Analysis of the distribution of bacteria within urinary catheter biofilms using four different molecular techniques

Choe, Hyun‐Sop; Son, Su-Wan; Choi, Hyun-Woo; Kim, Hyun-Jung; Ahn, Sun-Gook; Bang, Jun-Ho; Lee, Seung‐Ju; Lee, Jiyoul; Cho, Yong-Hyun; Lee, Sang-Seob

doi:10.1016/j.ajic.2012.05.010

Cited by 30 publications

(35 citation statements)

References 35 publications

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“…Biofilm-related infections are considered a major cause of morbidity and mortality in hospitals (36). Corynebacterium is known as a biofilm producing pathogen and is responsible for reduced susceptibility to antibiotics (37,38). To the best of our knowledge, this is the first report of variable biofilm formation by different C. striatum strains isolated from blood specimens.…”

Section: Discussionmentioning

confidence: 87%

Characteristics of Multidrug-Resistant <i>Corynebacterium</i> spp. Isolated from Blood Cultures of Hospitalized Patients in Japan

et al. 2017

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Section: Discussionmentioning

confidence: 87%

Characteristics of Multidrug-Resistant <i>Corynebacterium</i> spp. Isolated from Blood Cultures of Hospitalized Patients in Japan

et al. 2017

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“…Some studies showed that Achromobacter spp. can form biofilm on surfaces other than CL, such as central venous catheters and urinary catheters [15,16]. The presence of biofilms in sputum from cystic fibrosis (CF) patients infected with A. xylosoxidans was revealed in one study.…”

Section: Discussionmentioning

confidence: 99%

Biofilm formation of Achromobacter xylosoxidans on contact lens

Konstantinović

Ćirković

Đukić

et al. 2017

Acta Microbiologica et Immunologica Hungarica

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Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated strain of A. xylosoxidans and reference strains of P. aeruginosa, S. aureus, and H. influenzae on soft CL were analyzed by commonly used microtitre plate method. Our results showed that isolated strain of A. xylosoxidans was capable to form biofilm on the surface of soft contact lens. A. xylosoxidans was strong biofilm producer while all examined reference strains were moderate biofilm producers. A. xylosoxidans appears to be superior biofilm producer on soft CL compared to reference strains.

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“…Sample-specific amplicon patterns are analysed when the fingerprint approach is applied. However, subsequently separated amplicon sequencing is usually required for taxonomical characterization in clinical samples [Choe et al, 2012;Siqueira et al, 2004]. Despite some known limitations, particularly multitemplate PCR and PCR-DGGE bias, this technique has already been used for the identification of bacteria in consortia of urinary catheters [Choe et al, 2012].…”

Section: Contribution Of Pcr Denaturing Gradientmentioning

confidence: 99%

“…There are possible strategies to overcome this issue: presequencing amplicon separation using enhanced electrophoretic techniques, such as denaturing gradient gel electrophoresis (DGGE) [Choe et al, 2012;Li et al, 2005;Siqueira et al, 2004], postsequencing software separation of up to 3 particular electropherograms by the RipSeq Mixed web-based application (Pathogenomix Inc., USA) [Kommedal et al, 2009], or next-generation sequencing (NGS). NGS is a revolutionary technology, particularly useful for highly diverse bacterial consortia (e.g., stool samples) and/or a high-throughput setting.…”

Section: Contribution Of Pcr Denaturing Gradientmentioning

confidence: 99%

Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis

et al. 2017

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Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR- DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment.

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Analysis of the distribution of bacteria within urinary catheter biofilms using four different molecular techniques

Cited by 30 publications

References 35 publications

Characteristics of Multidrug-Resistant <i>Corynebacterium</i> spp. Isolated from Blood Cultures of Hospitalized Patients in Japan

Characteristics of Multidrug-Resistant <i>Corynebacterium</i> spp. Isolated from Blood Cultures of Hospitalized Patients in Japan

Biofilm formation of Achromobacter xylosoxidans on contact lens

Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis

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