Analysis of Stomata, Chloroplast, and Chromosome of Local Mandarin Citrus (Citrus reticulata) Plants Grown from Endosperm Culture

Abstract

This research aimed to identify accessions of jeruk keprok Garut plants (Citrus reticulata) grown from endosperm culture with stomata analysis, chloroplasts analysis, and chromosome analysis. This research was conducted at the Plant Breeding Laboratory of Indonesian Citrus and Subtropical Fruits Research Institute (ICSFRI), Malang. This research used seven accessions of jeruk keprok Garut (local mandarin citrus) from endosperm culture and mother plant of Garut mandarin. Data analysis was carried out with the Statistical Package for the Social Sciences (SPSS) application Application 22.0. The alignment analysis showed that all one accession (GT 224) out of seven accessions of Garut mandarin grown from the endosperm cultures is consistent with the mother plant according to chromosome number observation, stomata number observation, and chloroplast number observation.

The phenetic relationship of citrus plants based on the morphological and anatomical characteristics

“…Meanwhile, the lowest average number of stomata was found in chokun, 85.89, with a range of 73-94. The greater the number of stomata, the higher the stomata density and the closer the stomata are located to each other (Kosmiatin et al 2019). In this study, two groups of stomata number were found, a large number of stomata (101-200) and quite a lot (51-100).…”

“…The study showed that the highest average stomata length was found in fremont, 21.4 μm, with a range of 18.93-23.33 μm, while the lowest average stomata length was found in santang, 17.92 μm with a range of 17.91-18.25 μm. Stomata length is classified into 3 sizes: (i) Less long if the length of the stomata is <20 μm; (ii) Length if it has a length of 20-25 μm; and (iii) Very long size if the stomata length is >25 μm (Kosmiatin et al 2019). In this study, stomata length was catagerized into less long (<20 μm) and long (20-25 μm).…”