Analysis of the Composition of Deinagkistrodon acutus Snake Venom Based on Proteomics, and Its Antithrombotic Activity and Toxicity Studies

Abstract

There is a strong correlation between the composition of Deinagkistrodon acutus venom proteins and their potential pharmacological effects. The proteomic analysis revealed 103 proteins identified through label-free proteomics from 30 different snake venom families. Phospholipase A2 (30.0%), snaclec (21.0%), antithrombin (17.8%), thrombin (8.1%) and metalloproteinases (4.2%) were the most abundant proteins. The main toxicity of Deinagkistrodon acutus venom is hematotoxicity and neurotoxicity, and it acts on the lung. Deinagkistrodon acutus venom may have anticoagulant and antithrombotic effects. In summary, the protein profile and related toxicity and pharmacological activity of Deinagkistrodon acutus venom from southwest China were put forward for the first time. In addition, we revealed the relationship between the main toxicity, pharmacological effects, and the protein components of snake venom.

Structural, biochemical characterization and molecular mechanism of Cerastokunin: A new Kunitz-type peptide with potential inhibition of thrombin, factor Xa and platelets

“…The mouse model was performed as described by [27,28] with male NMRI mice (20-25g), with four individuals in each group. Cerastokunin dissolved in sterile Phosphore buffer (1×M, pH 7.4) was administered intravenously at doses of 2, 4 and 6 mg/kg body weight.…”

Immunoprofiling of Equine Plasma against Deinagkistrodon acutus in Taiwan: Key to Understanding Differential Neutralization Potency in Immunized Horses

“…This venomous snake is also commonly known as the hundred-pace pit viper or five-pace snake, and its bite can lead to severe rapid-onset coagulopathy and thrombocytopenia in D. acutus envenomed victims. The symptoms of D. acutus envenomation are caused by two main components, metalloproteases and serine proteases, present in the venom of hundred-pace pit viper [ 6 , 7 , 8 ].…”

Proteomics and Metabolomics in Biomedicine

“…Non-human organisms have been analyzed by some authors, resulting in the identification of two proteins, glucose/ribitol dehydrogenase and 16.9 kDa class I heat shock protein 1, as novel wheat allergens [37], or the obtainment of insights into triterpene saponin biosynthesis in the tea plant (Camellia sinensis) [38]. Moreover, the effects of different molecules have been investigated, such as the antiviral effects of Isatis root polysaccharides against porcine reproductive and respiratory syndrome virus [39] or the possible anticoagulant and antithrombotic effects of the venom of the snake Deinagkistrodon acutus [40]. Potential pharmacological effects were also found by Xie et al who associated the antipyretic mechanism to ellagic acid in rats [41,42].…”