Analysis of the Chemical Composition of the Essential Oil of Polygonum minus Huds. Using Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GC-TOF MS)

Abstract

The essential oil in leaves of Polygonum minus Huds., a local aromatic plant, were identified by a pipeline of gas chromatography (GC) techniques coupled with mass-spectrometry (MS), flame ionization detector (FID) and two dimensional gas chromatography time of flight mass spectrometry (GC×GC–TOF MS). A total of 48 compounds with a good match and high probability values were identified using this technique. Meanwhile, 42 compounds were successfully identified in this study using GC-MS, a significantly larger number than in previous studies. GC-FID was used in determining the retention indices of chemical components in P. minus essential oil. The result also showed the efficiency and reliability were greatly improved when chemometric methods and retention indices were used in identification and quantification of chemical components in plant essential oil.

Overexpressing 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGR) in the Lactococcal Mevalonate Pathway for Heterologous Plant Sesquiterpene Production

“…Whether this is the genuine product of the PMSTS isolated from P. minor , caused by the single amino acid substitution (K266E) introduced during the cloning process or an effect of the lactococcal host expressing the recombinant protein is yet to be resolved. It should also be noted that β-sesquiphellandrene was not detected in the essential oil of the P. minor plant itself, although all the other minor products detected from the in vitro assay of PMSTS was detected in the plant [24]. This further supports the hypothesis that the mutation or host may have affected the major product produced by PMSTS although it could also be argued that the extraction or detection method used by Baharom et al [24] was not sensitive towards β-sesquiphellandrene detection.…”

“…It should also be noted that β-sesquiphellandrene was not detected in the essential oil of the P. minor plant itself, although all the other minor products detected from the in vitro assay of PMSTS was detected in the plant [24]. This further supports the hypothesis that the mutation or host may have affected the major product produced by PMSTS although it could also be argued that the extraction or detection method used by Baharom et al [24] was not sensitive towards β-sesquiphellandrene detection. As can be seen from their study, analysis using GC-MS and GCxGC-TOF MS produced significant differences in the products detected in the essential oil of P. minor .…”

Evaluación de la acción antiséptica de un jabón líquido utilizando algunos aceites esenciales como agente activo

“…Para determinar las especificaciones de calidad de la formulación se establecieron, por triplicado y a tres muestras diferentes, elaboradas bajo las mismas condiciones, las características organolépticas como color, olor, así como sus características físicas y químicas como pH, viscosidad y composición química por cromatografía de gases/ espectrómetro de masa (GC/MS); igualmente, se determinó la actividad antioxidante in vitro del producto terminado [14][15][16][17][18][19][20].…”

“…Las condiciones de operación fueron las siguientes: columna capilar HP-5MS (5% phenyl methyl silox, 30 m × 250 m × 0,25 m), temperatura inicial 45 °C, temperatura de la línea de transferencia de 280 °C y volumen de inyección 1,0 L en modo split (20:1), con temperatura del inyector de 250 °C [13][14][15][16][17][18]. La detección de los compuestos se realizó por comparación del espectro de masas con los reportados en la base de datos NIST-2008 [13,14,19,20].…”

Metabolite profiling reveals temperature effects on the VOCs and flavonoids of different plant populations

“…Essential oil study using gas chromatography‐mass spectrometry (GC‐MS), GCxGC‐MS‐TOF (time‐of‐flight) and GC with flame ionisation detector (GC‐FID) successfully detected about 42 compounds, mainly aldehydes and terpenes (Baharum et al . ). Biological activity assays on the essential oils extracted from different tissues of P. minus showed potent antioxidant and antimicrobial activity against MRSA bacteria (Ahmad et al .…”