Analysis of the Differential Gene and Protein Expression Profiles of Corneal Epithelial Cells Stimulated with Alternating Current Electric Fields

Kowtharapu, Bhavani S.; Damaraju, Jyoti; Singh, Nitesh Kumar; Ziebart, Josefin; Bader, Rainer; Koczan, Dirk; Stachs, Oliver

doi:10.3390/genes12020299

Cited by 9 publications

(5 citation statements)

References 61 publications

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“…As soon as the PNI occurs, endogenous electric fields are generated correspondingly, which may take part in regulating the rate of nerve sprouting, growth, and regeneration. Applied electric fields have likewise been found to influence the regeneration of nerves after injury, i.e., promote survival, migration, and axonal elongation of neurons, either applied immediately following nerve repair or a perioperative ES ( Zarrintaj et al, 2020 ; Kowtharapu et al, 2021 ; Trueman et al, 2022 ). In 1952, Hoffman was the first to apply ES to injured nerve.…”

Section: Application Of Es For Treatment Of Pnimentioning

confidence: 99%

Peripheral nerve injury repair by electrical stimulation combined with graphene-based scaffolds

Zhao,

Liu,

Kang

et al. 2024

Front. Bioeng. Biotechnol.

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Peripheral nerve injury (PNI) is a common clinical problem, which due to poor recovery often leads to limb dysfunction and sensory abnormalities in patients. Tissue-engineered nerve guidance conduits (NGCs) that are designed and fabricated from different materials are the potential alternative to nerve autografts. However, translation of these NGCs from lab to commercial scale has not been well achieved. Complete functional recovery with the aid of NGCs in PNI becomes a topic of general interest in tissue engineering and regeneration medicine. Electrical stimulation (ES) has been widely used for many years as an effective physical method to promote nerve repair in both pre-clinical and clinical settings. Similarly, ES of conductive and electroactive materials with a broad range of electrical properties has been shown to facilitate the guidance of axons and enhance the regeneration. Graphene and its derivatives possess unique physicochemical and biological properties, which make them a promising outlook for the development of synthetic scaffolds or NGCs for PNI repair, especially in combination with ES. Considering the discussion regarding ES for the treatment of PNI must continue into further detail, herein, we focus on the role of ES in PNI repair and the molecular mechanism behind the ES therapy for PNI, providing a summary of recent advances in context of graphene-based scaffolds (GBSs) in combination with ES. Future perspectives and some challenges faced in developing GBSs are also highlighted with the aim of promoting their clinical applications.

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Section: Application Of Es For Treatment Of Pnimentioning

confidence: 99%

Peripheral nerve injury repair by electrical stimulation combined with graphene-based scaffolds

Zhao,

Liu,

Kang

et al. 2024

Front. Bioeng. Biotechnol.

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“…32 It is a Shiny application built with many R/Bioconductor programs and a large annotation and pathway database extracted from a variety of sources. 28, 29 It is intended for in-depth gene list analysis, with graphical enrichment visualization and pathway analysis (p-value < 0.05). The hierarchical clustering tree summarizes the relationship between the significant pathways listed in the enrichment tab.…”

Section: Go and Pathway Enrichment Analysismentioning

confidence: 99%

“…6a, b). 29 In clinical application, we found that arsenic resistance genes (arsB, arsC, and arsR) in multidrug-resistant KP and PA can be valuable for researchers in several ways to develop therapeutic use. Salam et al 57 in 2020 studied arsenic resistance genes provided insights into cross-resistance mechanisms between arsenic and antibiotics.…”

Section: Go and Pathway Enrichment Analysismentioning

confidence: 99%

Identification and Exploration of Transcripts Involved in Antibiotic Resistance Mechanism of Two Critical Superbugs

