quality indicators in fresh and cryopreserved sperm and the concentration of steroid hormones in the blood plasma of stallions. Results of the study suggested a negative correlation between the level of testosterone with total (r=-0.41; p<0.01) and progressive (r=-0.44; p<0.01) sperm motility in cryopreserved sperm as well as in fresh sperm (r=-0.38; p<0.05 and r=-0.39; p<0.05 correspondingly). While the level of estradiol showed a positive correlation with survival rate in cryopreserved (r=0.35; p<0.05) and in fresh (r=0.33; p<0.05) sperm. Further, the level of cortisol in the blood plasma of stallions did not show any statistically significant correlations with the qualitative characteristics of sperm. A positive relationship was found between the concentration of dihydrotestosterone with the volume of ejaculate (r=0.37; p<0.05) and the total number of sperm in the ejaculate (r=0.43; p<0.01). Results of the study can be concluded that steroid hormones have different effects on the quality indicators of fresh and cryopreserved sperm of stallions and their concentration in the blood should be considered when selecting stallions for cryopreservation of sperm.

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“…Mammalian sperm plasma is a composite mixture of epididymal secretions and accessory gonads (La Falci et al, 2002). It creates the mandatory environment for the normal functioning of sperm, and the present amount and type of enzymes and metabolites strongly affects sperm quality and freezing stability (Asadpour, 2012;Juyena and Stelletta, 2012;Mráčková et al, 2015;Atroschenko et al, 2019). Some of the enzymes are found in sperm, and they play an important role in fertilization, metabolic processes and the conversion of chemical energy into mechanical, which ensures the movement of sperm (Baychev et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams

et al. 2022

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The current study aimed to examine the percentage changes of viability sperm and the activity of the enzymes LDH, ALP, GOT / AST, GPT / ALT in the sperm plasma of Lacaune rams, before and after cryopreservation. For this purpose, five rams were examined, and two ejaculates were obtained from each ram. Ejaculates are collected by the method of artificial vagina, during the insemination campaign. All ejaculates were diluted with a 6AG extender and frozen by the Cassou?s sequin method. Sperm viability was determined by eosin and nigrosine smears, and enzyme activity was examined spectrophotometrically. As a result, the percentage of vital sperm after cryopreservation decreased by 15% (P ? 0.001). The freezing and thawing process also reduced the activity of the enzymes LDH, ALP, GOT / AST and GPT / ALP. In conclusion, the observed enzymes, in relation to sperm vitality, could be used as indicators to optimize the protocols for cryopreservation of ram?s sperm.

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Analysis of seminal plasma biochemical parameters and sperm cryostability in different age groups of stallions

Cited by 5 publications

References 13 publications

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

Effect of Testosterone, Dihydrotestosterone, Estradiol and Cortisol on the Quality of Fresh and Cryopreserved Stallion Sperm

Analysis of vitality and biochemical parameters in freeze-thawed seminal plasma of rams

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