Analysis of steroids Part 50. Derivatization of ketosteroids for their separation and determination by capillary electrophoresis

Gǒrög, Sándor; Gazdag, M.; Kemenes-Bakos, P

doi:10.1016/0731-7085(95)01693-7

Cited by 18 publications

(4 citation statements)

References 11 publications

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“…Numerous steroidal isomers and closely related molecules within the biomatrix can co-elute and share m/z values and thus the field must continue to explore orthogonal separation to peel away the layers of chemical complexity. Capillary electrophoresis drew early interest as an alternative separation approach [33,34] but this has not yet reached common use. In contrast however, ultra-performance supercritical fluid chromatography (UP-SFC) has found a renaissance with revised instrument design enabling safety and throughput [35].…”

Section: What Lies Beneathmentioning

confidence: 99%

Mass spectrometry: Future opportunities for profiling and imaging steroids and steroid metabolites

Andrew

Homer

2020

Current Opinion in Endocrine and Metabolic Research

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Steroid hormone profiling has historically underpinned advances in endocrine investigation and research, crucially dependent on selective and sensitive hormone assays. Mining the "steroidome" by mass spectrometry (MS) provides greater specificity than immunoassays. Building on a 50 year legacy, gas and liquid chromatography-MS continue to evolve (e.g. sequential derivatisation, mobile phase modifiers). Exciting new technology (e.g. imaging, ion mobility, supercritical fluid), sample preparation (microextraction, molecular imprinted polymers) are advancing the field. Automated analysis of wider profiles of steroids is within reach, in smaller and more varied sample types, including molecular mapping of tissue sections. These new analytical dimensions require rigorous validation and advanced statistics. This review highlights that MS continues to open new windows of biochemical understanding in endocrinology.

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Section: What Lies Beneathmentioning

confidence: 99%

Mass spectrometry: Future opportunities for profiling and imaging steroids and steroid metabolites

Andrew

Homer

2020

Current Opinion in Endocrine and Metabolic Research

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“…Simple buffered/SDS solutions [26,28] further modified by organic solvents [19,25,27,31,32], bile salts [23,29,32,33,34], or CDs of diverse functionalities [19,23,24,30,32,34,35] are the commonly used electrolyte systems. Detection of steroids is usually promoted by the direct UV absorbance mode at low wavelengths in regular uncoated capillaries [19,21,26,29] or z-shaped cells [24], although derivatization schemes have been reported [18]. The implementation of partial-filling strategies for the MEKC-ESI-MS determination of anabolic steroids has been demonstrated [33].…”

Section: Introductionmentioning

confidence: 99%

“…In fact, the determination of steroids, in both pharmaceutical preparations [13,14] and body fluids [15][16][17], represents one of the most rapidly growing application areas of CE. With exception of a few steroidal compounds with ionizable phenolic OH groups, the majority of steroids are neutral compounds and thus either EKC or CEC are the recommended CE modes for method development initiatives unless derivatization procedures for charge generation are performed [18].…”

Section: Introductionmentioning

confidence: 99%

Mixture‐designed electrolytes for the MEKC separation of natural and synthetic steroids

et al. 2007

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In this work, the separation of 11 natural and synthetic steroids was studied using MEKC electrolytes modified by property-selected organic solvents: ethanol, ACN, and THF. The interplay between electrophoretic behavior and structural features was disclosed and the effects of organic modifiers to modulate retention and to alter selectivity were discussed in terms of system linear solvation energy relationships (LSER). The LSER indicated the total organic solvent percentage in the electrolyte as a major parameter to control retention and as a minor contribution, the hydrogen bond acidity. By evaluating the electropherograms obtained from mixture-designed electrolytes, a favorable separation condition for all solutes was achieved in ca. 25 min with an electrolyte composed of 20 mmol/L sodium tetraborate at pH 9.4, 20 mmol/L SDS and 20% EtOH (0.80% CV for migration time and 2.5% CV for peak area, n = five consecutive injections). The applicability of the proposed separation condition was demonstrated by the inspection of estrogens in urine sample (puberty stage).

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“…ketosteroids with Girard P or T reagents is a solution for this problem: the Girard hydrazones of 4-ene-3-oxo-and 17-oxosteroids can easily be separated and quantificated. 197 Capillary electrophoresis can be a useful tool for the preconcentration of different classes of steroids, making use of electroosmotic flow and capillaries partially filled with various reagents, e.g., borate for the complexation of steroids with proximal hydroxyls, high pH buffers for weakly acidic estrogens, micelle forming agents for hydrophobic steroids. 198…”

Section: ·1 Capillary Electrophoresis (Ce)mentioning

confidence: 99%

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

Gǒrög¹

2004

ANAL. SCI.

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Analysis of steroids Part 50. Derivatization of ketosteroids for their separation and determination by capillary electrophoresis

Cited by 18 publications

References 11 publications

Mass spectrometry: Future opportunities for profiling and imaging steroids and steroid metabolites

Mass spectrometry: Future opportunities for profiling and imaging steroids and steroid metabolites

Mixture‐designed electrolytes for the MEKC separation of natural and synthetic steroids

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

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