Analysis of the Anti-HIV-1 Activity of an Anti-p17-Derivative Peptide (P30–52) Monoclonal Antibody

Abstract

The object of this study was to examine the possibility of immunotherapy using anti-human immunodeficiency virus type 1 (HIV-1) p17-derivative peptide monoclonal antibody (MAb), namely MAb 8H10. Previously we established MAb 8H10, and further characterization revealed that it inhibited the viral multiplication of the HIV-1-infected MT-4 cells, and that the inhibitory mechanism related to the decrease of p17 DNA of the infected cells. In the present study, based on the assumption that Hybridoma 8H10 (Hyb 8H10) is a source of this MAb, we examined how Hyb 8H10 influences the infected cells when the two are co-cultured using the transwell or by mixed culture. Hyb 8H10 did not influence the cell growth or viability of MT-4 cells, and MAb 8H10 was transferred to the cluster dish containing the infected cells. Furthermore, Hyb 8H10 could produce MAb 8H10 even when co-cultured with the infected MT-4 cells. However, the infectivity of the supernatant of the infected cells was temporarily enhanced when Hyb 8H10 was co-cultured without MAb 8H10, though it gradually reduced according to the increase in MAb 8H10 produced by Hyb 8H10. Though there were some problems, MAb 8H10 proved to be a strong candidate for immunotherapy against HIV.

Unique Epitope of Bovine Immunodeficiency Virus Gag Protein Spans the Cleavage Site between p16 MA and p2L

“…The MAb was also shown to inhibit cleavage at the p17-p24 site. Another group (13) has shown that MAb to a p17-derived peptide alone inhibits infection of cells by HIV Gag. Taken together, the results suggest that MAbs to Gag cleavage sites in bovine lentiviruses may be further explored for their ability to interact with and/or inhibit different strains of lentiviruses.…”