Analysis of sex ratio on bovine in vitro fertilized embryos using sex determination kit treated sperm

Heo, Young-Tae; Kim, Dong-Gu; Uhm, Sang Jun

doi:10.12750/jet.2018.33.3.169

Cited by 4 publications

(5 citation statements)

References 17 publications

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“…Barsuren et al (2019) reported that sex-controlled calves were produced without a decrease in pregnancy rate and no increase or decrease in duration of pregnancy when using WM not combined with MNPs. According to a previous report, it was possible to successful sperm sexing and control of embryos using WM which MNPs were not combined (Heo et al, 2018). With the use of WM, the concentration of sperm will be halved due to sex segregation, and it is assumed that the reduced concentration would not affect fertility of sperm or pregnancy during AI.…”

Section: Discussionmentioning

confidence: 99%

Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics

Hwangbo

Lee

et al. 2021

J Anim Reprod Biotechnol

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The immunological sperm separation method is economical compared to the existing sorting method, and it is promising for the development of new technologies by reducing sperm damage. Wholemom (WM) is a sex-regulating protein that comprises on immunoglobulin G coupled with magnetic nanoparticles (MNPs) that responds to surface proteins derived from the Y chromosome in cattle. Y sperms are restricted in motility as the WM aggregates them, and the magnet could separate the non-aggregated cells. This study was conducted to investigate the effect of WM treatment on the characteristics of bull sperm. After treating sperm with WM and incubation for 6 h, the motility parameters including total motility, progressive motility, velocity average path, velocity straight line, amplitude of lateral head displacement, and linearity were significantly higher in the WM treatment group than in the control group (p < 0.05). Sperm viability and acrosome reaction rates were similar in both groups during each incubation period (p > 0.05). In conclusion, the immunological sperm sexing procedure using a monoclonal antibody conjugated with MNPs did not affect the characteristics of bull sperm. This study suggests that compared to other techniques, the immunological method for sperm sexing could classify sperm quickly and efficiently without the use of expensive equipment.

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Section: Discussionmentioning

confidence: 99%

Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics

Hwangbo

Lee

et al. 2021

J Anim Reprod Biotechnol

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“…WholeMan, in turn, increases the motility of the Y-sperm, promoting male gestations, and is only available for cattle [ 90 ]. Both kits are easy to apply before artificial insemination and the accuracy ranges from 70–90% depending on the species [ 90 , 91 ]. There is limited literature available to substantiate the results disseminated by the company.…”

Section: Sperm Sexing Through Immunological Methods: a Promising Weaponmentioning

confidence: 99%

“…Nonetheless, according to a paper published in 2018, where cow oocytes were in vitro fertilized with frozen-thawed semen samples, treated or not with the WholeMom kit, the female ratio was significantly higher in the treated group (85.4%) compared to the control group (47.2%). Conversely, the treatment had a significant impact on the fertilization rate, with a lower rate observed in the treated group (66.9%) compared to the control group (75.0%) [ 91 ].…”

Section: Sperm Sexing Through Immunological Methods: a Promising Weaponmentioning

confidence: 99%

“…Moderate to high cost depending on the specific method and reagents used [15,76,90] May be challenging to scale up [88] Moderate to high impact on production efficiency. It requires additional processing steps and may have limitations in terms of speed and accuracy depending on the target [15,88,91] Sperm sexing through CRISPR-Cas9 genetic tools Relatively high cost due to the need for gene editing reagents and equipment [96,97] Can be challenging to scale up [95,96] High potential for production efficiency if optimized properly. It offers precise genetic manipulation, but further development and validation are needed for commercial applications [94][95][96] Sperm sexing with magnetic nanoparticles Moderate to high cost due to the need for specialized nanoparticles and equipment [100] Can be scaled up, but may require optimization for large-scale implementation [113,114] Moderate to high impact on production efficiency.…”

Section: Sexing Principle Cost Scalability Production Efficiencymentioning

confidence: 99%

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The History and Prospects of Rabbit Sperm Sexing

Pinto-Pinho,

Ferreira,

Pinto-Leite

et al. 2023

Veterinary Sciences

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Sperm sex selection is a longstanding challenge in the field of animal reproduction. The cuniculture industry, in particular producers of males or females for breeding purposes, would greatly benefit from the pre-selection of the offspring’s sex. This review article overviews the current and future developments in rabbit sperm sexing technologies, as well as the implications of implementing these methodologies in cuniculture. The first attempts of sperm sexing were performed in rabbits; however, a both efficient and cost-effective methodology was not yet developed for this species. Those included sperm sexing according to differences in sperm density, surface electric charge, pH susceptibility, antisera reaction, and flow cytometry. Separation by flow cytometry has proven to be efficient in rabbits, yielding fractions with approximately 81% and 86% purity for X- and Y-sperm, respectively. However, it is not cost-effective for cuniculture and decreases sperm quality. The advantages, limitations, and practical considerations of each method are presented, highlighting their applicability and efficiency. Furthermore, herein we explore the potential of immunological-based techniques that overcome some of the limitations of earlier methods, as well as recent advancements in sperm sexing technologies in other animal models, which could be applied to rabbits. Finally, the challenges associated with the development and widespread implementation of rabbit sperm sexing technologies are addressed. By understanding the advantages and limitations of existing and emerging methods, researchers can direct their efforts towards the most promising directions, ultimately contributing to a more efficient, profitable, and sustainable cuniculture.

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“…The company describes simple procedures, such as mixing the semen sample with the commercial vial and carrying out AI, in the case of the WholeMan kit, or mixing, incubating for 20-30 min, and then conducting AI, if using the WholeMom kit intended for heifers. Some variations are employed, such as an overnight incubation when using the WholeMom kit for sows (12,64).…”

Section: Animal Production Systems and The Role Of Reproductive Techn...mentioning

confidence: 99%

Sustainable animal production: exploring the benefits of sperm sexing technologies in addressing critical industry challenges

Quelhas,

Pinto-Pinho,

Lopes

et al. 2023

Front. Vet. Sci.

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The sex of the animals is of paramount importance in many animal production systems. This is particularly evident in the production of milk or in breeding programs focused on the production of female animals. In some cases, slaughter or euthanasia of animals of the unwanted sex becomes the only solution, highlighting ethical and economic concerns. As global demand for food continues to rise, the importance of addressing these issues becomes more evident. Reproductive technologies, such as sperm sexing techniques, may hold the key to addressing both animal welfare and the sustainability of animal production. The use of semen enriched with sperm capable of producing offspring of the desired sex can serve as a valuable tool for producers to exert greater control over production outcomes, not only helping to mitigate welfare issues related to the unnecessary premature death of unwanted offspring but also providing a possible ally in the face of stricter animal welfare guidelines. In addition, sexed semen can also contribute to financial gains and reduce greenhouse gas emissions and food waste associated with the less profitable part of the herd. This paper explores the positive impacts that sperm sexing can have on animal welfare, economy, and environment. It also discusses currently available options and strategies for more successful implementation of sexed semen. Partnerships between companies and scientists will be essential to find innovative ways to adapt current production systems and develop sperm sexing technologies that apply to most livestock industries.

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Analysis of sex ratio on bovine in vitro fertilized embryos using sex determination kit treated sperm

Cited by 4 publications

References 17 publications

Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics

Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics

The History and Prospects of Rabbit Sperm Sexing

Sustainable animal production: exploring the benefits of sperm sexing technologies in addressing critical industry challenges

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