Analysis of the 19 Y-STR and 16 X-STR loci system in the Han population of Shandong province, China

Gao, Haidong; Wang, C.; Han, Shuyi; Xiao, Dongjie; Wang, Y.S.; Li, C.T.; Zhang, M.X.

doi:10.4238/gmr16019573

Cited by 15 publications

(7 citation statements)

References 27 publications

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“…This result was quite different with other populations, such as Austria (0.7322) 21 , Germany(0.7314) 22 and Argentinean (0.6340) 23 . However, populations from Asia also showed lower heterozygosity at this locus, such as Japanese (0.2660) 24 , Korean (0.2513) 25 and Shandong Han (0.3470) 26 . This suggested us that more population genetic data are required to optimize the loci selection for forensic purpose.…”

Section: Resultsmentioning

confidence: 99%

Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes

Jia

Liu

Fan

et al. 2021

Sci Rep

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X-chromosome short tandem repeat (X-STR) markers are a powerful complementary system used for paternity and forensic casework. This study presents the development and validation of a new highly efficient multiplex-fluorescent-labeled 19 X-STR typing system, including DXS10079, DXS101, DXS10135, DXS10162, DXS6795, DXS6800, DXS6803, DXS6807, DXS6809, DXS6810, DXS7133, DXS7423, DXS981, DXS9902, DXS9907, GATA165B12, GATA172D05, GATA31E08 and HPRTB along with sex-typing locus, amelogenin. The system was validated according to guidelines issued by the Scientific Working Group on DNA Analysis Methods. Allele frequency and forensic parameters were investigated from 1085 (494 males and 591 females) unrelated Beijing Han individuals, the combined power of discrimination by the 19 X-STR loci in females and males, as well as the combined mean exclusion chance in trios and duos, were 0.999999999999999995, 0.99999999995, 0.9999999995, and 0.9999996, respectively. The results demonstrate that this multiplex system is robust and reliable, and considered to be a powerful tool for forensic application.

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Section: Resultsmentioning

confidence: 99%

Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes

Jia

Liu

Fan

et al. 2021

Sci Rep

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“…Several other commercial multiplexes have also been described in the literature [44][45][46] with most of them are from China. Led by AGCU Sci-enTch Incorporation (Wuxi, China), Yang et al [46] developed and improvised the 19 X-STRs multiplex (AGCU X19 STR Kit) from the previous AGCU X12 STR system [47].…”

Section: Development Of X-strs Analysismentioning

confidence: 99%

“…Among others, the newly developed kit included 11 loci that were previously used in the Qiagen Investigator® Argus X-12 QS Kit. Next, the Goldeneye17X system (another multiplex developed by Peoplespot, China) was reportedly using 5 colour fluorescent labelling technology that had enabled the amplification of 16 X-STRs loci and a sex determining locus (Amelogenin) in a single reaction [45]. The same authors further assessed genetic polymorphism of the multiplex on the Han population of Shandong province, China.…”

Section: Development Of X-strs Analysismentioning

confidence: 99%

Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review

Mahat

Alwi²,

Salleh³

et al. 2023

J.Trop.Life.Science

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The forensic DNA profiling technique has tremendously contributed to forensic human identification, an important aspect in forensic investigations. In instances whereby comparison samples are unavailable, utilization of short tandem repeats of X chromosome (X-STRs) may prove useful to resolve complex kinship investigations involving missing persons and mass disasters. Despite such evidential values, the use of X-STRs during investigations remains scarce in many Southeast Asian countries including Malaysia, requiring concerted efforts for establishing forensic statistical support for its diverse populations (especially the admixture populations), standardizing core loci and procedure, improving the knowledge among practitioners as well as developing suitable standard operating procedure for incorporating X-STRs analysis in the overall DNA profiling framework. Hence, this review paper aims to highlight the developments, applications and population data of X-STRs, as well as its challenges and future insights for forensic casework.

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“…The Y-chromosome acts as a unique tool for forensic investigations since it is inherited through the patrilineal line in a relatively conserved manner. A number of commercial Y-STR kits are available and most incorporate 19-36 markers into the single multiplex systems, which have been validated for casework [2,3]. Despite the robustness of these commercial multiplex Y-STR systems and the ability to discriminate two male individuals in most cases, the coincidence match probabilities are modest compared with a set of standard autosomal STR markers.…”

Section: Introductionmentioning

confidence: 99%

Development of a new 17 Y-STRs system using fluorescent-labelled universal primers and its application in Shanxi population in China

Liu

Wang²,

Tang

et al. 2019

Forensic Science International: Genetics Supplement Series

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Y-chromosomal short tandem repeats (Y-STRs) polymorphisms are useful in forensic identification, population genetics and constructing of human structures. Increasing the number of Y-STRs and their polymorphism will drastically narrow down the matching number of genealogy populations or pedigrees when searching against a forensic DNA databank. In this study, we develop a system containing 17 complementary Y-STRs that are compatible and reinforce the current commercially available Y-STR kits. This system was constructed by multiplex PCR with expected size of 126bp-400bp using home-made universal primers labeled by different fluorescence (DYS715,

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Analysis of the 19 Y-STR and 16 X-STR loci system in the Han population of Shandong province, China

Cited by 15 publications

References 27 publications

Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes

Development and validation of a multiplex 19 X-chromosomal short tandem repeats typing system for forensic purposes

Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review

Development of a new 17 Y-STRs system using fluorescent-labelled universal primers and its application in Shanxi population in China

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