Analysis of the Clinical and Epidemiological Meaning of Screening Test for SARS-CoV-2: Considerations in the Chronic Kidney Disease Patients during the COVID-19 Pandemic

Martino, Francesca; Amici, Gianpaolo; Grandesso, Stefano; Mortellaro, Rosella Ferraro; Cicero, Antonina Lo; Novara, Giacomo

doi:10.3390/jcm10051139

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…Furthermore, the commercially available diagnostics kits in RT-qPCR have different features, mainly connected to the viral target tested and the limit of detection. Significantly, the higher the limit of detection, the more risk of false negatives [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding

Diego

Fernández‐Soto

Muñoz‐Bellido

et al. 2021

JCM

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Detection of SARS-CoV-2 is routinely performed in naso/oropharyngeal swabs samples from patients via RT-qPCR. The RT-LAMP technology has also been used for viral RNA detection in respiratory specimens with both high sensitivity and specificity. Recently, we developed a novel RT-LAMP test for SARS-CoV-2 RNA detection in nasopharyngeal swab specimens (named, N15-RT-LAMP) that can be performed as a single-tube colorimetric method, in a real-time platform, and as dry-LAMP. To date, there has been very little success in detecting SARS-CoV-2 RNA in urine by RT-qPCR, and the information regarding urine viral excretion is still scarce and not comprehensive. Here, we tested our N15-RT-LAMP on the urine of 300 patients admitted to the Hospital of Salamanca, Spain with clinical suspicion of COVID-19, who had a nasopharyngeal swab RT-qPCR-positive (n = 100), negative (n = 100), and positive with disease recovery (n = 100) result. The positive group was also tested by RT-qPCR for comparison to N15-RT-LAMP. Only a 4% positivity rate was found in the positive group via colorimetric N15-RT-LAMP and 2% via RT-qPCR. Our results are consistent with those obtained in other studies that the presence of SARS-CoV-2 RNA in urine is a very rare finding. The absence of SARS-CoV-2 RNA in urine in the recovered patients might suggest that the urinary route is very rarely used for viral particle clearance.

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Section: Discussionmentioning

confidence: 99%

Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding

Diego

Fernández‐Soto

Muñoz‐Bellido

et al. 2021

JCM

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“…In the coronavirus pandemic, managing the conditions of patients with either chronic kidney diseases or acute kidney injuries and kidney transplant patients undergoing immunosuppressive therapy proves a clinical challenge for nephrologists, particularly in patients with severe COVID-19. Under such circumstances, efficient management is necessary to mitigate side effects and drug interactions due to renal failure [ 15 , 16 ], given the absence of specific anti-COVID-19 treatment programs. Efficient management of these patients demands markers that are both effortlessly measurable and can help predict the condition of these patients.…”

Section: Discussionmentioning

confidence: 99%

The relationship between CT value and clinical outcomes in renal patients with COVID-19

et al. 2022

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Purpose Concomitant kidney diseases raise the mortality rate due to the SARS-CoV-2 virus as an independent factor. Although a qualitative PCR test’s result is sufficient for diagnosis, Cycle threshold value may present relevant information to the physicians in providing faster treatment in patients with chronic conditions, including kidney diseases, to prevent morbidity and subsequent mortality. Thus, the present study was conducted to determine the relationship between the Cycle threshold value and clinical outcomes in renal patients with the coronavirus 2019. Methods This retrospective study was conducted on renal patients with the coronavirus 2019 infection admitted to Labbafinejad Hospital in Tehran, the capital of Iran, within a period of one year, from late February 2020 to February 2021. Data were collected per the prepared checklist. Cycle threshold values were measured by performing PCR on nasopharynx and oropharynx swab samples of patients. Results According to the adjusted analysis, having high viral load increased the odds of in-hospital mortality (aOR = 11.65, 95% CI 3.93–34.54), ICU admission (aOR = 5.49, 95% CI 2.16–13.97), and invasive ventilation (aOR = 7.18, 95% CI 2.61–19.74). Having high viral load also increased the odds of O 2 therapy (aOR = 3.08, 95% CI 0.79–12.01), although the difference was not statistically significant ( P = 0.105). Conclusion Cycle threshold value was a significant predictor of mortality in renal patients. Nevertheless, further studies are required on how to render optimal use of the Cycle threshold value, given that the quality of the test sample and the different groups of patients under study affect the effectiveness of this marker in predicting disease severity.

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“…Several RT-PCR protocols have been proposed following the guidelines of the WHO (World Health Organization) and KCDC (Korea Centers for Disease Control and Prevention), which recommend amplification of the E, N and RdRp genes. When the protocols do not use these genes, the recommendation is that all the researched genes be detected to confirm the result 20 .…”

Section: Methods For Detecting Covid-19mentioning

confidence: 99%

Main aspects and prophylatic measures of COVID-19 in Brazil

et al. 2021

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A partir do primeiro caso de COVID-19 no Brasil, o país se tornou o terceiro no mundo em números de casos e de óbitos. Apesar das medidas implantadas pelo governo, o número de infectados e de óbitos por COVID-19 continua aumentando e o país enfrenta vários outros problemas que inclui aspectos sociais e políticos, dificultando as medidas de contenção da pandemia. O presente estudo visou abordar as características gerais do SARS-Cov-2, bem como apontar os principais aspectos socioepidemiológico no Brasil, e tratamento da COVID 19. Foi realizada uma revisão de literatura para busca de artigos em Bases de dados PubMed, Scielo e Google Scholar até 06 de outubro de 2020. Os pacientes com COVID-19 podem ser assintomáticos, porém entre os sintomáticos a gravidade da doença está relacionada à idade e a condições médicas pré-existentes. Os pulmões são os órgãos mais afetados pelo vírus e por isso as manifestações respiratórias como tosse, falta de ar, produção de escarro, dor de garganta e congestão nasal são os sintomas mais associados à COVID-19 A transmissão do SARS-COV-2 entre os humanos ocorre principalmente por meio de gotículas respiratórias, mas também podem ocorrer por meio do contato com superfícies contaminadas. Testes de vacinas foram realizados aprovados pela Organização Mundial da Saúde (OMS). O Brasil se destaca em segunda posição mundial, com cinco vacinas aprovadas, Pfizer-BioNTech, Oxford-AstraZeneca, /CoronaVac (Sinovac), Janssen/Covishield.

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Analysis of the Clinical and Epidemiological Meaning of Screening Test for SARS-CoV-2: Considerations in the Chronic Kidney Disease Patients during the COVID-19 Pandemic

Cited by 4 publications

References 39 publications

Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding

Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding

The relationship between CT value and clinical outcomes in renal patients with COVID-19

Main aspects and prophylatic measures of COVID-19 in Brazil

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