Analysis of Shrimp Hemolymph and Ionic Modification of a<i>Penaeus</i>Cell Culture Formulation

Najafabadi, Ali K.; Ellender, R. D.; Middlebrooks, B. L.

doi:10.1577/1548-8667(1992)004<0143:aoshai>2.3.co;2

Cited by 26 publications

(16 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, a medium exclusively for in vitro growth of shrimp cell cultures has not been designed, and the fact that an appropriate medium is required to establish shrimp cell lines in tune with the quantum change which the Grace's insect cell culture medium [29][30][31][32] has brought about; ever since the publication of Grace's insect cell culture medium, over 500 insect cell lines could be established [53,76]. Likewise, to formulate an exclusive shrimp cell culture medium, indepth analysis of the biochemistry of body fluids [64,74] is the prime requirement. Moreover, to tide over the difficulties in developing a complete medium for shrimp cell culture, attention must be directed towards satisfying the nutritional requirements of each cell type.…”

Section: Cell Culture Medium: a Stepping Stone For Cell Line Developmentmentioning

confidence: 99%

“…Moreover, six authors (16 %) selected Penaeus stylirostris [49,51,63,73,74,78] as the donor animal of tissues and organs. Besides, in two publications (4 %) Penaeus indicus [46,82] and Penaeus aztecus [18,64] were the species used. There is only one report (2 %) of using P. penicillatus [10] for extracting tissues and organs for cell culture development (Fig.…”

Section: Species Of Choice: a Major Concernmentioning

confidence: 99%

“…[27,46,62,80]. Besides, testis [62,81], heart [9,10,48,62,80], hepatopancreas [9,28,48,55,58,62,64,81,89], gill [9,62], nerve [9,13,48,62,80,81], muscle [9,27,48], hematopoietic tissue [11,62,90], embryonic tissue [19,80,82], epidermis [81,82], gut [9,62] and Y organ [81] were also widely used for cell culture development (Fig. 3).…”

Section: Tissues and Organs Used For Cell Culture Developmentmentioning

confidence: 99%

See 2 more Smart Citations

Establishment of Shrimp Cell Lines: Perception and Orientation

2012

View full text Add to dashboard Cite

Development of continuous shrimp cell lines for effective investigation on shrimp viruses remains elusive with an arduous history of over 25 years. Despite presenting challenges to researchers in developing a cell line, the billion dollar aquaculture industry is under viral threat. Advances in molecular biology and various gene transfer technologies for immortalization of cells have resulted in the development of hundreds of cell lines from insects and mammals, but yet not a single cell line has been developed from shrimp and other marine invertebrates. Though improved growth and longevity of shrimp cells in vitro could be achieved by using modified growth media this did not make any leap to spontaneous transformation; probably due to the fact that shrimp cells inhibited neoplastic transformations. Oncogenic induction and immortalization are considered as the possible ways, and an exclusive medium for shrimp cell culture and an appropriate mode of transformation are crucial. In this review status of shrimp cell line development and its future orientation are discussed.

show abstract

Section: Cell Culture Medium: a Stepping Stone For Cell Line Developmentmentioning

confidence: 99%

Section: Species Of Choice: a Major Concernmentioning

confidence: 99%

Section: Tissues and Organs Used For Cell Culture Developmentmentioning

confidence: 99%

See 1 more Smart Citation

Establishment of Shrimp Cell Lines: Perception and Orientation

2012

View full text Add to dashboard Cite

show abstract

“…As the analysis of major ions in the hemolymph is crucial to formulate cell culture medium (Najafabadi et al 1992), metal ion strength of haemolymph and seawater at 27 % salinity were analyzed using Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES Thermo Electron IRIS INTREPID II XSP DUO). Before analysis, seawater was sterilized by autoclaving at 15 lbs for 15 min, filtered through Whatman No.…”

