Analysis of simple sequence repeat markers in homozygous lines of apple

Höfer, Monika; Gomez, Arturo Ardila; Aguiriano, E.; Manzanera, J. A.; Bueno, M. A.

doi:10.1046/j.1439-0523.2002.00676.x

Cited by 30 publications

(19 citation statements)

References 11 publications

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“…This somaclonal variation has been detected with RAPDs (Raimondi et al 2001), producing a changes similar to the obtained by Eimert et al (2003). The EST-SSRs have been used to determine the origin of the regenerated plantlets from anther culture in different species such as rice (Lapitan et al 2009), apple (Höfer et al 2002;Vanwynsberghe et al 2005;Okada et al 2009), corn (Aulinger et al 2003), soy (Rodrigues et al 2004), potato (Veilleux et al 1995;Chani et al 2000), coconut (Perera et al 2008), orange (Cao et al 2011) or eggplant (Salas et al 2011).…”

Section: Discussionmentioning

confidence: 89%

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Regalado

Martín

Madrid

et al. 2015

Plant Cell Tiss Organ Cult

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Anther culture is used to develop asparagus ''super-male'' (di-haploids) in asparagus, which can be used to develop ''all-male'' varieties, by crossing them with suitable females; their progenies will be formed only by males which is advantageous for producers. This report describe a new anther culture protocol adapted to ''Morado de Huétor'', a Spanish tetraploid landrace, and studied the different factors involved in callus proliferation success from anther explants such as the microspore development stage, or the type of stress used to induce the symmetric division of the microspores, to obtain a high success rate (90 %). For plantlets regenerates from anther culture (PRACs) regeneration we develop a proliferation media supplemented with a combination of pCPA and BA able to induce callus proliferation and plantlet regeneration in the same step in a 50 % of calli, simplifying the procedure. The high percentage of heterozygous male recovery, originated from somatic cells, is an important problem in the anther culture, and to elucidate the origin of PRACs we have combined different tools: ploidy analysis, characterization with the linked sex-marker Asp1-T7 and with ESTSRRs. We can establish that 50 % of PRACs obtained in this work were regenerated from diploid microspores of ''Morado de Huétor'', regenerating diploid, di-diploid and tetra-diploid plantlets. The di-diploids males (MMmm) would generate a ratio male:female of 5:1 (83.3 %) and the tetra-diploid males (MMMMmmmm) a ratio male:female of 69:1 (98.6 %), so the tetra-diploid males could be considered ''super-males'' and be used to develop ''all-male'' varieties of ''Morado de Huétor''.

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Section: Discussionmentioning

confidence: 89%

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Regalado

Martín

Madrid

et al. 2015

Plant Cell Tiss Organ Cult

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“…Mendelian inheritance and high polymorphism make nuclear SSR markers very efficient for the evaluation of heterozygosity (vs homozygosity) in diploids (Chani et al, 2000;Hofer et al, 2002;Fei Yu et al, 2004;Vanwynsberghe et al, 2005). However, null alleles are frequently observed and have to be considered for genetic analysis (Dakin and Avise, 2004;Hoffman and Amos, 2005).…”

Section: Discussionmentioning

confidence: 99%

Molecular evidence for the natural production of homozygous Cupressus sempervirens L. lines by Cupressus dupreziana seed trees

et al. 2009

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“…Triploids regenerated from anther culture have been reported in apple (Ho¨fer, 1994;Ho¨fer et al, 2002). Isozyme analyses have been employed to confirm the gametic origin of calluses and plantlets in apple (Ho¨fer and Grafe, 2000).…”

Section: Characterization and Propagation Of Regenerants Obtained By mentioning

confidence: 99%

Doubled haploid production in fruit crops

Germanà

2006

Plant Cell Tiss Organ Cult

130

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The interest of fruit breeders in haploids and doubled haploids (DH), lies in the possibility of shortening the time needed to produce homozygous lines compared to conventional breeding. Haplo-diploidization through gametic embryogenesis allows single-step development of complete homozygous lines from heterozygous parents. In a conventional breeding programme, a pure line is developed after several generations of selfing. With fruit crops, characterized by a long reproductive cycle, a high degree of heterozygosity, large size, and, sometimes, self-incompatibility, there is no way to obtain haploidization through conventional methods. This paper reviews the current status of research on doubled haploid production in the main fruit crops:

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Analysis of simple sequence repeat markers in homozygous lines of apple

Cited by 30 publications

References 11 publications

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Molecular evidence for the natural production of homozygous Cupressus sempervirens L. lines by Cupressus dupreziana seed trees

Doubled haploid production in fruit crops

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