Analysis of serum homocysteine in the laboratory practice - comparison of the direct chemiluminescence immunoassay and high performance liquid chromatography coupled with fluorescent detection

Paprotny, Łukasz; Wianowska, Dorota; Izdebska, Magdalena; Celejewska, Agnieszka; Szewczak, Dorota; Solski, Janusz

doi:10.11613/bm.2020.030703

Cited by 18 publications

(12 citation statements)

References 24 publications

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“…Therefore, a new concept under the chemical reaction should be required to discriminate between Cys and Hcy within a single fluorophore molecule. Currently, the monitoring for Cys and Hcy within the biothiol mixture has necessitated intricate methodologies such as high-performance liquid chromatography MS/MS (HPLC-MS/MS), amino acid analysis, and electrochemical assessment. , Due to their complex processes, these methods are currently limited in their capability to enable rapid monitoring of Cys and Hcy. From a clinical perspective, the complex procedures interrupt precise measurements and result in high costs for patients.…”

Section: Introductionmentioning

confidence: 99%

Next-Generation Femtech: Urine-Based Cervical Cancer Diagnosis Using a Fluorescent Biothiol Probe with Controlled Smiles Rearrangement

An,

Jeong,

Jung

et al. 2024

ACS Appl. Mater. Interfaces

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Cervical cancer screening is a crucial field of femtech (female technology). In this work, we disclosed a new femtech solution�a simple, straightforward, and on-site applicable urine-based cervical cancer diagnostic method using a fluorescent biothiol probe. Our newly developed nitrobenzene-based fluorescent probe, named NPS-B, effectively differentiates between cysteine and homocysteine within urine samples via controlled Smiles rearrangement. The analysis of emissionbased signals offers the potential utility of this method in cervical cancer. NPS-B was designed by considering the substitution effect and structural polarity of the nitrobenzene-based fluorophore. This controlled modification of nitrobenzene-induced substantial intramolecular charge transfer changes in the fluorophore when exposed to biothiols, resulting in significant changes in photophysical properties. NPS-B displayed different emissions of cysteine and homocysteine in clinical human urine (without prior urine treatment). Overall, our findings provide insights not only into fundamental chemical science but also into the broader domain of applied sciences.

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Section: Introductionmentioning

confidence: 99%

Next-Generation Femtech: Urine-Based Cervical Cancer Diagnosis Using a Fluorescent Biothiol Probe with Controlled Smiles Rearrangement

An,

Jeong,

Jung

et al. 2024

ACS Appl. Mater. Interfaces

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“…Thus far, various analytical methods have been developed for hcy determination, including electrochemical, 14 colorimetric, 15 chromatography, 16 chemiluminescence analysis, 17 and liquid chromatography–mass spectrometry techniques 18 . However, these analytical methods have some limitations, such as complex operation, long analysis time, and large sample consumption.…”

Section: Introductionmentioning

confidence: 99%

Covalent organic framework film–based laser desorption/ionization mass spectrometry for rapid and sensitive quantification of homocysteine in human serum

Chen

Ouyang

et al. 2023

Rapid Comm Mass Spectrometry

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Rationale Homocysteine (hcy) is a metabolite in the human body and an important determinant of cardiovascular health. To assist in the assessment of human cardiovascular safety, a rapid and accurate quantitative analysis method must be developed. Laser desorption/ionization mass spectrometry (LDI‐MS) has been widely used in biological, chemical, and medical analyses due to its excellent characteristics of high throughput, low sample consumption, and high speed. Here, an LDI‐MS method based on covalent organic framework (COF) film was developed for the rapid and sensitive determination of hcy in human serum using an isotope‐labeled internal standard. Methods We tried to cultivate COFs on indium−tin oxide substrate at room temperature to form a thin film, which was used in LDI‐MS. Compared with the traditional organic matrices, the COF film had a clean background and a high signal response for the detection of hcy. In addition, using COF film as the substrate, with a high signal intensity and a clean background, can be helpful to analyze a series of small molecules such as amino acids, bisphenols (Bps), estrogens, and drugs. We evaluated the limit of detection (LOD) and the reproducibility of this method. Finally, the calibration curve was constructed for the quantification of serum hcy using an isotope‐labeled internal standard and was applied for the rapid determination of hcy in clinical human serum. Results COF film–assisted LDI‐MS had a higher response signal and a cleaner background compared to four conventional organic matrices. The LOD of the method for hcy was 0.5 μmol/L, equivalent to 500 fmol. This method exhibited excellent performance for small‐molecule compounds, including amino acids, Bps, drugs, and estrogens. The reproducibilities of this method for shot‐to‐shot and dot‐to‐dot were determined to be good. This method was applied for the rapid analysis of hcy in clinical human serum, showing good correlation with those obtained by hospital testing. Conclusion The COF film–based LDI‐MS method had simple sample preparation, short analysis time, clean background, and good reproducibility for hcy analysis. As an important indicator of human health, the detection of serum hcy is of great significance to human health.

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“…However, only the results published at the beginning of the 21st century, proving the relationship between senile diseases and vitamin K deficiency, fully reveal its importance [ 2 , 4 ]. Taking into account the main health problems of the modern world and the fact that aging of the population is one of the dominant trends in the 21st century, the most important biological properties are the prevention of osteoporosis and cardiovascular diseases as well as the reduction in the risk of cancer [ 3 , 4 , 5 , 6 , 7 ]. According to [ 2 , 3 , 4 , 5 , 6 , 8 ] these properties are more specific to MK-7, hence the growing interest in determining this compound is observed.…”

Section: Introductionmentioning

confidence: 99%

Development, Validation, and Two-Year Application of Rapid and Simple LC-MS/MS-Based Method for the Determination of K2MK-7 in Blood Samples

Paprotny,

Szewczak,

Bryshten

et al. 2023

Molecules

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Biological properties of menaquinone-7, one of the vitamin K2 vitamers (K2MK-7), both those proven and those that remain to be investigated, arouse extensive interest that goes beyond the strictly scientific framework. The most important of them is the prevention of age-related diseases, considering that we live in the times identified as the era of aging societies and many people are exposed to the vitamin K2MK-7 deficiency. Therefore, an effective analytical protocol that can be adopted as a diagnostic and preventive analytics tool is needed. Herein, a simple sample preparation method followed by the liquid chromatography-tandem mass spectrometry-based method (LC-MS/MS), was used for the selective and sensitive determination of K2MK-7 in serum samples. Under the optimized conditions, using 500 µL of serum and the same amount of n-hexane, the reproducibility and the accuracy were obtained in the ranges of 89–97% and 86–110%, respectively, and the limit of detection value was 0.01 ng/mL. This method was used for the routine analysis. Statistical interpretation of the data from 518 samples obtained during 2 years of practice allowed for obtaining information on the content and distribution of K2MK-7 in the Polish population, broken down by the sex and age groups.

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Analysis of serum homocysteine in the laboratory practice - comparison of the direct chemiluminescence immunoassay and high performance liquid chromatography coupled with fluorescent detection

Cited by 18 publications

References 24 publications

Next-Generation Femtech: Urine-Based Cervical Cancer Diagnosis Using a Fluorescent Biothiol Probe with Controlled Smiles Rearrangement

Next-Generation Femtech: Urine-Based Cervical Cancer Diagnosis Using a Fluorescent Biothiol Probe with Controlled Smiles Rearrangement

Covalent organic framework film–based laser desorption/ionization mass spectrometry for rapid and sensitive quantification of homocysteine in human serum

Development, Validation, and Two-Year Application of Rapid and Simple LC-MS/MS-Based Method for the Determination of K2MK-7 in Blood Samples

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