Analysis of the applicability of molecular markers linked to the PVY extreme resistance geneRysto, and the identification of new markers

Cernák, István; Taller, János; Wolf, I.; Fehér, Enikő; Babinszky, Gergely Csaba; Alföldi, Zoltán Péter; Csanádi, Gyula; Polgár, Zsolt

doi:10.1556/abiol.59.2008.2.6

Cited by 16 publications

(23 citation statements)

References 13 publications

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“…Valkonen et al (2008) found that the markers linked to the Ry sto gene (previously identified by Flis et al 2005 andSong et al 2005) are suitable for the selection of potato genotypes carrying the Ry sto gene from independent accession of S. stoloniferum; however, in our experiments, the markers mentioned above-with one exception-have not proved suitable for selection. These markers could not be detected in our experimental plant material, or they proved to be monomorphic (Cernák et al 2008). Only the STM0003-111 marker reported by Song et al (2005) could be detected in these plants.…”

Section: Discussionmentioning

confidence: 58%

“…According to our previous results, the female parent, White Lady, carries the Ry sto gene in simplex form (Cernák et al 2008).…”

Section: Plant Materialsmentioning

confidence: 80%

“…By means of marker-assisted selection, the identification of resistant lines can be simplified and made more reliable (Solomon-Blackburn and Barker 2001), but this requires the detection of markers closely linked to the given trait. Previously we identified three random amplified polymorphic DNA (RAPD) markers linked to the Ry sto gene (Cernák et al 2008). In order to eliminate the reproducibility problems encountered when using RAPD markers, an attempt was made to transform them into sequence-characterized amplified region (SCAR) markers, which are better suited for selection.…”

Section: Introductionmentioning

confidence: 99%

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Development of a locus-specific marker and localization of the Rysto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome

et al. 2008

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As a result of many decades of potato breeding in Keszthely, Hungary, numerous varieties resistant to the pathogens attacking potato have been developed using wild Solanum species as resistance sources. In order to simplify the time-consuming breeding process, the present research project involved the development of a SCAR marker correlating with the Ry sto gene, originating from the Solanum stoloniferun species and conferring extreme resistance to the potato Y virus (PVY), using sequence information from a RAPD marker linked to the gene. The usefulness of the marker was tested on 21 varieties resistant or susceptible to PVY and it was found to provide efficient identification of genotypes carrying the Ry sto gene. Based on its linkage to a new chromosome-specific anchor marker (Cat-in2), identified by means of the intron-targeting method not previously used in potato research, the Ry sto gene was localized on chromosome XII.

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Section: Discussionmentioning

confidence: 58%

“…According to our previous results, the female parent, White Lady, carries the Ry sto gene in simplex form (Cernák et al 2008).…”

Section: Plant Materialsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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Development of a locus-specific marker and localization of the Rysto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome

et al. 2008

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“…It is also possible to tag specific genes related to environmental factors that could have useful applications, for example in molecular ecology. This is because IT uses primers based on allele sequences of functionally characterized genes, and thus specific banding patterns corresponding to plant phenotypes can be identified [103,104]. However, development of such markers depends on the availability of genomic databases with several target sequences for IT markers.…”

Section: Gene-targeted and Functional Markers (Gtms And Fms)mentioning

confidence: 99%

Advances in plant gene-targeted and functional markers: a review

et al. 2013

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Public genomic databases have provided new directions for molecular marker development and initiated a shift in the types of PCR-based techniques commonly used in plant science. Alongside commonly used arbitrarily amplified DNA markers, other methods have been developed. Targeted fingerprinting marker techniques are based on the well-established practices of arbitrarily amplified DNA methods, but employ novel methodological innovations such as the incorporation of gene or promoter elements in the primers. These markers provide good reproducibility and increased resolution by the concurrent incidence of dominant and co-dominant bands. Despite their promising features, these semi-random markers suffer from possible problems of collision and non-homology analogous to those found with randomly generated fingerprints. Transposable elements, present in abundance in plant genomes, may also be used to generate fingerprints. These markers provide increased genomic coverage by utilizing specific targeted sites and produce bands that mostly seem to be homologous. The biggest drawback with most of these techniques is that prior genomic information about retrotransposons is needed for primer design, prohibiting universal applications. Another class of recently developed methods exploits length polymorphism present in arrays of multi-copy gene families such as cytochrome P450 and β-tubulin genes to provide cross-species amplification and transferability. A specific class of marker makes use of common features of plant resistance genes to generate bands linked to a given phenotype, or to reveal genetic diversity. Conserved DNA-based strategies have limited genome coverage and may fail to reveal genetic diversity, while resistance genes may be under specific evolutionary selection. Markers may also be generated from functional and/or transcribed regions of the genome using different gene-targeting approaches coupled with the use of RNA information. Such techniques have the potential to generate phenotypically linked functional markers, especially when fingerprints are generated from the transcribed or expressed region of the genome. It is to be expected that these recently developed techniques will generate larger datasets, but their shortcomings should also be acknowledged and carefully investigated.

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“…Examples of MAS have been published with regard to Ry adg and/or Ry sto (Dalla Rizza et al 2006; Gebhardt et al 2006;Valkonen et al 2008; Ottoman et al 2009). Markers linked to Ry adg (Kasai et al 2000) and Ry sto (Cernák et al 2008) have been optimised and validated in diverse genetic backgrounds. However, there are some additional PVY-resistant potato cultivars that are of interest to breeders, but for which the underlying genes remain elusive.…”

Section: Introductionmentioning

confidence: 99%

Hypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX

et al. 2014

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Potato virus Y (PVY, Potyvirus) is the fifth most important plant virus worldwide in terms of economic and scientific impact. It infects members of the family Solanaceae and causes losses in potato, tomato, tobacco, pepper and petunia production. In potato and its wild relatives, two types of resistance genes against PVY have been identified. While Ry genes confer symptomless extreme resistance, Ny genes cause a hypersensitive response visible as local necrosis that may also be able to prevent the virus from spreading under certain environmental conditions. The potato cultivar Sárpo Mira originates from Hungary and is highly resistant to PVY, although the source of this resistance remains unknown. We show that cv. Sárpo Mira reacts with a hypersensitive response leading to necrosis after PVYNTN infection in detached leaf, whole plant and grafting assays. The hypersensitivity to PVYNTN segregated amongst 140 individuals of tetraploid progeny of cvs. Sárpo Mira × Maris Piper in a 1:1 ratio, indicating that it was conferred by a single, dominant gene in simplex. Moreover, we identified five DNA markers linked to this trait and located the underlying locus (Ny-Smira) to the long arm of potato chromosome IX. This position corresponds to the location of the Rychc and Ny-1 genes for PVY resistance. A simple PCR marker, located 1 cM from the Ny-Smira gene, can be recommended for selection of PVY-resistant progeny of cv. Sárpo Mira.

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Analysis of the applicability of molecular markers linked to the PVY extreme resistance geneRy_sto, and the identification of new markers

Cited by 16 publications

References 13 publications

Development of a locus-specific marker and localization of the Rysto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome

Development of a locus-specific marker and localization of the Rysto gene based on linkage to a catalase gene on chromosome XII in the tetraploid potato genome

Advances in plant gene-targeted and functional markers: a review

Hypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX

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