As an exquisite asset of Chinese traditional culture, traditional opera occupies a place of high esteem within the world’s cultural and artistic treasury. The impact of emerging cultures has threatened the future of traditional opera culture, necessitating a thorough examination of the historical context of the Grand Canal and traditional opera. There is insufficient research on the spatial evolution of the traditional opera culture along the Grand Canal; thus, this study takes ancient opera stages, a representative cultural relic of traditional opera, as an entry point and employs methods such as kernel density analysis and standard deviation ellipse analysis to analyze the spatial and temporal distribution patterns of the traditional opera culture along the Grand Canal. The results showed that: (i) Nationwide, opera stages in the areas along the Grand Canal exhibit a significant clustering characteristic. (ii) The changes in the number and locations of opera stages in the areas along the Grand Canal are closely related to the rise and fall of the Canal. The opera stages emerged along the Canal, gradually prospered with the development of the Canal, and finally clustered in a band-like cluster along the Grand Canal. (iii) From the Ming Dynasty to the founding of the People’s Republic of China, the opera stages in the areas along the Grand Canal spread in the “southeast–northwest” direction, which was consistent with the main direction of the Grand Canal, indicating its driving influence. (iv) On the centennial scale, from the 14th century to the 20th century, the evolution characteristics of the distribution centroid of opera stages in the areas along the Grand Canal were closely related to the key time nodes of Grand Canal construction and basin expansion. This study reveals the relationship between the Grand Canal and the spatial pattern evolution of traditional opera culture, aiming to promote the construction of the Grand Canal cultural belt.