Analysis of taxonomic and geographic patterns of Turkish Veronica orientalis using nuclear and plastid DNA and morphological data

Sonibare, Mubo A.; Armağan, Metin; Özgökçe, Fevzi; Yaprak, Ahmet Emre; Mayland‐Quellhorst, Eike; Albach, Dirk C.

doi:10.1007/s00606-013-0909-4

Cited by 8 publications

(13 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Veronica L. (Plantaginaceae) is a genus of about 450 species ( Albach and Meudt, 2010 ) occurring over most of the Northern Hemisphere and in many parts of the Southern Hemisphere ( Mabberley, 2008 ). Modern systematic studies of the genus have focused on molecular phylogenies of several nuclear and plastid markers: the nuclear ribosomal internal transcribed spacer, nrITS ( Wagstaff and Garnock-Jones, 1998 ; Albach and Chase, 2001 ; Albach et al, 2004 ; Buhk et al, 2015 ), the plastid regions trn L-F ( Albach et al, 2004 , 2006 ; Buhk et al, 2015 ), ndh F- rpl 32 ( Sonibare et al, 2014 ; Albach and Al-Gharaibeh, 2015 ; Buhk et al, 2015 ), trn H- psb A ( Sonibare et al, 2014 ; Albach and Al-Gharaibeh, 2015 ), rps 16 intron ( Albach and Chase, 2004 ), rps 16– trn K ( Sonibare et al, 2014 ; Albach and Al-Gharaibeh, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Veronica officinalis Product Authentication Using DNA Metabarcoding and HPLC-MS Reveals Widespread Adulteration with Veronica chamaedrys

et al. 2017

View full text Add to dashboard Cite

Studying herbal products derived from local and traditional knowledge and their value chains is one of the main challenges in ethnopharmacology. The majority of these products have a long history of use, but non-harmonized trade and differences in regulatory policies between countries impact their value chains and lead to concerns over product efficacy, safety and quality. Veronica officinalis L. (common speedwell), a member of Plantaginaceae family, has a long history of use in European traditional medicine, mainly in central eastern Europe and the Balkans. However, no specified control tests are available either to establish the quality of derived herbal products or for the discrimination of its most common substitute, V. chamaedrys L. (germander speedwell). In this study, we use DNA metabarcoding and high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) to authenticate sixteen V. officinalis herbal products and compare the potential of the two approaches to detect substitution, adulteration and the use of unreported constituents. HPLC-MS showed high resolution in detecting phytochemical target compounds, but did not enable detection of specific plant species in the products. DNA metabarcoding detected V. officinalis in only 15% of the products, whereas it detected V. chamaedrys in 62% of the products. The results confirm that DNA metabarcoding can be used to test for the presence of Veronica species, and detect substitution and/or admixture of other Veronica species, as well as simultaneously detect all other species present. Our results confirm that none of the herbal products contained exactly the species listed on the label, and all included substitutes, contaminants or fillers. This study highlights the need for authentication of raw herbals along the value chain of these products. An integrative methodology can assess both the quality of herbal products in terms of target compound concentrations and species composition, as well as admixture and substitution with other chemical compounds and plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Veronica officinalis Product Authentication Using DNA Metabarcoding and HPLC-MS Reveals Widespread Adulteration with Veronica chamaedrys

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Polyploidy is in fact rampant across the genus in both hemispheres, with 76% of all species of known ploidy being polyploid (http://onlinelibrary.wiley.com/doi/10.1111/boj.12276/suppinfo; 56% considering Northern Hemisphere species only), and estimates of 20–25% of all speciation events being associated with polyploidy (Albach et al ., ). Numerous studies have investigated some of these polyploid complexes and have highlighted the importance of detailed analyses for the distribution of particular ploidies (Martínez‐Ortega et al ., ; Albach, ; Bardy et al ., , ; Sonibare et al ., ). Although polyploidy occurs in almost all subgenera of Veronica , polyploid lineages have had mixed diversification success (http://onlinelibrary.wiley.com/doi/10.1111/boj.12276/suppinfo; see also table 3 from Albach et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…Veronica subgenus Pentasepalae (1Cx‐value = 0.28–0.92 pg; 3.29‐fold variation) comprises a large number of Asian and European dry meadow and alpine species, including many polyploid complexes (e.g. Martínez‐Ortega et al ., ; Sonibare et al ., ). With a diversification rate of 0.23 speciation events per million years, it exhibits the third fastest diversification rate in the genus (http://onlinelibrary.wiley.com/doi/10.1111/boj.12276/suppinfo).…”

