Analysis of Sulfonamide Residues in Real Honey Samples Using Liquid Chromatography With Fluorescence and Tandem Mass Spectrometry Detection

Development and validation of a multiclass method for the determination of antibiotic residues in honey using liquid chromatography-tandem mass spectrometry

“…For that reason, it is necessary to include an pretreatment hydrolysis step to break the sugar-sulfonamide bond. Studies have demonstrated that methanol (Bernal, Nozal et al 2009) and hydrochloric acid (Thompson and Noot 2005;Sajid, Na et al 2013;Tölgyesi, Berky et al 2013;Dubreil-Chéneau, Pirotais et al 2014) were the main reagents used to hydrolyze N-glycoside bonds in order to give better recovery.…”

Simultaneous determination of eight corticosteroids in bovine tissues using liquid chromatography–tandem mass spectrometry

“…The recovery varied from 82% to 112%, and the reproducibility was between 4.7% and 27.6%. Reproducibility results can be accepted up to 30% at these levels [31].…”

“…Recently, we have demonstrated that the pH is one main parameter to reduce the matrix compounds during the sample cleanup [10,13,29,30]. The corticosteroids are non-polar and neutral molecules, however, matrix compounds generally can be ionized in the sample extracts depending on pH, therefore mixed-mode ion exchange SPE cartridges can be chosen for sample cleanup and concentration.…”

Quantification of T-2 and HT-2 mycotoxins in cereals by liquid chromatography-multimode ionization-tandem mass spectrometry

“…Generally, the advantages of core-shell type column compared to fully porous ones lie in their better resolution and sensitivity caused by the increased mass transfer, reduced longitudinal diffusion and more favorable eddy dispersion [31]. Kinetex XB column has been successfully applied for the separation of basic molecules (lincomycin, nitroimidazoles, sulfonamides, trimethoprim) in food and feed matrices [27,29]. In this study we tested this column for T-2 and HT-2 that do not contain acidic or basic functional groups (Fig.…”

“…This ion source contains both ESI and APCI parts, and therefore, its geometry radically differs to adequate ones [23]. Significantly, LC-MMI-MS/MS methods require thorough optimizations to reach good sensitivity for target compounds and to detect such low levels set by EU [24,[26][27][28][29][30].…”