Analysis of surface proteins of rat spermatozoa during epididymal transit and identification of antigens common to spermatozoa, rete testis fluid and cauda epididymal plasma

Brooks, D. E.; Tiver, K.

doi:10.1530/jrf.0.0710249

Cited by 36 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The transient appearance of CRES protein in the caput epididymidis implies that it is also important for early maturational events. Although many epididymal secretory proteins have been found to be synthesized specifically by the caput epithelium (Cameo and Blaquier, 1976;Vernon et al, 1982;Brooks and Tiver, 1984;Brooks, 1985;Jimenez et al, 1990;Rankin et al, 1992) CRES protein is quite different from those previously described in that its disappearance from the epididymis also occurs in the Mouse epididymal tissue and luminal fluid proteins were isolated from epididymal region 1 = proximal caput; 2 = midcaput; 3 = distal caput; 4 = corpus; and 5 = cauda epididymidis. B.…”

Section: +14-mentioning

confidence: 72%

Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation

Cornwall

Hann

1995

Molecular Reproduction Devel

View full text Add to dashboard Cite

In previous studies we identified an epididymal gene that exhibits homology to the cystatin family of cysteine protease inhibitors. The expression of this gene, termed CRES (cystatin-related epididymal and spermatogenic), was shown to be highly restricted to the proximal caput epididymal epithelium with less expression in the testis and no expression in the 24 other tissues examined. In this report, studies were carried out to examine CRES gene expression in the testis as well as to characterize the CRES protein in the testis and epididymis. In situ hybridization experiments revealed that within the testis CRES gene expression is stage-specific during spermatogenesis and is exclusively expressed by the round spermatids of Stages VII-VIII and the early elongating spermatids of Stages IX and X. Immunohistochemical studies demonstrated that CRES protein was transiently expressed in both the testis and epididymis. Within the testis the protein was localized to the elongating spermatids, whereas within the epididymis CRES protein was exclusively synthesized by the proximal caput epithelium and then secreted into the lumen. Surprisingly, the secreted CRES protein had completely disappeared from the epididymal lumen by the distal caput epididymidis. Western blot analysis of testicular and epididymal proteins showed that the CRES antibody specifically recognized a predominant 19 kDa CRES protein and a less abundant 14 kDa form. These observations suggest that the CRES protein performs a specialized role during sperm development and maturation.

show abstract

Section: +14-mentioning

confidence: 72%

Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation

Cornwall

Hann

1995

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…Proteins secreted by epididymal epithelial cells interact with sperm [4][5][6] and may contribute to their acquisition of fertilizing capacity [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Epididymal Specificity and Androgen Regulation of Rat EP21

Ibrahim

Young

Fröhlich

2001

Biology of Reproduction

View full text Add to dashboard Cite

In primates, expression of the EP2 gene is androgen-dependent and epididymis-specific. EP2 mRNA expression was investigated in caput, corpus, and cauda regions of rat epididymis and in 15 other rat tissues. Polymerase chain reaction and Northern analyses showed that rat EP2 is expressed predominantly in the proximal caput epididymidis. EP2 mRNA expression was determined in proximal epididymides from castrated, sham-operated, and efferent duct-ligated rats. In castrated rats, EP2 mRNA decreased to <10% of that in sham-operated rats between Days 3 and 4 postcastration, demonstrating the androgen dependence of EP2 expression. In epididymides ligated unilaterally at the efferent ducts, EP2 mRNA levels were approximately equal to those in the unligated contralateral epididymides or in sham-operated rats, indicating that EP2 expression does not depend on testicular factors. In bilaterally castrated rats, immediate and delayed testosterone replacement showed the dependence of EP2 expression on circulating androgens. Injection of testosterone propionate (TP) on Days 0, 1, 2, and 3 postcastration maintained EP2 mRNA levels approximately equal to those in sham-operated rats. Starting at Day 4 postcastration, daily injection of TP for 7 days restored EP2 mRNA to approximately normal levels. These data indicate for the rat that EP2 is expressed specifically in the proximal caput epididymidis and that its expression depends on circulating androgens but not on testicular factors.

show abstract

“…To test this possibility, we have examined by means of indirect immunofluorescence (IIF), the localization of protein DE on spermatozoa after in vitro and in vivo capacitation as well as after incubation under conditions known to affect the AR. INTRODUCTION Several reports published over the last few years indicate that acquisition of surface epididymal proteins by specific regions of the sperm surface is an essential facet of maturation in most mammals, including man (Kohane et al, 1980b;Moore, 1981;Gonzalez Echeverria et al, 1982;Vernon et al, 1982;Brooks andTiver, 1984, Tezon et al, 1985). The role of epididymal proteins as mediators of sperm maturation has been supported by experiments in which the addition of epididymal proteins to immature sperm increases their zona pellucida binding and fertilizing ability (Orgebin-…”

mentioning

confidence: 99%

Redistribution of a rat sperm epididymal glycoprotein after in vitro and in vivo capacitation

Rochwerger

Cuasnicú

1992

Molecular Reproduction Devel

145

View full text Add to dashboard Cite

Rat epididymal glycoprotein DE (37 kDa) associates with the sperm surface during maturation and is localized over the dorsal region of the acrosome. In the present study we examine, by indirect immunofluorescence, the localization of DE after in vitro and in vivo capacitation. While 49% of sperm capacitated in vitro for 5 hr still presented fluorescence over the dorsal region, 51% showed labeling distributed over a domain that corresponds to the equatorial segment of the sperm head. This change in the localization of fluorescence was not associated with sperm deterioration or death and increased gradually as a function of capacitation time, reaching the maximum at 5 hr. The presence of labeling over the equatorial segment results from protein migration and cannot be induced by permeabilization, proteinase, or high ionic strength treatments. The omission of Ca2+ from the standard capacitation medium inhibited the relocalization of DE, and incubation with Ca2+ ionophore A23187 for induction of the acrosome reaction (AR) significantly raised the percentage of cells with DE localized over the equatorial region. Finally, while free and cumulus-associated spermatozoa recovered from the oviducts of in vivo inseminated females presented 15% and 21% of cells with redistribution respectively, all perivitelline (acrosome reacted) spermatozoa showed DE over the equatorial segment. These results indicate that epididymal protein DE migrates to the equatorial segment under in vitro and in vivo capacitating conditions and suggest a possible association between the redistribution of DE and the occurrence of the AR.

show abstract

Analysis of surface proteins of rat spermatozoa during epididymal transit and identification of antigens common to spermatozoa, rete testis fluid and cauda epididymal plasma

Abstract: Summary. Spermatozoa from the testis and cauda epididymidis of the rat were surface labelled with radioactive iodide, extracted with detergent, and the radioactive proteins separated by two-dimensional

Cited by 36 publications

References 34 publications

Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation

Transient appearance of CRES protein during spermatogenesis and caput epididymal sperm maturation

Epididymal Specificity and Androgen Regulation of Rat EP21

Redistribution of a rat sperm epididymal glycoprotein after in vitro and in vivo capacitation

Contact Info

Product

Resources

About