Section: Discussionmentioning

confidence: 97%

“…However, this should be considered as tentative and needs to be further supported by chromosome numbers and genome size estimates made on the same individual. Veronica thymifolia , considered to be diploid based on chromosome counts, fits karyologically and geographically into the European lineage of the subgenus with its 1C‐value = 0.57 pg (1Cx‐value = 0.57 pg), but phylogenetically it belongs to the Asian species (Muñoz‐Centeno et al ., ; Sonibare et al ., ; http://onlinelibrary.wiley.com/doi/10.1111/boj.12276/suppinfo).…”

Section: Discussionmentioning

confidence: 99%

“…), we used a related species for the ITS sequence ( V. reuteriana Boiss .) and, in another case ( V. orientalis ), we used a related species for the trnL‐trnL‐trnF sequence ( V. oltensis Woronow ex Elenevsky) based on more in‐depth phylogenetic analyses of the respective group (Sonibare et al ., ; D. C. Albach & E. Mayland‐Quellhorst, unpubl. data).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Is genome downsizing associated with diversification in polyploid lineages ofVeronica?

et al. 2015

Self Cite

View full text Add to dashboard Cite

The study of genome size evolution in a phylogenetic context in related polyploid and diploid lineages can help us to understand the advantages and disadvantages of genome size changes and their effect on diversification. Here, we contribute 199 new DNA sequences and a nearly threefold increase in genome size estimates in polyploid and diploid Veronica (Plantaginaceae) (to 128 species, c. 30% of the genus) to provide a comprehensive baseline to explore the effect of genome size changes. We reconstructed internal transcribed spacer (ITS) and trnL‐trnL‐trnF phylogenetic trees and performed phylogenetic generalized least squares (PGLS), ancestral character state reconstruction, molecular dating and diversification analyses. Veronica 1C‐values range from 0.26 to 3.19 pg. Life history is significantly correlated with 1C‐value, whereas ploidy and chromosome number are strongly correlated with both 1C‐ and 1Cx‐values. The estimated ancestral Veronica 1Cx‐value is 0.65 pg, with significant genome downsizing in the polyploid Southern Hemisphere subgenus Pseudoveronica and two Northern Hemisphere subgenera, and significant genome upsizing in two diploid subgenera. These genomic downsizing events are accompanied by increased diversification rates, but a ‘core shift’ was only detected in the rate of subgenus Pseudoveronica. Polyploidy is important in the evolution of the genus, and a link between genome downsizing and polyploid diversification and species radiations is hypothesized. © 2015 The Linnean Society of London, Botanical Journal of the Linnean Society, 2015, 178, 243–266.

show abstract

Allopatric hybrids as evidence for past range dynamics in Sempervivum (Crassulaceae), a western Eurasian high mountain oreophyte

Klein

Kadereit

2016

Alp Botany

View full text Add to dashboard Cite

Analysis of taxonomic and geographic patterns of Turkish Veronica orientalis using nuclear and plastid DNA and morphological data

Cited by 8 publications

References 57 publications

Veronica officinalis Product Authentication Using DNA Metabarcoding and HPLC-MS Reveals Widespread Adulteration with Veronica chamaedrys

Veronica officinalis Product Authentication Using DNA Metabarcoding and HPLC-MS Reveals Widespread Adulteration with Veronica chamaedrys

Is genome downsizing associated with diversification in polyploid lineages ofVeronica?

Allopatric hybrids as evidence for past range dynamics in Sempervivum (Crassulaceae), a western Eurasian high mountain oreophyte

Contact Info

Product

Resources

About