Analysis of the biochemical composition of biological tissue in vivo by the diffuse light scattering method

Saetchnikov, Vladimir A.; Tcherniavskaia, Elina A.

doi:10.1007/s10812-011-9415-z

Cited by 4 publications

(6 citation statements)

References 9 publications

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“…A novel emerging method for the label-free analysis of nanoparticles and biomolecules in liquid fluids using optical micro cavity resonance of whispering-gallery-type modes is being developed [9][10][11][12]. Several schemes of experimental realization of the method have been tested [13][14][15][16]. The most promising looks a scheme based on polymer microspheres fixed by adhesive on the evanescence wave coupling element [12].…”

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confidence: 99%

“…The sensitivity of the developed scheme has been tested by monitoring the whispering gallery mode spectral shift. Water solutions of ethanol, HCl, glucose, vitamin C and biotin have been used [13][14][15][16]. Particular efforts were made for an optimal geometry for micro resonance observation under extremely low power of tunable laser exciting resonance.…”

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confidence: 99%

“…Material of microsphere the most appropriate for microbial application was also under investigation. Resonance shifts of C reactive protein water solutions as well as albumin solutions in pure water and with HCl modelling blood have been investigated in developed experimental geometry [13][14][15][16]. Introducing controlled amount of nano particles (50 nm in diameter glass gel solution) into microsphere surrounding was accompanied also by correlative resonance shift.…”

mentioning

confidence: 99%

“…As input data the following parameters were used: normalized by free spectral range resonance shift of whispering-gallery modes and a relative efficiency of whispering-gallery modes excitation. The last value can be interpreted as the intensity of resonant spectrum integrated over the free spectral range and normalized by the intensity maximum within this free spectral range.We have used several schemes of experimental realization of the method [13][14][15][16]. Here the newer version of the scheme has been developed [12].…”

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confidence: 99%

“…We have used several schemes of experimental realization of the method [13][14][15][16]. Here the newer version of the scheme has been developed [12].…”

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confidence: 99%

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Diagnostics of biomedical agents by whispering gallery mode optical resonance based sensor

Saetchnikov

Tcherniavskaia

Saetchnikov

et al. 2013

2013 International Conference on Advanced Optoelectronics and Lasers (CAOL 2013)

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Experimental data on optical resonance spectra of whispering gallery modes of dielectric microspheres in antibiotic solutions under varied in wide range concentration are represented. Optical resonance was demonstrated could be detected at a laser power of less than 1 microwatt. Several antibiotics of different generations:Amoxicillin, Azithromycin, Cephazolin, Chloramphenicol, Levofloxacin, Lincomicin Benzylpenicillin, Riphampicon both in de-ionized water and physiological solution had been used for measurements. Both spectral shift and the structure of resonance spectra were of specific interest in this investigation. Drag identification has been performed by developed multilayer perceptron network. The network topology was designed included: a number of the hidden layers of multilayered perceptron, a number of neurons in each of layers, a method of training of a neural network, activation functions of layers, type and size of a deviation of the received values from required values. For a network training the method of the back propagation error in various modifications has been used. Input vectors correspond to 6 classes of biological substances under investigation. The result of classification was considered as positive when each of the region, representing a certain substance in a space: relative spectral shift of an optical resonance maxima -relative efficiency of excitation of WGM, was singly connected.Recently a number of evanescent wave optical sensors have been developed and used for label-free biomolecule detection in sensing systems with extremely high sensitivity [1-8]. A novel emerging method for the label-free analysis of nanoparticles and biomolecules in liquid fluids using optical micro cavity resonance of whispering-gallery-type modes is being developed [9][10][11][12]. Several schemes of experimental realization of the method have been tested [13][14][15][16]. The most promising looks a scheme based on polymer microspheres fixed by adhesive on the evanescence wave coupling element [12]. The sensitivity of the developed scheme has been tested by monitoring the whispering gallery mode spectral shift. Water solutions of ethanol, HCl, glucose, vitamin C and biotin have been used [13][14][15][16]. Particular efforts were made for an optimal geometry for micro resonance observation under extremely low power of tunable laser exciting resonance. It was demonstrated that optical resonance under optimal geometry could be detected under the laser power of less then 1 microwatt. Material of microsphere the most appropriate for microbial application was also under investigation. Resonance shifts of C reactive protein water solutions as well as albumin solutions in pure water and with HCl modelling blood have been investigated in developed experimental geometry [13][14][15][16]. Introducing controlled amount of nano particles (50 nm in diameter glass gel solution) into microsphere surrounding was accompanied also by correlative resonance shift. The most attention was concentrated on development of a techniqu...

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

“…We have used several schemes of experimental realization of the method [13][14][15][16]. Here the newer version of the scheme has been developed [12].…”

mentioning

confidence: 99%

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