Section: Analysis Of Metal Ionsmentioning

confidence: 99%

“…Mulford et al (2001) revealed that 2 9 Leibovitz's medium supplemented with 10 % (v/v) heat inactivated FBS, 5 % (v/v) muscle extract, 0.06 g l -1 of L-proline, 1 g l -1 glucose prepared in 25 % seawater was effective for cell migration, survival and longevity. Though the hemolymph composition of P. aztecus and P. stylirostris had been reported earlier (Najafabadi et al 1992;Shimizu et al 2001), a medium exclusively for shrimp cell culture based on it could not be attained so far, other than the modification of the existing media. This might be cited as one of the reasons for the non attainment of immortal cell line from shrimp.…”

Section: Introductionmentioning

confidence: 99%

A novel medium for the development of in vitro cell culture system from Penaeus monodon

et al. 2012

View full text Add to dashboard Cite

Lack of a valid shrimp cell line has been hampering the progress of research on shrimp viruses. One of the reasons identified was the absence of an appropriate medium which would satisfy the requirements of the cells in vitro. We report the first attempt to formulate an exclusive shrimp cell culture medium (SCCM) based on the haemolymph components of Penaeus monodon prepared in isosmotic seawater having 27 % salinity. The SCCM is composed of 22 amino acids, 4 sugars, 6 vitamins, cholesterol, FBS, phenol red, three antibiotics, potassium dihydrogen phosphate and di-sodium hydrogen phosphate at pH 6.8-7.2. Osmolality was adjusted to 720 ± 10 mOsm kg -1 and temperature of incubation was 25 8C. The most appropriate composition was finally selected based on the extent of attachment of cells and their proliferation by visual observation. Metabolic activity of cultured cells was measured by MTT assay and compared with that in L-15 (29), modified L-15 and Grace's insect medium, and found better performance in SCCM especially for lymphoid cells with 107 % increase in activity and 85 ± 9 days of longevity. The cells from ovary and lymphoid organs were passaged twice using the newly designed shrimp cell dissociation ''cocktail''.

show abstract

The Development and Characterization of a Cell Culture System from Indian Mud Crabs Scylla serrata

et al. 2019

View full text Add to dashboard Cite

Commercially available culture media and supplements were tested for their potential to produce primary cell cultures from tissues of Indian mud crabs Scylla serrata. Eight commercially available culture media from Sigma-Aldrich (Leibovitz's L-15, Medium 199, Grace's Insect Medium, Minimal Essential Medium, Dulbecco's Modified Eagle Medium, TC-100 Insect Medium, IPL-41 Insect Medium, and Roswell Park Memorial Institute) were examined. Three different supplements (amino acid and sugar [AS], crab muscle extract [CME], and natural seawater [NSW]) were also examined. The hemocyte culture appeared to grow well for a maximum period of 21 d in 2 × L-15 medium supplemented with AS and 15% fetal bovine serum (FBS). Partial amplification and sequencing of the cytochrome oxidase subunit I (COI) gene confirmed that the primary hemocytes originated from Indian mud crabs. The effects of four metals on hemocyte viability were evaluated using the MTT assay. Of the four metals examined (arsenic, lead, cobalt, and nickel), cobalt and nickel were more toxic to the crab cells than the other metals. Both acridine orange/ethidium bromide and Hoechst staining showed the presence of apoptosis and necrosis in metal-treated groups, which suggests that metals in an aquatic environment induce death of the Indian mud crab's hemocytes. The hemocyte primary cell culture was also used to study the cytotoxicity effect of bacterial extracellular products from Vibrio harveyi and white spot syndrome virus. This study demonstrates that hemocyte primary cell culture can be used as a tool to study viral and bacterial pathogenesis and to assess the cytotoxicity of pollutants present in aquatic environments.

show abstract

Analysis of Shrimp Hemolymph and Ionic Modification of aPenaeusCell Culture Formulation

Cited by 26 publications

References 4 publications

Establishment of Shrimp Cell Lines: Perception and Orientation

Establishment of Shrimp Cell Lines: Perception and Orientation

A novel medium for the development of in vitro cell culture system from Penaeus monodon

The Development and Characterization of a Cell Culture System from Indian Mud Crabs Scylla serrata

Contact Info

Product

Resources

About