“…The surface of a porcine heart collected from a local abattoir was prepared for DIC analysis applying a speckle pattern using the approach described in a previous work [16]. Briefly, the cardiac surface was firstly coated with a blue dye (Methylene blue, Sigma-Aldrich Company Ltd, Dorset, UK) to create a dark background, then white speckles were applied with an airbrush (124 kPA working pressure, 0.3 mm nozzle diameter, approximately 15 cm spraying distance, Iwata Hi-Line HP-CH, Anest Iwata-Medea Inc., Portland, OR, USA.)…”