Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

Sawaya, Alexandra Christine Helena Frankland; Souza, Kirllian S.; Marcucci, María Cristina; Cunha, Ildenize B. S.; Shimizu, M. T.

doi:10.1590/s1517-83822004000100017

Cited by 50 publications

(55 citation statements)

References 17 publications

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“…With respect to our results, since most of compounds in propolis are resin and wax, high concentrations of alcohol could be helpful for releasing soluble compounds from this sticky part. This experience was also reported previously (Sawaya et al 2004). After complete solubilization of propolis, addition of water or buffer to decrease ethanol down to 50% helps to keep the soluble compounds in solution.…”

Section: Discussionsupporting

confidence: 77%

Effect of the water extracts of propolis on stimulation and inhibition of different cells

et al. 2007

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The water extracts of propolis (WEP) could inhibit growth of different cell lines namely McCoy, HeLa, SP2/0, HEp-2, and BHK21 and stimulate growth of normal cell named human lymphocyte, rat kidney, rat liver, and rat spleen. In these experiments 1 and 2 mg of WEP were added to 1 ml RPMI media with 5% FCS. Cell counts and cell viability of propolis-treated and propolis-free cells were assessed by Trypan blue dye exclusion test and MTT assay. The results showed that in case of McCoy, HeLa, SP20, HEp-2, and BHK21 cell lines, the water extracts of propolis could inhibit cell growth as well as reduction on size of the cells. In contrast the same amount of WEP could stimulate growth of normal cells up to 60% with the same concentration used for cell lines. Thus our study indicates that although WEP consists only of the soluble part of propolis, it enables to inhibit different cell lines and increase growth of normal cells. This indicates also that WEP contains the specific compounds with bioactivity against cell lines. Although propolis contain different number of compounds it is clear that WEP has enough biological compounds useful for the treatment of some diseases, medical and related applications.

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Section: Discussionsupporting

confidence: 77%

Effect of the water extracts of propolis on stimulation and inhibition of different cells

et al. 2007

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“…SDA overlay TLC bioautography method (Sawaya ACHF., 2004) was used to detect the effective antifungal compounds from Caesalpinia coriaria. The inoculum of fungi was distributed over the already prepared TLC plate.…”

Section: Identification Of the Antifungal Compounds On Tlc Plate Thromentioning

confidence: 99%

Bioautography assay of Caesalpinia coriaria (Jacq) wild, as antifungal agent.

2016

Int. J. Curr. Res. Biol. Med.

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Bioautography identification of Caesalpinia coriaria as antifungal compounds on the TLC plates was studied. An important development of drug discovery process over the last several decades has been in the use of biological assay systems. Based on this the ethanolic extract of C.coriaria were subjected for bioautography assay. The fractions were tested for their antifungal activity against Mucor organism. Active compounds alkaloids, flavonoids, terpenoids and cadiac glycosides from the fractions are transferred from stationary phase to the agar layer (which contains the microorganisms) by a different process. After incubation the plate is sprayed with a tetrazolium salt (eg. MTT) which is converted to a formazan dye by the microorganism. Inhibition zones are observed as clear spots against a purple background with this knowledge, it is clear that in the present study the active compound fractions 12 at Rf showed significant inhibition towards the growth of pathogenic microorganisms. It showed the presence of bioactive compounds responsible as antifungal properties of ethanolic partially purified extract. However, this finding provides an insight into the usage of this plant in traditional treatment of foot infections, subcutaneous parasitic infection, intestinal parasitism, venereal diseases and other diseases associated with bacterial and fungal infections.

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“…Specific Gravity And Antibacterial Assays Of Synthetic Essential Oils Bioautography Assay: Prior to the minimum inhibitory concentration (MIC) determinations, bioautography assay was carried out for the SIEO samples according to method described by Sawaya et al, (2004) and Mendonça-Filho (2006). Thin layer chromatographic (TLC) plates, composed of Merck Silica gel 60 GF 254, received 5 µL of each SIEO sample placed at a distance from 1.5 cm of the lower edge of the plate.…”

Section: Full Length Research Articlementioning

confidence: 99%

Specific gravity and antibacterial assays of some synthetic industrial essential oils

Hati¹,

Dimari²,

Egwu³

et al. 2010

Science World Journal

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Samples of synthetic industrial essential oil (SIEO) were assayed to provide basis for establishing correlation and validation of claims of its density, disinfectant and germicidal properties with its natural counterparts. Specific gravity (SG) and antibacterial assays were carried out on twenty typical SIEO samples that are commonly used in Northern Nigeria. The results show that mean SG was 0.970 with 5% coefficient of variance (CV) between samples and was not statistically significant following Kolmogorov-Smirnov error protection at 95% confidence limit. Antimicrobial activity test results consisting of bioautography and minimum inhibitory concentration (MIC) tests present variable effects of the SIEO S. aureus and E. coli but are not completely dependent on each other. Generally, the results of SG determinations for SIEO correspond greatly with SG values of most natural essential oils extracts and that up 70% SIEO showed antibacterial activity. The SIEO studied like their natural counterpart may contain certain antibacterial agents with similar effects to standard Chloramphenicol used in this work. However, further studies are required to justify the safety of the application of SIEO as antimicrobial agents.

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Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria

Cited by 50 publications

References 17 publications

Effect of the water extracts of propolis on stimulation and inhibition of different cells

Effect of the water extracts of propolis on stimulation and inhibition of different cells

Bioautography assay of Caesalpinia coriaria (Jacq) wild, as antifungal agent.

Specific gravity and antibacterial assays of some synthetic industrial essential oils

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