Analysis of telomerase activity and detection of its catalytic subunit, hTERT

Saldanha, Sabita N.; Andrews, Lori B.; Tollefsbol, Trygve O.

doi:10.1016/s0003-2697(02)00663-2

Cited by 73 publications

(73 citation statements)

References 92 publications

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“…The source and the quality of the sample influence the result when telomerase is extracted from tissues or cells. 28 Both benign and malignant clinical cytological samples are often hemorrhagic, which leads to effects of interfering substances and inhibition (eg, erythrocytes, hemoglobin) of PCR. With the TRAP extract method this has been shown for fine-needle aspirates and for effusions.…”

Section: Sensitivity For Malignancy Of Trap In Situmentioning

confidence: 99%

Telomere repeat amplification protocol (TRAP) in situ reveals telomerase activity in three cell types in effusions: malignant cells, proliferative mesothelial cells, and lymphocytes

Zendehrokh

Dejmek

2005

Modern Pathology

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Telomerase Repeat Amplification Protocol (TRAP) in situ was performed on cytospin preparations from 65 effusions from the serous cavities (45 pleural and 19 ascitic fluids and one pericardial fluid) submitted for routine diagnosis and the results were correlated to cytological morphology. Three types of cells with nuclear fluorescence were identified: malignant cells, hyperplastic mesothelial cell and lymphocytes. Of 38 cytologically malignant effusions, 12 showed strong reactivity in all malignant cells, three strong reactivity in part of the malignant population, whereas 12 showed moderate reactivity in the whole and five in part of the malignant population, respectively. In five malignant effusions weak reactivity was found in all (one case) and in scattered (four cases) malignant cells. Two effusions contained telomerase-negative malignant cells. Two pleural and two ascitic fluids contained proliferative mesothelial cells with weak or, in one case, moderate reactivity. Lymphocytes usually showed weak telomerase activity. Telomerase was expressed in almost all malignant tumours metastatic to serous cavities. Heterogeneity in tumour populations was demonstrated, which may have diagnostic implications, especially in cytology. Weak or moderate reactivity was found in lymphocytes and in some mesothelial proliferations and may explain the low specificity for malignancy sometimes obtained with the TRAP extract method. The weak reactivity found in lymphocytes may reduce the specificity when the extract method is used but causes no diagnostic problem with the TRAP in situ method.

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Section: Sensitivity For Malignancy Of Trap In Situmentioning

confidence: 99%

Telomere repeat amplification protocol (TRAP) in situ reveals telomerase activity in three cell types in effusions: malignant cells, proliferative mesothelial cells, and lymphocytes

Zendehrokh

Dejmek

2005

Modern Pathology

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“…PCR step of the TRAP assay is a source of potential problems, hence several non-PCR methods are also used. One of the most promising alternative methods is isothermic transcription-mediated amplification (TMA) also combined with detection based on hybridization-protection assay (HPA) and telomeric repeat elongation (TRE) based on biosensor technology [38]. The sensitivity of telomerase activity assays may be increased by a simultaneous assessment of hTERT expression.…”

Section: Telomerase and Telomeres As A Diagnostic Marker In Postmenopmentioning

confidence: 99%

Telomerase as a target in diagnosis and treatment of cancer in postmenopausal women

Lipińska¹,

Rubiś

2013

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SummaryTelomeres and telomerase are responsible for protection and maintenance of genomic stability of all eukaryotic cells. Several studies showed that one of the most distinguishing features of cancer cells is an increased expression and activity of telomerase, in contrast to somatic cells. This gives an opportunity for use of telomerase as a specific marker of cancer lesions or even as a molecular target. However, there are some questions concerning diagnostic specificity of this marker especially in the context of high proliferation potential of normal cells, such as the uterus and ovaries. The proliferation potential, however, decreases due to the menopause syndrome. Women's age and hormonal changes influence the functioning of many tissues, including alterations in telomerase activity of endometrial and ovarian cancer cells. The different characteristics of telomerase activity and telomere length in cells of postmenopausal women give an opportunity to utilize the telomeres and telomerase for the development of new cancer diagnostic tests and therapeutic methods. This work is aimed to summarize the information about the significance of telomeres and telomerase in the diagnosis and treatment of cancer in postmenopausal women.Key words: telomerase, telomeres, ovarian cancer, endometrium, post-menopausal women. StreszczenieTelomery wraz z telomerazą odpowiadają za ochronę oraz utrzymanie stabilności genomu wszystkich komórek eukariotycznych. Liczne badania na ten temat wykazały, że szczególnymi cechami większości komórek nowotworowych są podwyższona ekspresja i aktywność telomerazy w porównaniu z komórkami somatycznymi. Stwarza to potencjalną możliwość zastosowania telomerazy jako specyficznego markera zmian nowotworowych, ale i celu terapii. W odniesieniu do nowotworów układu rozrodczego kobiet pojawiają się jednak wątpli-wości związane z wysokim potencjałem proliferacyjnym prawidłowych komórek budujących takie organy, jak macica czy jajniki; tym samym kwestionowana jest specyficzność wspomnianego markera. Potencjał ten jednak zanika wraz z wejściem kobiety w okres menopauzy. Wiek kobiety i zmiany hormonalne wpływają na funkcjonowanie wielu tkanek, zmienia się m.in. aktywność telomerazy w komórkach endometrium i jajników. Odmienna charakterystyka aktywności telomerazy oraz długość telomerów w komórkach kobiet w okresie pomenopauzalnym daje możliwość wykorzystania tych czynników do opracowywania nowych testów diagnostycznych i metod terapeutycznych. Poniższa praca ma na celu podsumowanie informacji dotyczących znaczenia telomerów i telomerazy w diagnostyce oraz terapii onkologicznej kobiet po menopauzie.Słowa kluczowe: telomeraza, telomery, rak jajnika, endometrium, kobiety po menopauzie. Telomerase as a target in diagnosis and treatment of cancer in postmenopausal womenZnaczenie telomerazy w diagnostyce i terapii onkologicznej u kobiet po menopauzie

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“…The increase in telomerase in cancer cells generally occurs very early during tumorigenesis and sensitive techniques such as the telomeric repeat amplification protocol (TRAP) assay can detect trace levels of this enzyme, a method that has obvious potential for cancer diagnosis (6). …”

Section: Importance Of Telomerase In Cancer and Agingmentioning

confidence: 99%

Methods of Telomerase Inhibition

Andrews

Tollefsbol

2007

Telomerase Inhibition

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SummaryTelomerase is central to cellular immortality and is a key component of most cancer cells although this enzyme is rarely expressed to significant levels in normal cells. Therefore, the inhibition of telomerase has garnered considerable attention as a possible anticancer approach. Many of the methods applied to telomerase inhibition focus on either of the two major components of the ribonucleoprotein holoenzyme, that is, the telomerase reverse transcriptase (TERT) catalytic subunit or the telomerase RNA (TR) component. Other protocols have been developed to target the proteins, such as tankyrase, that are associated with telomerase at the ends of chromosomes. This chapter summarizes some of these recent advances in telomerase inhibition.

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Analysis of telomerase activity and detection of its catalytic subunit, hTERT

Cited by 73 publications

References 92 publications

Telomere repeat amplification protocol (TRAP) in situ reveals telomerase activity in three cell types in effusions: malignant cells, proliferative mesothelial cells, and lymphocytes

Telomere repeat amplification protocol (TRAP) in situ reveals telomerase activity in three cell types in effusions: malignant cells, proliferative mesothelial cells, and lymphocytes

Telomerase as a target in diagnosis and treatment of cancer in postmenopausal women

Methods of Telomerase Inhibition

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