Analysis of spatial and temporal variations in bacterial community dynamics within stream biofilms

Gautam, Anju; Lear, Gavin; Lewis, Gillian

doi:10.1080/00288330.2020.1804409

Cited by 3 publications

(10 citation statements)

References 71 publications

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“…PERMANOVA models used previously (Gautam et al ., 2020), but incorporating substantial additional data, confirmed that the bacterial community composition varied between streams and over time, supporting observations from previous studies (Lear et al ., 2008; Hassell et al ., 2018). Proteobacteria formed the largest relative fraction of the bacterial community in our dataset; they are recognized as the most common dominant phylum in benthic and hyporheic biofilms overall (Battin et al ., 2016).…”

Section: Discussionsupporting

confidence: 87%

“…PCR amplification and library preparation were performed as described in Gautam et al . (2020). Sequencing was conducted by Auckland Genomics (University of Auckland, New Zealand) on an Illumina MiSeq instrument using 2 × 300 bp chemistry.…”

Section: Methodsmentioning

confidence: 99%

“…Auckland Council collected physicochemical stream water attributes, adhering to clear data collection standards (APHA, 1998) and collated them in a National Institute of Water and Atmospheric Research (NIWA; https://cliflo.niwa.co.nz/) database. At each location and sample date, the data for this study were collated as described by Gautam et al (2020).…”

Section: Sample Collection Processing and Dna Extractionmentioning

confidence: 99%

“…Advances in molecular methods, including high‐throughput sequencing techniques for determining bacterial community composition and diversity, based on 16S rRNA gene sequences (Good et al ., 2018), provide collective evidence that bacteria exhibit substantial spatial and temporal patterns in their distribution and abundance (Lear et al ., 2008; Esposito et al ., 2016; Gautam et al ., 2020). Highly dominant and diverse bacterial phyla reported among freshwater environments include Proteobacteria, Cyanobacteria, Bacteroidetes, Verrucomicrobia, Actinobacteria and Planctomycetes (Besemer et al ., 2012; Battin et al ., 2016), but their dominance varies greatly over time and geographic location.…”

Section: Introductionmentioning

confidence: 99%

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Time after time: detecting annual patterns in stream bacterial biofilm communities

Gautam

Lear

Lewis

2022

Environmental Microbiology

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Summary To quantify the major environmental drivers of stream bacterial population dynamics, we modelled temporal differences in stream bacterial communities to quantify community shifts, including those relating to cyclical seasonal variation and more sporadic bloom events. We applied Illumina MiSeq 16S rRNA bacterial gene sequencing of 892 stream biofilm samples, collected monthly for 36‐months from six streams. The streams were located a maximum of 118 km apart and drained three different catchment types (forest, urban and rural land uses). We identified repeatable seasonal patterns among bacterial taxa, allowing their separation into three ecological groupings, those following linear, bloom/trough and repeated, seasonal trends. Various physicochemical parameters (light, water and air temperature, pH, dissolved oxygen, nutrients) were linked to temporal community changes. Our models indicate that bloom events and seasonal episodes modify biofilm bacterial populations, suggesting that distinct microbial taxa thrive during these events including non‐cyanobacterial community members. These models could aid in determining how temporal environmental changes affect community assembly and guide the selection of appropriate statistical models to capture future community responses to environmental change.

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Section: Discussionsupporting

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Sample Collection Processing and Dna Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Time after time: detecting annual patterns in stream bacterial biofilm communities

Gautam

Lear

Lewis

2022

Environmental Microbiology

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“…Although stream biofilms are assumed to assemble from the suspended bacterial community present in the water column, biofilm microbial assemblages are noticeably different from free-flowing communities [19]. As processes such as species sorting reduce stream biofilm diversity through competition, the biofilm diversity is believed to be maintained by a continuous inflow of microorganisms from upstream catchments [19,20]. Vice versa, stream biofilms may also promote the dispersal of aquatic bacterial species within aquatic networks, for example when biofilms release microbes and thereby reinoculate the free-flowing stream community with these taxa [17].…”

Section: Introductionmentioning

confidence: 99%

Soil microbial inoculation during flood events shapes headwater stream microbial communities and diversity

et al. 2021

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Flood events are now recognized as potentially important occasions for the transfer of soil microbes to stream ecosystems. Yet, little is known about these “dynamic pulses of microbial life” for stream bacterial community composition (BCC) and diversity. In this study, we explored the potential alteration of stream BCC by soil inoculation during high flow events in six pre-alpine first order streams and the larger Oberer Seebach. During 1 year, we compared variations of BCC in soil water, stream water and in benthic biofilms at different flow conditions (low to intermediate flows versus high flow). Bacterial diversity was lowest in biofilms, followed by soils and highest in headwater streams and the Oberer Seebach. In headwater streams, bacterial diversity was significantly higher during high flow, as compared to low flow (Shannon diversity: 7.6 versus 7.9 at low versus high flow, respectively, p < 0.001). Approximately 70% of the bacterial operational taxonomic units (OTUs) from streams and stream biofilms were the same as in soil water, while in the latter one third of the OTUs were specific to high flow conditions. These soil high-flow OTUs were also found in streams and biofilms at other times of the year. These results demonstrate the relevance of floods in generating short and reoccurring inoculation events for flowing waters. Moreover, they show that soil microbial inoculation during high flow enhances microbial diversity and shapes fluvial BCC even during low flow. Hence, soil microbial inoculation during floods could act as a previously overlooked driver of microbial diversity in headwater streams.

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Pelagic Bacteria, Archaea, and Viruses

McMahon,

Newton

2024

Wetzel's Limnology

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Analysis of spatial and temporal variations in bacterial community dynamics within stream biofilms

Cited by 3 publications

References 71 publications

Time after time: detecting annual patterns in stream bacterial biofilm communities

Time after time: detecting annual patterns in stream bacterial biofilm communities

Soil microbial inoculation during flood events shapes headwater stream microbial communities and diversity

Pelagic Bacteria, Archaea, and Viruses

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