Analysis of the common deletions in the mitochondrial DNA is a sensitive biomarker detecting direct and non-targeted cellular effects of low dose ionizing radiation

Schilling-Tóth, Boglárka; Sándor, Nikolett; Kis, Enikő; Kadhim, Munira; Sáfrány, Géza; Hegyesi, Hargita

doi:10.1016/j.mrfmmm.2011.07.018

Cited by 35 publications

(25 citation statements)

References 56 publications

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“…By analyzing common deletions in the mitochondrial genome [22] of bystander cells we found that GDF-15 silencing abrogated the bystander response when conditioned medium was transferred to recipient cells from 100 mGy irradiated donor cells. Bystander effects might be induced by several mechanisms, such as intercellular communications (gap junctions), phagocytosis of apoptotic bodies, as well as the release of various agents [37,38] into the cellular neighborhood, which we have studied here.…”

Section: Discussionmentioning

confidence: 98%

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Growth Differentiation Factor-15 (GDF-15) is a potential marker of radiation response and radiation sensitivity

Sándor

Schilling-Tóth

Kis

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Section: Discussionmentioning

confidence: 98%

“…Radiation-induced bystander effects were studied by the analysis of deletions in the mitochondrial genome using a quantitative real-time polymerase chain reaction (qRT-PCR) protocol as described [22]. Briefly, donor cells were seeded in 25 cm 2 culture plates at the density of 2 × 10 4 cells/cm 2 .…”

Section: Radiation-induced Bystander Effectsmentioning

confidence: 99%

Growth Differentiation Factor-15 (GDF-15) is a potential marker of radiation response and radiation sensitivity

Sándor

Schilling-Tóth

Kis

et al. 2015

Mutation Research/Genetic Toxicology and Environmental Mutagene

Self Cite

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“…Interestingly,it was also found that the stable CA rates increased with the cumulative exposure time, while no significance was detected in unstable CA rates and increased exposure time, which may be explained by the fact that stable CAs did not disappear with increase in exposure time, while unstable CAs disappeared with increased exposure time. IR generates ionising particles, which directly induce SSBs and DSBs or types of DNA damage, leading to accumulation of MN and CAs, mainly determined by the length of exposure and the equivalent dose of radiation (13,39,40) . It is known that the radiation dose equivalent in individuals is gradually accumulated and increases with prolonged exposure time, and inadequate repair of DNA damage increases the risk of MN and CAs (41) .…”

Section: Discussionmentioning

confidence: 99%

Effects of Ionising Radiation on Micronucleus Formation and Chromosomal Aberrations in Chinese Radiation Workers

Qin

Cao

Shen

et al. 2015

Radiat Prot Dosimetry

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This study is aimed to investigate the effects of ionising radiation (IR) on micronuclei (MN) formation and chromosome aberrations (CAs) in Chinese radiation workers. The study was conducted using peripheral blood lymphocytes from 1392 radiation workers from Public Hospitals of the city of Tangshan (the exposed group), and 143 healthy individuals as the control group. Fluorescence in situ hybridisation (FISH) was used to detect the unstable and stable nuclear CAs on metaphase. The MN assay was performed using the cytochalasin B method for cytokinesis-block. The MN and CA frequencies were significantly higher in the exposed group than in healthy controls (both p < 0.001). Examination of the incidence rates of MN and CA showed an increasing trend among workers in some occupations compared with the others (all p < 0.05). There were also significant differences in MN and CA rates among workers with different exposure times (all p < 0.05). Stable CA rates demonstrated an increased trend among workers with different exposure times (all p < 0.05), while no significance of unstable CA rates was found among workers with different exposure times (all p < 0.05). Importantly, the frequencies of CA and MN increased among different cumulative radiation dose groups (all p < 0.05). Correlation analysis showed that the frequencies of MN and CA were positively associated with the cumulative radiation dose. Longterm exposure to IR may have harmful effects on the health of radiation workers. The data obtained here show an increased risk of genetic instability that correlated with occupation, exposure time and equivalent dose among Chinese radiation workers.

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“…The increased accumulation of common deletion (CD) following exposure to ionizing radiation has been detected in various studies (148–150). The measurement of CD by quantitative (real-time) polymerase chain reaction (PCR) has been proposed as a sensitive marker to detect low levels of oxidative damage to the mtDNA (148).…”

Section: Molecular and Cellular Mechanisms Underlying The Observedmentioning

confidence: 99%

“…The measurement of CD by quantitative (real-time) polymerase chain reaction (PCR) has been proposed as a sensitive marker to detect low levels of oxidative damage to the mtDNA (148). An increased accumulation of the CD has been observed in several human fibroblast cell lines following exposure to doses as low as 0.1 Gy (150). Interestingly, increased accumulation of the CD was also observed in bystander cells, i.e., cells that were cultured in conditioned medium derived from 0.1 Gy-irradiated cells.…”

Section: Molecular and Cellular Mechanisms Underlying The Observedmentioning

confidence: 99%

Cardiovascular diseases related to ionizing radiation: The risk of low-dose exposure (Review)

Baselet

Rombouts

Benotmane

et al. 2016

International Journal of Molecular Medicine

118

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Traditionally, non-cancer diseases are not considered as health risks following exposure to low doses of ionizing radiation. Indeed, non-cancer diseases are classified as deterministic tissue reactions, which are characterized by a threshold dose. It is judged that below an absorbed dose of 100 mGy, no clinically relevant tissue damage occurs, forming the basis for the current radiation protection system concerning non-cancer effects. Recent epidemiological findings point, however, to an excess risk of non-cancer diseases following exposure to lower doses of ionizing radiation than was previously thought. The evidence is the most sound for cardiovascular disease (CVD) and cataract. Due to limited statistical power, the dose-risk relationship is undetermined below 0.5 Gy; however, if this relationship proves to be without a threshold, it may have considerable impact on current low-dose health risk estimates. In this review, we describe the CVD risk related to low doses of ionizing radiation, the clinical manifestation and the pathology of radiation-induced CVD, as well as the importance of the endothelium models in CVD research as a way forward to complement the epidemiological data with the underlying biological and molecular mechanisms.

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Analysis of the common deletions in the mitochondrial DNA is a sensitive biomarker detecting direct and non-targeted cellular effects of low dose ionizing radiation

Cited by 35 publications

References 56 publications

Growth Differentiation Factor-15 (GDF-15) is a potential marker of radiation response and radiation sensitivity

Growth Differentiation Factor-15 (GDF-15) is a potential marker of radiation response and radiation sensitivity

Effects of Ionising Radiation on Micronucleus Formation and Chromosomal Aberrations in Chinese Radiation Workers

Cardiovascular diseases related to ionizing radiation: The risk of low-dose exposure (Review)

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