Analysis of the Composition of Lyophilisates Obtained from Aloe arborescens Gel of Leaves of Different Ages from Controlled Crops

Abstract

The aim of the study is to evaluate the composition of lyophilisates obtained from Aloe arborescens leaf gel at the age of one to four years. The leaves were obtained from controlled crops, which allowed to exclude environmental factors as variables. It was confirmed that the lyophilisates obtained from different years of Aloe arborescens leaf gel varied in chromatographic analyses in terms of aloin A and aloenin A content (high-performance liquid chromatography with diode-array detection HPLC-DAD, high-performance liquid chromatography with tandem mass spectrometric detection HPLC-MS/MS). Similarly, while testing the phenolic acids and the sum of polyphenols content, differences in their levels in leaf gel lyophilisates from plants of individual years were observed (spectrophotometric method UV-VIS). The lyophilisate composition analysis showed that the one-year-old leaves were characterized by the highest content of aloin A and aloenin A. While the content of polyphenols, including phenolic acids, was higher in the leaves of older plants. The antioxidant potential of the tested lyophilisates was assessed simultaneously. Regardless of the research model used (CUPRAC, DPPH, ABTS), an antioxidant effect was noted for Aloe arborescens leaves.

Aloe arborescens: In Vitro Screening of Genotoxicity, Effective Inhibition of Enzyme Characteristics for Disease Etiology, and Microbiological Activity

“…This research is a continuation of our previous studies, which included the analysis of the content of aloin A, aloenin A, polyphenolic compounds, and phenolic acids in raw material, and the evaluation of the antioxidant activity of extracts from the freeze-dried leaf pulp of A. arborescens from 1-, 2-, 3- and 4-year-old plants. The effects of the study mentioned above can be found in one of our published papers [ 23 ]. This manuscript presents the results of our research on the lyophilizate from 3-year-old plants, which turned out to exhibit a higher activity and height content of active compounds ( Table 1 ).…”

“…Our previous research on the plant material Aloe arborescens showed that the lyophilisates obtained from the pulp of 3-year-old leaves contain the highest polyphenol content, which translates into the highest antioxidant activity against the DPPH radical [ 23 ]. Bearing in mind the results obtained earlier, we focused on assessing the safety and activity potential of lyophilisates from 3-year-old Aloe arborescens leaves in the current research.…”

“…Thus, A. arborescens , despite being less popular than Aloe vera , shows significant biological activity and thus has high research potential to accurately determine its activity profile and the possibility of using it as a medicinal raw material. Previous research has investigated how the age of A. arborescens affects the content of aloin A, aloenin A, polyphenolic compounds, and phenolic acids [ 23 ].…”

Towards the Preparation of a Hydrogel from Lyophilisates of the Aloe arborescens Aqueous Extract

“…The concentrations of main active compounds (aloin A and aloenin A) were determined by using the HPLC-Diode-Array Detection method (equipment LC system, Dionex Thermoline Fisher Scientific) with Chromeleon software version 7.0) described previously [ 20 ]. Briefly, separations were performed on a LiChrospher RP-18 column, 5 μm particle size, 250 mm × 4 mm LiChroCART ® 250-4 (Merck, Darmstadt, Germany).…”

“…In the first phase of the research, we compared the composition of leaves obtained from one, two, three and four-year-old plants. Leaves obtained from three-year-old plants were indicated as the most worthwhile as they had the highest total phenolic concentration and total antioxidant activity [ 20 ]. For them, ethanol extracts were obtained, for which the safety of their use and effectiveness in relation to fibroblasts was also proven [ 21 ].…”