Analysis of spatio-temporal fungal growth dynamics under different environmental conditions

Ligne, Liselotte De; Ulzurrun, Guillermo Vidal-Diez de; Baetens, Jan; Bulcke, Jan Van den; Acker, Joris Van

doi:10.1186/s43008-019-0009-3

Cited by 36 publications

(36 citation statements)

References 68 publications

(108 reference statements)

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“…4). Therefore, this study demonstrates that the growth factors also monitor the quality and variation in the fungal species as described by previous studies (Kazemian, 2019, Ligne, 2019. The reason behind these enormous variation in fungal species is clear i.e., various combination of temperatures, oxygen contents and moisture levels are optimum for the germination of different fungal spores and probably also responsible for the formation of fruit bodies.…”

Section: Discussionsupporting

confidence: 79%

Impact of Field and Laboratory Environmental Conditions on the Diversity of Wood-Decay Fungi in the Forests of Northwest Arkansas

Alshammari¹,

Veettil²,

Sulieman³

et al. 2020

J Pure Appl Microbiol

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Environmental factors such as temperature and humidity directly affect the growth and fruit bodies of fungi. We studied the diversity of wood decaying fungal species, which have grown on same substrate in forest as well as laboratory environment. Ten specimens of fruit body of wood-decaying fungi and 24 random pieces of coarse wooden debris were collected from the forest of northwest Arkansas. The samples of coarse woody debris were incubated in laboratory-growth chambers for two months to promote the fungal growth. Fourty-two different species of wood-decay fungal isolates were recovered and identified by internal transcribed spacer (ITS) region sequencing. The isolates from the forest belonged to twenty-two different taxa whereas twenty taxonomic groups were reported from the growth compartments. Remarkably, data observed from two sets did not shared any taxon. These results indicated that environmental growth conditions play crucial role on fungal diversity even if grown on same substrates.

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Section: Discussionsupporting

confidence: 79%

Impact of Field and Laboratory Environmental Conditions on the Diversity of Wood-Decay Fungi in the Forests of Northwest Arkansas

Alshammari¹,

Veettil²,

Sulieman³

et al. 2020

J Pure Appl Microbiol

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“…From an equipment perspective we believe that slightly-more sophisticated measurement tools may be useful. For example, simple flat-bed document scanners could be used to achieve time-lapse imaging of radial extension to enable more precise quantification of extension dynamics [97]. Additionally, flat-bed scanners have been used to quantify the total number of hyphal tips, total number of hyphal segments, total number of network nodes, total length of the mycelium, growth angle, and branching angle [97].…”

Section: Discussionmentioning

confidence: 99%

“…For example, simple flat-bed document scanners could be used to achieve time-lapse imaging of radial extension to enable more precise quantification of extension dynamics [97]. Additionally, flat-bed scanners have been used to quantify the total number of hyphal tips, total number of hyphal segments, total number of network nodes, total length of the mycelium, growth angle, and branching angle [97]. Furthermore, using time-lapse imaging could speed up determination of extension rates and reduce the size of the plates being used; transitioning from 9 cm plates to 24-well culture plates could enable increased throughput and sample numbers, and better support statistical analysis of differences in strain performance.…”

Section: Discussionmentioning

confidence: 99%

Enabling community-based metrology for wood-degrading fungi

Perez

Luccioni

Kamakaka

et al. 2020

Fungal Biol Biotechnol

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Background: Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on single-cell organisms and extensive ancillary equipment to produce precursors for downstream manufacturing processes. Alternative forms of bioproduction based on solid-state fermentation and wood-degrading fungi could enable more direct means of manufacture. However, basic methods for cultivating wood-degrading fungi are often ad hoc and not readily reproducible. Here, we developed standard reference strains, substrates, measurements, and methods sufficient to begin to enable reliable reuse of mycological materials and products in simple laboratory settings. Results:We show that a widely-available and globally-regularized consumer product (Pringles ™ ) can support the growth of wood-degrading fungi, and that growth on Pringles ™ -broth can be correlated with growth on media made from a fully-traceable and compositionally characterized substrate (National Institute of Standards and Technology Reference Material 8492 Eastern Cottonwood Whole Biomass Feedstock). We also establish a Relative Extension Unit (REU) framework that is designed to reduce variation in quantification of radial growth measurements. So enabled, we demonstrate that five laboratories were able to compare measurements of wood-fungus performance via a simple radial extension growth rate assay, and that our REU-based approach reduced variation in reported measurements by up to ~ 75%. Conclusions:Reliable reuse of materials, measures, and methods is necessary to enable distributed bioproduction processes that can be adopted at all scales, from local to industrial. Our community-based measurement methods incentivize practitioners to coordinate the reuse of standard materials, methods, strains, and to share information supporting work with wood-degrading fungi.

