Analysis of the Cell Wall Loosening Proteins During Rose Flower Opening

Takahashi, Rei; Fujitani, C.; Yamaki, Shohei; Yamada, Kenichiro

doi:10.17660/actahortic.2007.755.66

Cited by 16 publications

(18 citation statements)

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“…These results are consistent with a previous study that showed a correlation between expansin and XTH mRNA expression levels and rose flower opening (Takahashi et al, 2007). In this research, we showed the contribution of expansin and XTH to flower opening in more depth by unveiling the trends of these protein accumulations using Western blot analysis.…”

Section: Discussionsupporting

confidence: 93%

“…First-strand cDNA was synthesized using a High Fidelity RNA PCR kit (Takara, Shiga, Japan) with an oligo dT-adaptor primer (Takara). PCR was performed with an Adapter Primer (Takara) and the forward primers that were previously designed for cDNA cloning for α-expansin or XTH from rose petals (Takahashi et al, 2007), using the first-strand cDNA described above as the PCR template. PCR products were cloned into a pT7Blue T-vector (Novagen, Madison, WI, USA) and sequenced by a Big Dye Terminator v3.1 cycle sequence kit (Applied Biosystems, Foster City, CA, USA) using an automatic DNA sequencer (3130-Avant Genetic Analyzer; Applied Biosystems).…”

Section: Cdna Cloning For α-Expansins and Xthsmentioning

confidence: 99%

“…They participate in loosening and rearranging the cell walls (Rose et al, 2002), and their expressions are detected in growing tissues and ripening fruits (Catalá et al, 2000;Rose and Bennett, 1999). In petals, expansins and XTHs have been identified in several species (Gookin et al, 2003;Harada et al, 2010;Takahashi et al, 2007), and the function of α-expansin was analyzed using a transgenic antisense approach in Petunia hybrida (Zenoni et al, 2004). The predominance of XTH activity was associated with the opening of sandersonia flowers (O'Donoghue et al, 2002) and carnation flowers (Harada et al, 2010); however, there are few reports of the protein levels of expansin and XTH during flower opening, although their mRNA expression patterns have been a focus in previous reports (Sane et al, 2007;Singh et al, 2011;Yamada et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Expression of mRNAs and Proteins Associated with Cell-wall-loosening during Eustoma Flower Opening

Ochiai

Matsumoto

Maesaka

et al. 2013

J. Japan. Soc. Hort. Sci.

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Flower opening is important for floricultural crops. The mechanisms flower opening associated with the expansion of petal cells were investigated in Eustoma grandiflorum (Raf.) Shinn. Eustoma petals showed marked changes in their fresh weight, shape, and color during flower opening. Concurrently, petal cell-wall extensibility increased. This suggests that petal growth through flower opening is mainly caused by cell expansion. Expansin and xyloglucan endotransglycosylase/hydrolase (XTH) are known as representative proteins that loosen cell walls in plants. Three expansins and one XTH gene were isolated from opening Eustoma petals. We monitored for the first changes in their protein abundance in growing petals by Western blot analysis using antibodies to specifically detect expansin or XTH. The accumulation of these proteins marked the highest amount in petals when the flower was blooming and the petals were bending outwards. Thus, we showed that expansins participate in continuous petal growth from bud to opening flower and XTH plays a role in rapid petal growth accompanied by dynamic changes in petal fresh weight and petal shape.

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Section: Discussionsupporting

confidence: 93%

Section: Cdna Cloning For α-Expansins and Xthsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of mRNAs and Proteins Associated with Cell-wall-loosening during Eustoma Flower Opening

Ochiai

Matsumoto

Maesaka

et al. 2013

J. Japan. Soc. Hort. Sci.

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“…While creep extension analysis in the present study did not directly show that expansin and XTH led to rose petal expansion, the literature and present creep analysis indicated that these cell-wall-loosening proteins merited investigation in rose flowers with a view to elucidating their relation to petal growth. Earlier, we cloned three cDNAs of expansin (DDBJ accession numbers AB370116, AB370117, AB370118) and four cDNAs of XTH (DDBJ accession numbers AB428378, AB428379, AB428380, AB428381) from rose petals (Takahashi et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Also, the function of α-expansin was characterized using a transgenic antisense approach in Petunia hybrida (Zenoni et al, 2004). Previously, we reported the cloning of three α-expansin cDNAs and four XTH cDNAs from rose petals (Takahashi et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening

Yamada

Takahashi

Fujitani

et al. 2009

J. Japan. Soc. Hort. Sci.

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RhEXPA4, a rose expansin gene, modulates leaf growth and confers drought and salt tolerance to Arabidopsis

Lü

Mei

Jiang

et al. 2013

Planta

156

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Drought and high salinity are major environmental conditions limiting plant growth and development. Expansin is a cell-wall-loosening protein known to disrupt hydrogen bonds between xyloglucan and cellulose microfibrils. The expression of expansin increases in plants under various abiotic stresses, and plays an important role in adaptation to these stresses. We aimed to investigate the role of the RhEXPA4, a rose expansin gene, in response to abiotic stresses through its overexpression analysis in Arabidopsis. In transgenic Arabidopsis harboring the Pro RhEXPA4 ::GUS construct, RhEXPA4 promoter activity was induced by abscisic acid (ABA), drought and salt, particularly in zones of active growth. Transgenic lines with higher RhEXPA4 level developed compact phenotypes with shorter stems, curly leaves and compact inflorescences, while the lines with relatively lower RhEXPA4 expression showed normal phenotypes, similar to the wild type (WT). The germination percentage of transgenic Arabidopsis seeds was higher than that of WT seeds under salt stress and ABA treatments. Transgenic plants showed enhanced tolerance to drought and salt stresses: they displayed higher survival rates after drought, and exhibited more lateral roots and higher content of leaf chlorophyll a under salt stress. Moreover, high-level RhEXPA4 overexpressors have multiple modifications in leaf blade epidermal structure, such as smaller, compact cells, fewer stomata and midvein vascular patterning in leaves, which provides them with more tolerance to abiotic stresses compared to mild overexpressors and the WT. Collectively, our results suggest that RhEXPA4, a cell-wall-loosening protein, confers tolerance to abiotic stresses through modifying cell expansion and plant development in Arabidopsis.

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Analysis of the Cell Wall Loosening Proteins During Rose Flower Opening

Cited by 16 publications

References 0 publications

Expression of mRNAs and Proteins Associated with Cell-wall-loosening during Eustoma Flower Opening

Expression of mRNAs and Proteins Associated with Cell-wall-loosening during Eustoma Flower Opening

Cell Wall Extensibility and Effect of Cell-Wall-Loosening Proteins during Rose Flower Opening

RhEXPA4, a rose expansin gene, modulates leaf growth and confers drought and salt tolerance to Arabidopsis

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