Analysis of some fibrate-type antihyperlipidemic drugs by AMD

Planar Chromatography

“…Automated multiple development (AMD) is carried out in a Camag chamber with increasing development lengths and an isocratic mobile phase or a mobile phase gradient with decreasing strength. Reported applications include the stability indicating HPTLC assay of leuprolide acetate [silica gel 60 layer, five step incremental multiple development with ethyl acetate−MeOH−25% aqueous ammonia (6:3:1), densitometry at 280 nm] ; analysis of some fibrate-type antihyperlipidemic drugs (diol F 254 plates, THF−hexane mobile phase, densitometry at 245 and 277 nm) ; analysis of ginsenosides from Panax quinquefolium L (silica gel 60F 254 , MeOH−chloroform gradients, detection with Godin’s reagent, densitometry at 540 nm) ; and separation and quantification of terpenoids of Boswellia serrata Roxb. (HPTLC silica gel F layer; mobile phase gradients; densitometry at 254 nm or 560 nm after derivatization with anisaldehyde−sulfuric acid reagent) .…”