Analysis of the Capabilities of the Programs Fiji, Iplab and Darfi in the Study of Dna Repair Abilities in the Cells of Patients With a Mosaic Form of Ataxia Telangiectasia

Abstract

Different programs are used for processing and analyzing of fluorescent images. However, some of these programs produce uncertain results due to their dependence on human factor. Here we made a comparative study of three programs used for fluorescent images analysis: IPLab, Fiji and DARFI. The programs were compared by the fluorescence intensity measurement quality and cell nucleus area detection. We also analyzed the quality of counting of foci in the cell nucleus, the determination of foci area and foci fluorescence intensity. Fluorescent images were obtained with laser microscope Zeiss Axiovert 200M after indirect immunofluorescence. The DNA repair study was performed on three cell lines from patients with ataxia-telangiectasia (AT). This disease is characterized by natural disruptions of the ATM kinase function. The study of the features of ATM kinase in DNA repair in patient cells is relevant not only in AT diagnostic, but also in the estimation of disease severity.