Analysis of soil and dust samples for polychlorinated biphenyls by enzyme-linked immunosorbent assay (ELISA)

Chuang, Jane C.; Miller, Larry S.; Davis, Dave B; Peven, Carole S.; Johnson, J. C.; Emon, Jeanette M. Van

doi:10.1016/s0003-2670(98)00439-5

Cited by 27 publications

(10 citation statements)

References 2 publications

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“…The results of cross-reactivity studies showed that the assay was highly specific. Chuang et al [50] also used pooled rabbit anti-PCB antiserum (AC-3) to determine PCBs in soil, and obtained a correlation coefficient between GC/ECD and ELISA of 0.91 for 41 soil samples. In addition, a sheep polyclonal antibody was developed, which had no cross-reaction with 19 other structurally related compounds, and only 5% cross-reactivity with dichlorodiphenyledichloroethylene (DDE) [91].…”

Section: Detection Methodsmentioning

confidence: 99%

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Tian

Xie

et al. 2012

Sensors

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With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.

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Section: Detection Methodsmentioning

confidence: 99%

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Tian

Xie

et al. 2012

Sensors

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“…The sampler can be used to collect cores without liners for immediate examination or with liners to obtain an undisturbed and sealed sample. The collected soil samples were analyzed using enzyme-linked immunosorbent assay (ELISA) method (Chuang et al 1998(Chuang et al , 2003Gilvanda et al 1998;Amin et al 1998;Watanabe et al 2004;and Musick et al 2000).…”

Section: Soil Sampling and Analysismentioning

confidence: 99%

Pesticide Impact Assessment via Using Enzyme-linked Immunosorbent Assay (ELISA) Technique in the Lower Rio Grande River Basin, Texas

et al. 2009

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This paper presents a field survey using health impact assessment approach trying to: (1) demonstrate a basic soil and groundwater survey/sampling, (2) investigate any possible long-term pesticide contamination in connection with citrus orchard pesticide application, and (3) conduct an assessment review of possible pesticide impacts on public health in the Lower Rio Grande Valley (LRGV), Texas. A sampling plan was prepared to collect samples that include soil from a citrus orchard of Texas A&M Agricultural Research and Extension Center, Weslaco, located in the Hidalgo county and groundwater from domestic and public wells located in the neighboring LRGV region. Field investigation of the contamination was performed using enzymelinked immunosorbent assay (ELISA) technique and the analytical results indicated that there is no considerable concentration of simazine pesticide in soil and groundwater. All of the measurements are below the maximum contamination limit (MCL) value provided by US EPA. A statistical assessment was also performed on the cancer mortality rates in the LRGV region to identify any significant health concerns in this region. After reviewing several relevant reports, it is inferred that there are no apparent health issues in the LRGV region in relation to such pesticide impact and

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“…This might be a partial cause of the discrepancies between ELISA and GC results. The sample extraction procedure plays a major role in the overall analytical result even the accuracy and precision of ELISA for analysis of spiked samples are high [2,8,13]. The data obtained by Johnson and Van Emon [8] demonstrated that ELISA was capable of providing quantitative results for PCB when Soxhlet or supercritical-fluid extraction was used.…”

Section: Analysis Of Real Soil Samplesmentioning

confidence: 99%

“…Several PCB test kits are commercially available for semi-quantitative analysis of PCB in different matrixes, and considerable method-development activity has been focused on the quantitative determination of PCB in soil [1,2,8,12,13,14].…”

Section: Introductionmentioning

confidence: 99%

Direct competitive ELISA for the determination of polychlorinated biphenyls in soil samples

et al. 2002

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A direct competitive ELISA for determination of polychlorinated biphenyls (PCB) in soil samples was described. The standard calibration curve based on Delor 103 (Aroclor 1242) was constructed in the dynamic range of 10-1000 micro g L(-1) and a detection limit of 5.0-12.9 micro g L(-1) (or 0.5-1.29 micro g g(-1) soil) was achieved. When spiked soil samples were extracted with methanol recoveries were 90.6-106.3 %. The effect of methanol and DMSO on assay signal and sensitivity was established. Eight PCB-contaminated soil samples were analyzed by ELISA and gas chromatography (GC). The ELISA results from Soxhlet extraction were in a good agreement with those of GC (correlation coefficient 0.9866; n=8). Except for one soil sample the results from ELISA with methanol extraction were not significantly different from those from GC.

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Analysis of soil and dust samples for polychlorinated biphenyls by enzyme-linked immunosorbent assay (ELISA)

Cited by 27 publications

References 2 publications

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Pesticide Impact Assessment via Using Enzyme-linked Immunosorbent Assay (ELISA) Technique in the Lower Rio Grande River Basin, Texas

Direct competitive ELISA for the determination of polychlorinated biphenyls in soil samples

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