Analysis of the bacterial communities and endosymbionts of natural populations of Bemisia tabaci in several crop fields from Mexico semi-arid zone

Caamal-Chan, María Goretty; Loera-Muro, Abraham; Castellanos, Thelma; Julian, Aguilar-Martínez Carlos; David, Marfil-Santana Miguel; Barraza, Aarón

doi:10.1007/s13213-019-01483-6

Cited by 9 publications

(3 citation statements)

References 51 publications

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“…According to the methodology described by Caamal-Chan et al . [30], total DNA extraction was carried out with modifications. To samples in extraction buffer were added 0.2 % β-mercaptoethanol and 1 mg ml −1 Proteinase K and incubated at 55 °C for 1 h with occasional stirring.…”

Section: Methodsmentioning

confidence: 99%

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

et al. 2021

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Arid plant communities provide variable diets that can affect digestive microbial communities of free-foraging ruminants. Thus, we used next-generation sequencing of 16S and 18S rDNA to characterize microbial communities in the rumen (regurgitated digesta) and large intestine (faeces) and diet composition of lactating creole goats from five flocks grazing in native plant communities in the Sonoran Desert in the rainy season. The bacterial communities in the rumen and large intestine of the five flocks had similar alpha diversity (Chao1, Shannon, and Simpson indices). However, bacterial community compositions were different: a bacterial community dominated by Proteobacteria in the rumen transitioned to a community dominated by Firmicutes in the large intestine. Bacterial communities of rumen were similar across flocks; similarly occurred with large-intestine communities. Archaea had a minimum presence in the goat digestive tract. We detected phylum Basidiomycota, Ascomycota, and Apicomplexa as the main fungi and protozoa. Analyses suggested different diet compositions; forbs and grasses composed the bulk of plants in the rumen and forbs and shrubs in faeces. Therefore, lactating goats consuming different diets in the Sonoran Desert in the rainy season share a similar core bacterial community in the rumen and another in the large intestine and present low archaeal communities.

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Section: Methodsmentioning

confidence: 99%

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

et al. 2021

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“…A species of the genus Candidatus Portiera, Candidatus Portiera aleyrodidarum is an obligate parasite vectored by the cotton white y, Bemisia tabaci (Gennadius) and other white ies (Ayesha et al 2015). A survey of bacterial communities associated with B. tabaci showed the predominance of the phylum, Proteobacteria of which the genus "Candidatus Portiera" was the most predominant obligate endosymbiont (Goretty et al 2019). Many economically and medically important bacteria cannot be cultured on any known laboratory media maybe due to speci c metabolic requirements and symbiotic relationships.…”

Section: Discussionmentioning

confidence: 99%

Chrysophyllum albidum Deterioration Entails Decline in Pseudomonas and Increase in Acetobacter Abundance

Ogbuji

Ataga

Ukwu

et al. 2023

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Microorganisms inhabiting fruits can affect the quality of fruits during storage. Some of these organisms are beneficial while others maybe deleterious (pathogenic). This paper analyzed African star apple (Chrysophyllum albidum) microbiota to detect the bacterial and fungal communities using high-throughput sequencing (HTS) technology. Healthy and diseased fruits of C. albidum were obtained from Choba market in Port Harcourt, Rivers State, Nigeria. Bacterial and fungal DNA were extracted from the samples and subjected to 16S and 18S rRNA sequencing respectively. Metagenomic analyses of bacterial and fungal strains from the samples revealed total operational taxonomical units (OTUs) as 341 and 4366 respectively. Among bacteria, the phylum Proteobacteria was dominant while all identified fungi belong to the phylum Ascomycota. There was a significant reduction in the abundance of Pseudomonas in the diseased sample when compared to the healthy sample. Conversely, relative abundance of Acetobacter increased in the diseased sample compared to the healthy sample. The fungal genera, Acidomyces, Geosmithia and Magnaporthe were also obtained. Additionally, the bacterial genera, Candidatus Portiera, Blautia, Brevibacterium, Tetragenococcus and Acinetobacter which were present in healthy fruits were not present in the diseased sample. The current study has helped in recognizing the microbial community structure of healthy and diseased fruits of C. albidum. These findings can help predict microbial community structural dynamics involved in the spoilage of African star apple and thus how the spoilage can be prevented or controlled.

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“…We washed each insect pool three times with 70% (v/v) ethanol, decanting the supernatant to remove environmental contaminants before DNA extraction. After, the samples were processed as described by Caamal et al (2019) . The quality of the extracted DNA was assessed using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Bacterial communities of the psyllid pest Bactericera cockerelli (Hemiptera: Triozidae) Central haplotype of tomato crops cultivated at different locations of Mexico

Caamal-Chan,

Barraza,

Loera-Muro

et al. 2023

PeerJ

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Background The psyllid, Bactericera cockerelli, is an insect vector of ‘Candidatus Liberibacter’ causing “Zebra chip” disease that affects potato and other Solanaceae crops worldwide. In the present study, we analyzed the bacterial communities associated with the insect vector Bactericera cockerelli central haplotype of tomato crop fields in four regions from Mexico. Methods PCR was used to amplify the mitochondrial cytochrome oxidase I gene (mtCOI) and then analyze the single nucleotide polymorphisms (SNP) and phylogenetic analysis for haplotype identification of the isolated B. cockerelli. Moreover, we carried out the microbial diversity analysis of several B. cockerelli collected from four regions of Mexico through the NGS sequencing of 16S rRNA V3 region. Finally, Wolbachia was detected by the wsp gene PCR amplification, which is the B. cockerelli facultative symbiont. Also we were able to confirm the relationship with several Wolbachia strains by phylogenetic analysis. Results Our results pointed that B. cockerelli collected in the four locations from Mexico (Central Mexico: Queretaro, and Northern Mexico: Sinaloa, Coahuila, and Nuevo Leon) were identified, such as the central haplotype. Analyses of the parameters of the composition, relative abundance, and diversity (Shannon index: 1.328 ± 0.472; Simpson index 0.582 ± 0.167), showing a notably relatively few microbial species in B. cockerelli. Analyses identified various facultative symbionts, particularly the Wolbachia (Rickettsiales: Anaplasmataceae) with a relative abundance higher. In contrast, the genera of Sodalis and ‘Candidatus Carsonella’ (Gammaproteobacteria: Oceanospirillales: Halomonadaceae) were identified with a relatively low abundance. On the other hand, the relative abundance for the genus ‘Candidatus Liberibacter’ was higher only for some of the locations analyzed. PCR amplification of a fragment of the gene encoding a surface protein (wsp) of Wolbachia and phylogenetic analysis corroborated the presence of this bacterium in the central haplotype. Beta-diversity analysis revealed that the presence of the genus ‘Candidatus Liberibacter’ influences the microbiota structure of this psyllid species. Conclusions Our data support that the members with the highest representation in microbial community of B. cockerelli central haplotype, comprise their obligate symbiont, Carsonella, and facultative symbionts. We also found evidence that among the factors analyzed, the presence of the plant pathogen affects the structure and composition of the bacterial community associated with B. cockerelli.

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Analysis of the bacterial communities and endosymbionts of natural populations of Bemisia tabaci in several crop fields from Mexico semi-arid zone

Cited by 9 publications

References 51 publications

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities

Chrysophyllum albidum Deterioration Entails Decline in Pseudomonas and Increase in Acetobacter Abundance

Bacterial communities of the psyllid pest Bactericera cockerelli (Hemiptera: Triozidae) Central haplotype of tomato crops cultivated at different locations of Mexico

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