Analysis of the effect of different withering methods on tea quality based on transcriptomics and metabolomics

Jia, Xiaoli; Zhang, Qi; Chen, Meihui; Wang, Yuhua; Lin, Shaoxiong; Pan, Yibin; Cheng, Pengyuan; Li, Mingzhe; Zhang, Ying; Ye, Jianghua; Wang, Haibin

doi:10.3389/fpls.2023.1235687

Cited by 12 publications

(7 citation statements)

References 42 publications

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“…The content of flavonoids and soluble sugars was determined by aluminum trichloride colorimetry and anthrone colorimetry, respectively . At the same time, tea taste was converted according to the content of different quality indexes obtained from the determination with reference to the taste characteristics of each substance (Table S1) (Jia et al, 2023).…”

Section: Determination Of Tea Quality Indexesmentioning

confidence: 99%

Transcriptomic analysis of the effect of shaking and tumbling degree on quality formation of Wuyi rock tea

Chen,

Zhang,

Wang

et al. 2023

Journal of Food Science

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Shaking and tumbling are extremely important for the formation of the special flavor of Wuyi rock tea. In this study, we analyzed the effects of different shaking and tumbling degrees on the quality index content of tea leaves and determined changes in gene expression in tea leaves using RNA sequencing technology. On this basis, the correlation between gene expression intensities in tea leaves and tea quality index content was analyzed. The results showed that heavy shaking and tumbling (MW3) increased gene expression of metabolic pathways, biosynthesis of secondary metabolites, starch and sucrose metabolism, biosynthesis of amino acids, glycine, serine, and threonine metabolism, alpha‐linolenic acid metabolism pathways and decreased gene expression of flavonoid biosynthesis, carbon fixation in photosynthetic organisms, phenylpropanoid biosynthesis, and plant hormone signal transduction pathways in tea leaves, which in turn increased the content of caffeine, soluble sugar, amino acid and decreased the content of flavone, tea polyphenol, catechin component in tea leaves; the opposite was true for light shaking and tumbling. Second, this study found that MW3 was more beneficial in improving the mellowness, sweetness, and fresh and brisk taste of tea leaves and reducing the bitterness of tea leaves. This study provides some references to guide the processing of Wuyi rock tea with different flavors.Practical ApplicationHeavy shaking and tumbling was more beneficial in improving the mellowness, sweetness, and fresh and brisk taste of tea leaves and reducing the bitterness of tea leaves. Therefore, the degree of shaking and tumbling in Wuyi production can be appropriately improved to produce high‐quality tea and improve the economic benefits of tea.

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Section: Determination Of Tea Quality Indexesmentioning

confidence: 99%

Transcriptomic analysis of the effect of shaking and tumbling degree on quality formation of Wuyi rock tea

Chen,

Zhang,

Wang

et al. 2023

Journal of Food Science

Self Cite

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“…IBM SPSS Statistics 21.0 software was used for data T-text and correlation analysis. R version 4.2.3 software was used to produce box plots (R libraries was gghalves version 0.1.4), principal component analysis (PCA, R libraries was ggbiplot version 0.55), heat plots (R libraries was pheatmap 1.0.12), volcano plot analysis (R libraries was ggplot2 version 3.4.0), orthogonal partial least squares discriminant analysis (OPLS-DA, package used for this was ropls and mixOmics) ( Jia et al., 2023 ), redundancy analysis (RDA, R libraries was vegan version 2.6.4) and correlation matrix analysis (R libraries was linkET 0.0.7.1) ( Watkins, 2020 ). The entropy weight TOPSIS statistical analysis was performed on the SPSSAU online platform ( https://spssau.com/ ) ( Çelikbilek and Tüysüz, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of continuous planting on Casuarina equisetifolia rhizosphere soil physicochemical indexes, microbial functional diversity and metabolites

Wang,

Li,

et al. 2023

Front. Plant Sci.

