Analysis of Subgingival Plaque Ability to Stimulate Toll‐Like Receptor 2 and 4

Ziauddin, SM; Raudales, Jorge Luis Montenegro; Sato, Kasumi; Yoshioka, Hidenobu; Kaneko, Takashi; Yoshimura, Atsutoshi; Hara, Yoshitaka

doi:10.1902/jop.2016.150573

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…In the gingival epithelium, CTHRC1 was mainly expressed in the cytoplasm but not in the cell membrane or intercellular space, and some cells showed strong positive expression in the cytoplasm or nuclear membrane. According to the expression locations, it was speculated that CTHRC1 might not be involved in the recognition of bacterial stimuli during the early stages of inflammation, because signal‐receiving proteins involved in the early stages of inflammation are usually expressed in cell membranes or interstitial spaces (e.g., CD14 and toll‐like receptors (TLRs)), and upon the bacterial attack on local tissues, the related protein receptors activate and rapidly receive external stimuli and then transmit the signals to cells, promoting the expression of inflammation‐related factors and immune responses (Magán‐Fernández et al., 2019; Ziauddin et al., 2016). Therefore, we presume that CTHRC1 might be one of the inflammatory cytokines involved in histological changes in inflamed gingiva epithelium.…”

Section: Discussionmentioning

confidence: 99%

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CTHRC1 expressed in periodontitis and human periodontal fibroblasts exposed to inflammatory stimuli

Huang

Guan

2022

Oral Diseases

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Objectives Collagen triple helix repeat containing‐1 (CTHRC1) is a glycoprotein that can be secreted extracellularly and is involved in the regulation of collagen matrix in a variety of diseases. The expression level of CTHRC1 in periodontitis was detected in this study. Materials and Methods The gingival tissues from clinically healthy subjects (15 cases) and those with periodontitis (30 cases) were taken for immunohistochemical staining. Lipopolysaccharide of the Porphyromonas gingivalis was added in the periodontal ligament fibroblast culture in vitro. Cells were collected, and the mRNA levels of the intracellular CTHRC1 and protein expression of the extracellular CTHRC1 were detected. Results The protein expression of CTHRC1 in the periodontitis group was higher than that of the clinically healthy group. The in vitro cell experiments showed that 10 μg/ml of P.g LPS could induce a significant increase in protein secretion of CTHRC1, and 5 μg/ml P.g LPS had a significant effect on promoting the mRNA expression of CTHRC1. Conclusions Collagen triple helix repeat containing‐1 might be involved in the development of periodontitis, and the expression level might be significantly correlated with the stimulation of P.g LPS on fibroblasts. Different stimulation intensities of P.g LPS might result in different expression patterns of CTHRC1.

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Section: Discussionmentioning

confidence: 99%

“…This study showed that CTHRC1 expression level increased significantly in inflammatory human gingiva than it did in controls. In F I G U R E 4 Primary human PDLFs (a1 and a2) were identified that they came from mesenchyme using the immunofluorescence labeling method (b1 and b2) responses (Magán-Fernández et al, 2019;Ziauddin et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

CTHRC1 expressed in periodontitis and human periodontal fibroblasts exposed to inflammatory stimuli

Huang

Guan

2022

Oral Diseases

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Expression of osteoclastogenic and anti-osteoclastogenic cytokines differs in mouse gingiva injected with lipopolysaccharide, peptidoglycan, or both

Kishimoto

Yamashita

Kaneko

et al. 2021

Archives of Oral Biology

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Porphyromonas gingivalis Mfa1 Induces Chemokine and Cell Adhesion Molecules in Mouse Gingival Fibroblasts via Toll-Like Receptors

Takayanagi

Kikuchi

Hasegawa

et al. 2020

JCM

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Porphyromonas gingivalis Mfa1 fimbriae are thought to act as adhesion factors and to direct periodontal tissue destruction but their immunomodulatory actions are poorly understood. Here, we investigated the effect of Mfa1 stimulation on the immune and metabolic mechanisms of gingival fibroblasts from periodontal connective tissue. We also determined the role of Toll-like receptor (TLR) 2 and TLR4 in Mfa1 recognition. Mfa1 increased the expression of genes encoding chemokine (C-X-C motif) ligand (CXCL) 1, CXCL3, intercellular adhesion molecule (ICAM) 1 and Selectin endothelium (E) in gingival fibroblasts, but did not have a significant effect on genes that regulate metabolism. Mfa1-stimulated up-regulation of genes was significantly suppressed in Tlr4 siRNA-transfected cells compared with that in control siRNA-transfected cells, which indicates that recognition by TLR4 is essential for immunomodulation by Mfa1. Additionally, suppression of Tlr2 expression partially attenuated the stimulatory effect of Mfa1. Overall, these results help explain the involvement of P. gingivalis Mfa1 fimbriae in the progression of periodontal disease.

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Analysis of Subgingival Plaque Ability to Stimulate Toll‐Like Receptor 2 and 4

Abstract: TLR4- but not TLR2-stimulating ability of SBP is associated with PI. Enhanced TLR4-stimulating ability at sites with accumulated plaque may mediate gingival inflammation.

Cited by 3 publications

References 26 publications

CTHRC1 expressed in periodontitis and human periodontal fibroblasts exposed to inflammatory stimuli

CTHRC1 expressed in periodontitis and human periodontal fibroblasts exposed to inflammatory stimuli

Expression of osteoclastogenic and anti-osteoclastogenic cytokines differs in mouse gingiva injected with lipopolysaccharide, peptidoglycan, or both

Porphyromonas gingivalis Mfa1 Induces Chemokine and Cell Adhesion Molecules in Mouse Gingival Fibroblasts via Toll-Like Receptors

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