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“…Although we were not able to conduct compositional analysis of the flavored From an equipment perspective we believe that slightly-more sophisticated measurement tools may be useful. For example, simple flat-bed document scanners could be used to achieve time-lapse imaging of radial extension to enable more precise quantification of extension dynamics 88 .…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, flat-bed scanners have been used to quantify the total number of hyphal tips, total number of hyphal segments, total number of network nodes, total length of the mycelium, growth angle, and branching angle 88 . Furthermore, using time-lapse imaging could speed up determination of extension rates and reduce the size of the plates being used; transitioning from 9 cm plates to 24-well culture plates could enable increased throughput and sample numbers, and better support statistical analysis of differences in strain performance.…”

Section: Discussionmentioning

confidence: 99%

Enabling community-based metrology for wood-degrading fungi

Perez

Luccioni

Gaut

et al. 2019

Preprint

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Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on single-cell organisms and extensive ancillary equipment to produce precursors for downstream manufacturing processes. Alternative forms of bioproduction based on solid-state fermentation and wood-degrading fungi can enable more direct means of manufacture. However, such practices are often ad hoc and not readily reproducible. We sought to develop standard reference strains, substrates, measurements, and methods sufficient to begin to enable reliable reuse of mycological materials and products. Specifically, we show that a widely-available and globallyregularized consumer product (Pringles™) can support the growth of wood-degrading fungi, and that growth on Pringles™ can be correlated with growth on a fully-traceable and compositionally characterized substrate (NIST Reference Material 8492 Eastern Cottonwood Biomass). So established, five laboratories were able to compare measurements of wood-fungus performance via a simple radial extension growth rate assay. Reliable reuse of materials, measures, and methods is necessary to enable distributed bioproduction processes that can be adopted at all scales, from local to industrial. cells, known as hyphae, chitinous-composite cell walls, vast repertoire of degradative enzymes and secondary metabolites, and interconnected networks of cells, known as mycelium, that form through a combination of apical growth, hyphal branching, and regulated self-fusion 22-31 . Growing mycelium penetrate and degrade the substrate upon which the organism lives and uptake and shuttle nutrients throughout complex hyphal networks 23 .Mushrooms, the reproductive structures of wood-degrading filamentous fungi, are widely-used as foodstuffs and as a source of medicines and are consequentially revered by many cultures [30][31][32] .Additionally, mycological materials made from the tissues of mushrooms are used in meat replacements, filtration and remediation of contaminated water, packaging materials, furniture, textiles for the fashion industry, architectural design, art, materials for super capacitors and batteries, anti-viral therapeutics for bees, and nanoparticle synthesis 35,36,45,46,[37][38][39][40][41][42][43][44] . Most commercial mycological materials are fabricated by placing an organism and substrate mixture into a preformed mold and allowing the mycelium to process the substrate via solid-state fermentation. The process itself is typically halted via thermal inactivation followed by downstream processing.Recent studies have elucidated the impacts that choice of organism, genetic modification, substrate composition, and environmental conditions have on the macroscopic properties of mycelium-based materials [47][48][49][50][51][52][53][54] . Most organisms now used to produce mycelium materials are of the class Agaricomycete in the division Basidiomycota. The Agaricomycete are less-extensively studied and developed as some of their Ascomycota counterparts, such as ...

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Analysis of spatio-temporal fungal growth dynamics under different environmental conditions

Cited by 36 publications

References 68 publications

Impact of Field and Laboratory Environmental Conditions on the Diversity of Wood-Decay Fungi in the Forests of Northwest Arkansas

Impact of Field and Laboratory Environmental Conditions on the Diversity of Wood-Decay Fungi in the Forests of Northwest Arkansas

Enabling community-based metrology for wood-degrading fungi

Enabling community-based metrology for wood-degrading fungi

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