Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age

Pujol-Lereis, Luciana M.; Rabossi, Alejandro; Quesada-Allué, Luis A.

doi:10.1016/j.jinsphys.2014.10.015

Cited by 13 publications

(14 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In insects, low temperatures induce the expression of genes of the antioxidant system and genes related with the heat shock response [26,31,45]. MnSOD and HSP90 transcripts accumulation were increased in cold-stressed 8-day-old individuals, as expected ( figure 5b-d).…”

Section: Discussionsupporting

confidence: 79%

“…Secondly, we studied the expression of a gene set associated with stress responses: Vitellogenin (Vg), Mn-Superoxide Dismutase (MnSOD), Heat-shock 70 (HSP70), and Heat-shock 90 (HSP90) [26,30,31]. Figure 5a shows that Vg transcript levels significantly increased in 6-and 8-day-old individuals exposed to cold stress, compared with individuals grown at the control temperature (two-way ANOVA, 6 days old: figure 5a).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Abscisic acid enhances cold tolerance in honeybee larvae

et al. 2017

View full text Add to dashboard Cite

The natural composition of nutrients present in food is a key factor determining the immune function and stress responses in the honeybee (Apis mellifera). We previously demonstrated that a supplement of abscisic acid (ABA), a natural component of nectar, pollen, and honey, increases honeybee colony survival overwinter. Here we further explored the role of ABA in in vitro-reared larvae exposed to low temperatures. Four-day-old larvae (L4) exposed to 258C for 3 days showed lower survival rates and delayed development compared to individuals growing at a standard temperature (348C). Cold-stressed larvae maintained higher levels of ABA for longer than do larvae reared at 348C, suggesting a biological significance for ABA. Larvae fed with an ABA-supplemented diet completely prevent the low survival rate due to cold stress and accelerate adult emergence. ABA modulates the expression of genes involved in metabolic adjustments and stress responses: Hexamerin 70b, Insulin Receptor Substrate, Vitellogenin, and Heat Shock Proteins 70. AmLANCL2, the honeybee ABA receptor, is also regulated by cold stress and ABA. These results support a role for ABA increasing the tolerance of honeybee larvae to low temperatures through priming effects.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Abscisic acid enhances cold tolerance in honeybee larvae

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Relevant experimental data that reported ontogenic variations of insect cold resistance exist mostly for insect model species (e.g., Arias et al, 2011;Colinet et al, 2013;Jensen et al, 2007;Pujol-Lereis et al, 2014; for the review of older studies see Bowler and Terblanche, 2008). In univoltine holometabolous species, differences in coping with cold exposures among stages could reflect adaptive responses, as the development stages can experience different thermal conditions, with the overwintering stage being most often the most coldresistant one (Denlinger and Lee, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, thermal tolerance can substantially vary over the course of an insect's life, but ontogenetic variations in the responses to low temperatures have been poorly examined compared to heat resistance (see Bowler and Terblanche, 2008). The existing studies have focused on preimaginal development or adult ageing (Colinet et al, 2013;David et al, 1998;Halle et al, 2015;Pujol-Lereis et al, 2014), while studies that included measures on both preimaginal and adult stages are commonly focused on a few developmental stages and age classes (e.g., Arias et al, 2011, but see Jensen et al, 2007.…”

Section: Introductionmentioning

confidence: 99%

Studies on chill coma recovery in the ladybird, Harmonia axyridis: Ontogenetic profile, effect of repeated cold exposures, and capacity to predict winter survival

Knapp

Vernon

Renault

2018

Journal of Thermal Biology

View full text Add to dashboard Cite

The harlequin ladybird, Harmonia axyridis, is one of the most successful invasive insect species worldwide. We investigated whether (i) chill coma recovery time (CCRt) changes during the ontogenetic development of this species, (ii) CCRt varies in response to repeated cold shocks, and (iii) CCRt could be a good predictor of winter survival ability in adults. CCRt decreased during larval development, the lowest CCRt values were observed in teneral adults (one and four days old), and significantly higher values were observed for older adults (16 and 32 days old). Repeated cold shocks (two hours at -3 °C), interrupted by short (30 min) warm periods (22 °C) resulted in decreased CCRt after the second cold shock, probably depicting an acclimation response, but then CCRt increased with additional cold shocks, likely revealing the accumulation of chill injuries. The CCRt of pre-overwintering individuals was not correlated with their winter survival. This indicates that CCRt is not a reliable measure of cold tolerance in H. axyridis. However, this result could be partially affected by the experimental setup - the use of laboratory-reared individuals who experienced standardized conditions and thus the variability in CCRt of tested animals could be much lower than the variability present in nature. The substantial variation observed over the ontogenetic development of H. axyridis poses important methodological implications for future studies, as animals of the same stage/age should be compared with each other. The observed U-shaped response to repeated cold shocks indicates that the expectation of linearity between cold exposure and insect response is an oversimplification of real situations.

show abstract

“…In medfly, a considerable number of studies have focused on the effects of stressful conditions during the adult stage such as high density, radiation exposure and reduction of food nutrition value on both longevity and female reproduction (Carey et al, 2002(Carey et al, , 2001Carey, Liedo, & Vaupel, 1995). Moreover, the effects of cold stress on adult survival, behaviour, gene expression and lipid profiles have been recently elucidated (Pujol-Lereis, Fagali, Rabossi, Catala, & Quesada-Allue, 2016;Pujol-Lereis, Rabossi, & Quesada-Allue, 2014). Thermal conditions during pupal development on adult flight performance have been also explored recently (Esterhuizen et al, 2014).…”

mentioning

confidence: 99%

Differential response to larval crowding of a long‐ and a short‐lived medfly biotype

Diamantidis

Ioannou

Nakas

et al. 2019

J of Evolutionary Biology

View full text Add to dashboard Cite

Response of endophytic fruit fly species (Tephritidae) to larval crowding is a form of scramble competition that may affect important life history traits of adults, such as survival and reproduction. Recent empirical evidence demonstrates large differences in adult life history traits, especially longevity, among Mediterranean fruit fly (Ceratitis capitata; "medfly") biotypes obtained from different regions of the world. However, whether the evolution of long lifespan is associated with response to stress induced by larval crowding has not been fully elucidated. We investigated, under constant laboratory conditions, the response of a short‐ and a long‐lived medfly biotypes to stress induced by larval crowding. Survival and development of larvae and pupae and the size of resulting pupae were recorded. The lifespan and age‐specific egg production patterns of the obtained adults were recorded. Our findings reveal that increased larval density reduced immature survival (larvae and pupae) in the short‐lived biotype but had rather neutral effects on the longed‐lived one. Only larvae of the long‐lived biotype were capable of prolonging their developmental duration under the highest crowding regime to successfully pupate and emerge as adults. Response of emerging adults to larvae crowding conditions was similar in the two medfly biotypes. Those individuals emerging from high larval density regimes had reduced longevity and fecundity. Long‐lived biotype individuals, however, appeared to suffer a higher cost in longevity compared with the short‐lived one. The importance of our findings to understand the evolution of long lifespan is discussed.

show abstract

Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: Effects of population heterogeneity and age

Cited by 13 publications

References 56 publications

Abscisic acid enhances cold tolerance in honeybee larvae

Abscisic acid enhances cold tolerance in honeybee larvae

Studies on chill coma recovery in the ladybird, Harmonia axyridis: Ontogenetic profile, effect of repeated cold exposures, and capacity to predict winter survival

Differential response to larval crowding of a long‐ and a short‐lived medfly biotype

Contact Info

Product

Resources

About