Sahoo,

Mohanty,

Routray

et al. 2024

Gene Expr

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Background and objectives: Infectious diseases caused by pathogenic strains of bacteria are a global cause of morbidity and mortality. Hospital-acquired infections caused by Klebsiella pneumonia and Pseudomonas aeruginosa were found vulnerable during the COVID-19 pandemic. They are also responsible for the onset of certain life-threatening infectious diseases such as cystic fibrosis, endocarditis, bacteremia, and sepsis. Looking into the importance of these two superbugs there is a strong need for extensive comparative differential gene expression analysis among the wild-type and mutant for betterment of intensive care unit patients especially as such pathogenic bacterial strains have a dangerous role in the intensive care unit.Methods: This study revealed the RNA microarray gene expression profiles of GSE24688, GSE4026, and GSE117438. The study compared all genes from three different datasets and all drug resistance genes from two divergent organisms, Klebsiella pneumonia and Pseudomonas aeruginosa.Results: 10 numbers of shared significant genes and five drug resistance genes were obtained in this study. These putative genes may show intriguing patterns of connection with resistance mechanisms and can be used in the field of diagnostics and treatment. Our divergent analysis also revealed a very clear distinct relation between Klebsiella pneumoniae and Pseudomonas aeruginosa at the genetic level, though they both function under antimicrobial resistance. Conclusions:This study enhances the understanding of the genetic basis, providing valuable knowledge for the development of new strategies to combat antibiotic resistance and enhance the efficacy of existing antibiotics.

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“…The disease alliance analysis showed arteriosclerosis, renal cell carcinoma, Alzheimer's, hypertension, breast cancer, urinary bladder cancer, Parkinson's disease, myocardial infarction, breast carcinoma, etc. The KEGG analysis showed Nitrogen metabolism one of the processes of plant physiology and also one of the important parts of global chemical cycle which can lead to lung adenocarcinoma, prolactin signaling pathway is activated by prolactin-PRLR interaction is the JAK/STAT pathway that can cause tumorigenesis, reproductive abnormalities, and diabetes, Cell cycle is a series of events that takes place in a cell as it grows and divides which may lead to Glaucoma and other retinal disorders, lL-17 signaling pathway initiates signaling through Act1-induced K63-linked ubiquitylation of TRAF6 which can cause diseases such as psoriasis, rheumatoid arthritis (RA), multiple sclerosis, scleroderma, and apoptosis are known to be a form of planned cell death that occurs in multicellular organisms which can lead to Alzheimer's disease, Huntington's disease, and Parkinson's disease [27].…”

Section: Discusssionmentioning

confidence: 99%

Network Pharmacology and Molecular Docking-Based Prediction of Pharmacological Properties of Osthole

DIVEKAR

2023

Innovare J Med Sci

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Objectives: In this study, the term called network pharmacology (NP) process which was used to understand the underlying mechanism of the pharmacological properties of Osthole. NP is developed that is used to understand drug actions and interactions with multiple targets and it is also capable of completely articulating the complexity between diseases and medications. The research was carried out for the identification of diverse drug-target interactions using NP to discover novel medications for difficult conditions such as Parkinson’s, Cancer, and Alzheimer’s disease and many more. Osthole was used for prediction which could be used in the pharmaceutical background. Methods: To understand the binding affinity of Osthole with the corresponding target proteins, it was analyzed. It was determined from the pathway by which diseases can be caused, such as cancer and Alzheimer’s disease. A PyRx tool was used to carry out the molecular docking. For this research, structures of protein and phytocompounds were retrieved from UniProtKB and PubChem. Furthermore, along with the help of BIOVIA discovery studio software, the protein structure was analyzed and ADMET screening was done to evaluate the Osthole pharmacological properties. Results: The ligands were retrieved for Osthole from PubChem, then target prediction was carried out where it showed 100 potential targets. The protein-protein network and interaction were done using the STRING database, in which it showed that these CREBBP, IDO1, and MAPK8 targets have maximum interactions followed by the Gene functional analysis, that is, go function and KEGG pathway. The molecular docking was carried out using PyRx in which 4U72 showed the best binding affinity to Osthole. Furthermore, visualization was done using BIOVIA Discovery Studio, which provided the 3D and 2D visualization. Conclusion: According to the results obtained for molecular docking, these target proteins have pharmacological effects which can be considered as suggestions for the investigation of the pharmacological mechanism of Osthole.

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Analysis of the Differential Gene and Protein Expression Profiles of Corneal Epithelial Cells Stimulated with Alternating Current Electric Fields

Cited by 9 publications

References 61 publications

Peripheral nerve injury repair by electrical stimulation combined with graphene-based scaffolds

Peripheral nerve injury repair by electrical stimulation combined with graphene-based scaffolds

Identification and Exploration of Transcripts Involved in Antibiotic Resistance Mechanism of Two Critical Superbugs

Network Pharmacology and Molecular Docking-Based Prediction of Pharmacological Properties of Osthole

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