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Continuous planting has a severe impact on the growth of Casuarina equisetifolia. In this study, the effects of three different long-term monocultures (one, two and three replanting) on the physicochemical indexes, microbial functional diversity, and soil metabolomics were analyzed in C. equisetifolia rhizosphere soil. The results showed that rhizosphere soil organic matter content, cation exchange capacity, total and available nitrogen, total and available phosphorus, and total and available potassium contents significantly decreased with the increasing number of continuous plantings. The evaluation of microbial functional diversity revealed a reduction in the number of soil microorganisms that rely on carbohydrates for carbon sources and an increase in soil microorganisms that used phenolic acid, carboxylic acid, fatty acid, and amines as carbon sources. Soil metabolomics analysis showed a significant decrease in soil carbohydrate content and a significant accumulation of autotoxic acid, amine, and lipid in the C. equisetifolia rhizosphere soil. Consequently, the growth of C. equisetifolia could hinder total nutrient content and their availability. Thus, valuable insights for managing the cultivation of C. equisetifolia and soil remediation were provided.

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“…Based on this, the OPLS-DA model was further constructed in this study ( Zhang et al., 2023c ). After the goodness-of-fit and predictability of the model were assessed to be qualified, key microorganisms were screened by the important projected value (VIP value) of the model indexes ( Jia et al., 2023b ; Zhang et al., 2023b ). In addition, XGBoost machine deep learning was applied to model construction to obtain the importance of key microorganisms in distinguishing different samples ( Chen et al., 2020 ; Khan et al., 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation

Wang,

Lin,

et al. 2023

Front. Plant Sci.

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Casuarina equisetifolia (C. equisetifolia) is an economically important forest tree species, often cultivated in continuous monoculture as a coastal protection forest. Continuous planting has gradually affected growth and severely restricted the sustainable development of the C. equisetifolia industry. In this study, we analyzed the effects of continuous planting on C. equisetifolia growth and explored the rhizosphere soil microecological mechanism from a metagenomic perspective. The results showed that continuous planting resulted in dwarfing, shorter root length, and reduced C. equisetifolia seedling root system. Metagenomics analysis showed that 10 key characteristic microorganisms, mainly Actinoallomurus, Actinomadura, and Mycobacterium, were responsible for continuously planted C. equisetifolia trees. Quantitative analysis showed that the number of microorganisms in these three genera decreased significantly with the increase of continuous planting. Gene function analysis showed that continuous planting led to the weakening of the environmental information processing-signal transduction ability of soil characteristic microorganisms, and the decrease of C. equisetifolia trees against stress. Reduced capacity for metabolism, genetic information processing-replication and repair resulted in reduced microbial propagation and reduced microbial quantity in the rhizosphere soil of C. equisetifolia trees. Secondly, amino acid metabolism, carbohydrate metabolism, glycan biosynthesis and metabolism, lipid metabolism, metabolism of cofactors and vitamins were all significantly reduced, resulting in a decrease in the ability of the soil to synthesize and metabolize carbon and nitrogen. These reduced capacities further led to reduced soil microbial quantity, microbial carbon and nitrogen, microbial respiration intensity, reduced soil enzyme nutrient cycling and resistance-related enzyme activities, a significant reduction in available nutrient content of rhizosphere soils, a reduction in the ion exchange capacity, and an impediment to C. equisetifolia growth. This study provides an important basis for the management of continuously planted C. equisetifolia plantations.

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Analysis of the effect of different withering methods on tea quality based on transcriptomics and metabolomics

Cited by 12 publications

References 42 publications

Transcriptomic analysis of the effect of shaking and tumbling degree on quality formation of Wuyi rock tea

Transcriptomic analysis of the effect of shaking and tumbling degree on quality formation of Wuyi rock tea

Effect of continuous planting on Casuarina equisetifolia rhizosphere soil physicochemical indexes, microbial functional diversity and metabolites

Metagenomics-based exploration of key soil microorganisms contributing to continuously planted Casuarina equisetifolia growth inhibition and their interactions with soil nutrient transformation

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