Analysis of the costs for the laboratory of flow cytometry screening of urine samples before culture

Paattiniemi, Eeva-Liisa; Karumaa, Santra; Viita, Anna-Mari; Kärpänoja, Pauliina; Mākelä, Marjukka; Isojärvi, Jaana; Sarkkinen, Hannu

doi:10.1080/23744235.2016.1239028

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…Although studies have shown an economic benefit of the use of urine flow cytometry before urine culture [ 34 ], the impact on the prescription of antibiotics remains unknown.…”

Section: Discussionmentioning

confidence: 99%

The development and validation of different decision-making tools to predict urine culture growth out of urine flow cytometry parameter

et al. 2018

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ObjectivePatients presenting with suspected urinary tract infection are common in every day emergency practice. Urine flow cytometry has replaced microscopic urine evaluation in many emergency departments, but interpretation of the results remains challenging. The aim of this study was to develop and validate tools that predict urine culture growth out of urine flow cytometry parameter.MethodsThis retrospective study included all adult patients that presented in a large emergency department between January and July 2017 with a suspected urinary tract infection and had a urine flow cytometry as well as a urine culture obtained. The objective was to identify urine flow cytometry parameters that reliably predict urine culture growth and mixed flora growth. The data set was split into a training (70%) and a validation set (30%) and different decision-making approaches were developed and validated.ResultsRelevant urine culture growth (respectively mixed flora growth) was found in 40.2% (7.2% respectively) of the 613 patients included. The number of leukocytes and bacteria in flow cytometry were highly associated with urine culture growth, but mixed flora growth could not be sufficiently predicted from the urine flow cytometry parameters. A decision tree, predictive value figures, a nomogram, and a cut-off table to predict urine culture growth from bacteria and leukocyte count were developed, validated and compared.ConclusionsUrine flow cytometry parameters are insufficient to predict mixed flora growth. However, the prediction of urine culture growth based on bacteria and leukocyte count is highly accurate and the developed tools should be used as part of the decision-making process of ordering a urine culture or starting an antibiotic therapy if a urogenital infection is suspected.

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“…Although studies have shown an economic benefit of the use of urine flow cytometry before urine culture [ 34 ], the impact on the prescription of antibiotics remains unknown.…”

Section: Discussionmentioning

confidence: 99%

The development and validation of different decision-making tools to predict urine culture growth out of urine flow cytometry parameter

et al. 2018

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“…As suggested by different studies [20, 24, 25], one strategy for establishing cut-off values is to maximize Youden's index. Our results suggest that this strategy does not lead to useful cut-offs in many clinical presentations, as the resulting cut-offs can neither be used to “rule in” (high LR(+)) nor be used to “rule out” (LR(-)) significant bacterial growth in the urine culture but lead to balanced cut-offs with regard to LR(+)/LR(-).…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Urinary Tract Infections by Urine Flow Cytometry: Adjusted Cut-Off Values in Different Clinical Presentations

et al. 2019

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Background. Bacterium and leucocyte counts in urine can be measured by urine flow cytometry (UFC). They are used to predict significant bacterial growth in urine culture and to diagnose infections of the urinary tract. However, little information is available on appropriate UFC cut-off values for bacterium and leucocyte counts in specific clinical presentations. Objective. To develop, validate, and evaluate adapted cut-off values that result in a high negative predictive value for significant bacterial growth in urine culture in common clinical presentation subgroups. Methods. This is a single center, retrospective, observational study with data from patients of the emergency department of Bern University Hospital, Switzerland, with suspected infections of the urinary tract. The patients presented with different symptoms, and urine culture and urine flow cytometry were performed. For different clinical presentations, the patients were grouped by (i) age (>65 years), (ii) sex, (iii) clinical symptoms (e.g., fever or dysuria), and (iv) comorbidities such as diabetes and immunosuppression. For each group, cut-off values were developed, validated, and analyzed using different strategies, i.e., linear discriminant analysis (LDA) and Youden’s index, and were compared with known cut-offs and cut-offs optimized for sensitivity. Results. 613 patients were included in the study. Significant bacterial growth in urine culture depended on clinical presentation and ranged from 32.3% in male patients to 61.5% in patients with urinary frequency. In all clinical presentations, the predictive accuracy of UFC leucocyte and UFC bacterium counts was good for significant bacterial growth in urine culture (AUC≥0.88). The adapted LDA95 equations did not exhibit consistently high sensitivity. However, the in-house cut-offs (test positive if UFC leucocytes>17/μL or UFC bacteria>125/μL) were highly sensitive (>90%). In female, younger, and dysuric patients, even higher cut-offs for UFC leucocytes (169/μL, 169/μL, and 205/μL) exhibited high sensitivity. Specificity was insufficient (<0.9) for all tested cut-offs. Conclusions. For various clinical presentations, significant bacterial growth in urine culture can be excluded if flow cytometry measurements give a bacterial count of ≤125/μL or a leucocyte count of ≤17/μL. In female patients, dysuric patients, and patients younger than ≤65 years, the leucocyte cut-off can be increased to 170/μL.

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“…The reliability of this approach is an ongoing subject of discussion [ 3 ]. In recent years, urine flow cytometry (UFC) has been evaluated as a reliable and efficient method to diagnose bacteriuria, revealing heterogeneous results in different patient populations [ 4 , 5 , 6 ]. Patients scheduled for surgery are of particular interest, as asymptomatic bacteriuria is of relevance in urothelium-traumatizing urological procedures [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Urine Flow Cytometry on the UF-1000i System and Urine Culture of Urine Samples from Urological Patients

et al. 2021

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Introduction: The aims of this study were to evaluate urine flow cytometry (UFC) as a tool to screen urine samples of urological patients for bacteriuria and to compare UFC and dipstick analysis with urine culture in a patient cohort at a urological department of a university hospital. Methods and Material: We screened 662 urine samples from urological patients (75.2% male; 80.7% inpatients; mean age 58 years). UFC results were compared to microbiological urine culture. Results: The accuracy in using the UFC-based parameters for detecting cultural bacteriuria was 91.99% and 88.97% for ≥105 colony-forming units (CFU)/mL and ≥104 CFU/mL, respectively. UFC and leukocyte dipstick analysis measured leukocyturia similarly (Pearson correlation coefficient 0.87, p value <0.01%), but dipstick analysis scored less accurately on bacteriuria (accuracy 59.37% and 62.69%). UFC remained effective in subgroup analysis of patients of both sexes and with different urological conditions with its overall use only slightly impaired when assessing gross hematuria (NPV 84.62% for ≥104 CFU/mL). UFC also reliably removed those urine samples below cutoffs with negative predictive values of 99.28% for ≥105 CFU/mL and 95.86% for ≥104 CFU/mL. Conclusion: Counting bacteria with UFC is an accurate and rapid method to determine significant bacteriuria in urological patients and is superior to dipstick analysis or indirect surrogate parameters such as leukocyturia. When UFC is available, we recommend it to be used for the diagnosis of bacteriuria over findings obtained by dipstick analysis.

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Analysis of the costs for the laboratory of flow cytometry screening of urine samples before culture

Cited by 6 publications

References 22 publications

The development and validation of different decision-making tools to predict urine culture growth out of urine flow cytometry parameter

The development and validation of different decision-making tools to predict urine culture growth out of urine flow cytometry parameter

Diagnosis of Urinary Tract Infections by Urine Flow Cytometry: Adjusted Cut-Off Values in Different Clinical Presentations

Comparison of Urine Flow Cytometry on the UF-1000i System and Urine Culture of Urine Samples from Urological Patients

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