Purpose The study of phosphorus (P) transfer from soils to rivers is a subject of interest as there is a clear relation between land use and water quality. P transfer in the soil/sediment system was evaluated by comparing the concentrations of total (P T ) and bioavailable P (P A ). Materials and methods Phosphorus concentrations were measured in three different compartments following the natural transport route in a basin: soils, suspended and riverbed sediments. Soils were sampled from five different types of land cover: cropped land, forest, pasture, river banks and road taluses (roadside open earthen ditches). Samples were taken from two different sub-basins: the Grande and the upper Anllóns sub-basins. Results and discussion The highest concentrations of P were found in the suspended sediments, followed by the riverbed sediments and the soils. The cropped and pasture soils contained the highest amounts of P. The P A /P ratio was very low in the soils (<0.3), but was significantly higher in the sediments, particularly in the suspended sediments (0.67). The concentration of P A was also significantly higher in the suspended sediments and riverbed sediments than in the river basin soils, with concentration factors of 300% for P and 2,600% for P A , either in suspended and bed sediments being slightly higher in the suspended than in the bed sediments , which suggests the potential environmental risk of P transfer to the river courses via soil. Conclusions Comprehensive studies involving P transfer across soils-suspended sediments-bed sediments are scarce, highlighting the innovative nature of the present study. The results obtained will allow the evaluation of the impact of different types of land use on the sustainability of the aquatic ecosystem at a catchment scale.